Influence of temperature, pH and naloxone on the antinociceptive activity of Channa striatus (haruan) extracts in mice
We have previously shown that extracts from the snakehead fish, Channa striatus (haruan), have antinociceptive activity on their own, and enhance morphine antinociception. In furtherance of that study, we investigated the effects of temperature, pH and naloxone on the antinociceptive effects of haru...
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Elsevier
1999
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| Online Access: | http://psasir.upm.edu.my/id/eprint/112583/ http://psasir.upm.edu.my/id/eprint/112583/3/112583.pdf |
| _version_ | 1848865981297852416 |
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| author | Dambisya, Yoswa M. Lee, Tat-Leang Sathivulu, Vijayakumar Mat Jais, Abdul Manan |
| author_facet | Dambisya, Yoswa M. Lee, Tat-Leang Sathivulu, Vijayakumar Mat Jais, Abdul Manan |
| author_sort | Dambisya, Yoswa M. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | We have previously shown that extracts from the snakehead fish, Channa striatus (haruan), have antinociceptive activity on their own, and enhance morphine antinociception. In furtherance of that study, we investigated the effects of temperature, pH and naloxone on the antinociceptive effects of haruan extracts in the mouse abdominal constriction test. The effects of temperature were only studied on mucus extracts since the fillet extract is prepared at high temperature under pressure. There was a general loss of inhibitory activity from 88.4% at 0 °C to 61.1% at 80-100 °C, though this was only significant at 80 °C. The effects of pH were studied on both extracts. Lowering the mucus extract pH to 6.0 (unadjusted pH 7.4) did not significantly affect its activity, while raising it to 8.0 attenuated its activity. Similarly, pH changes had no effect on the fillet extract except at pH 8.0 which produced apparently lower antinociception. Pretreatment with naloxone (4 mg/kg) had no significant effect on the inhibitory effects of either extract in the abdominal constriction test. These results suggest that the active ingredient(s) of these extracts is (are) relatively stable over a wide range of temperature and between pH 6.0 and 8.0. That would be consistent with the possibility of a glycoprotein, polypeptide or polysaccharide. |
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| format | Article |
| id | upm-112583 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:13:20Z |
| publishDate | 1999 |
| publisher | Elsevier |
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| spelling | upm-1125832025-03-06T04:04:00Z http://psasir.upm.edu.my/id/eprint/112583/ Influence of temperature, pH and naloxone on the antinociceptive activity of Channa striatus (haruan) extracts in mice Dambisya, Yoswa M. Lee, Tat-Leang Sathivulu, Vijayakumar Mat Jais, Abdul Manan We have previously shown that extracts from the snakehead fish, Channa striatus (haruan), have antinociceptive activity on their own, and enhance morphine antinociception. In furtherance of that study, we investigated the effects of temperature, pH and naloxone on the antinociceptive effects of haruan extracts in the mouse abdominal constriction test. The effects of temperature were only studied on mucus extracts since the fillet extract is prepared at high temperature under pressure. There was a general loss of inhibitory activity from 88.4% at 0 °C to 61.1% at 80-100 °C, though this was only significant at 80 °C. The effects of pH were studied on both extracts. Lowering the mucus extract pH to 6.0 (unadjusted pH 7.4) did not significantly affect its activity, while raising it to 8.0 attenuated its activity. Similarly, pH changes had no effect on the fillet extract except at pH 8.0 which produced apparently lower antinociception. Pretreatment with naloxone (4 mg/kg) had no significant effect on the inhibitory effects of either extract in the abdominal constriction test. These results suggest that the active ingredient(s) of these extracts is (are) relatively stable over a wide range of temperature and between pH 6.0 and 8.0. That would be consistent with the possibility of a glycoprotein, polypeptide or polysaccharide. Elsevier 1999 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/112583/3/112583.pdf Dambisya, Yoswa M. and Lee, Tat-Leang and Sathivulu, Vijayakumar and Mat Jais, Abdul Manan (1999) Influence of temperature, pH and naloxone on the antinociceptive activity of Channa striatus (haruan) extracts in mice. Journal of Ethnopharmacology, 66 (2). pp. 181-186. ISSN 0378-8741; eISSN: 1872-7573 https://linkinghub.elsevier.com/retrieve/pii/S037887419800169X 10.1016/s0378-8741(98)00169-x |
| spellingShingle | Dambisya, Yoswa M. Lee, Tat-Leang Sathivulu, Vijayakumar Mat Jais, Abdul Manan Influence of temperature, pH and naloxone on the antinociceptive activity of Channa striatus (haruan) extracts in mice |
| title | Influence of temperature, pH and naloxone on the antinociceptive activity of Channa striatus (haruan) extracts in mice |
| title_full | Influence of temperature, pH and naloxone on the antinociceptive activity of Channa striatus (haruan) extracts in mice |
| title_fullStr | Influence of temperature, pH and naloxone on the antinociceptive activity of Channa striatus (haruan) extracts in mice |
| title_full_unstemmed | Influence of temperature, pH and naloxone on the antinociceptive activity of Channa striatus (haruan) extracts in mice |
| title_short | Influence of temperature, pH and naloxone on the antinociceptive activity of Channa striatus (haruan) extracts in mice |
| title_sort | influence of temperature, ph and naloxone on the antinociceptive activity of channa striatus (haruan) extracts in mice |
| url | http://psasir.upm.edu.my/id/eprint/112583/ http://psasir.upm.edu.my/id/eprint/112583/ http://psasir.upm.edu.my/id/eprint/112583/ http://psasir.upm.edu.my/id/eprint/112583/3/112583.pdf |