Physical parameters affecting transient GUS gene expression in oil palm (Elaeis guineensis Jacq.) using the biolistic device
Physical parameters for DNA delivery into oil palm embryogenic calli using the biolistic device have been successfully established. Helium pressures, distance from rupture disc to macrocarrier, distance from macrocarrier to stopping plate, distance from stopping plate to target tissue, vacuum pressu...
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| Format: | Article |
| Language: | English |
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Elsevier
1997
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| Online Access: | http://psasir.upm.edu.my/id/eprint/112537/ http://psasir.upm.edu.my/id/eprint/112537/3/112537.pdf |
| _version_ | 1848865969816993792 |
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| author | Parveez, Ghulam Kadir A. Chowdhury, M.K.U. M. Saleh, Norihan |
| author_facet | Parveez, Ghulam Kadir A. Chowdhury, M.K.U. M. Saleh, Norihan |
| author_sort | Parveez, Ghulam Kadir A. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Physical parameters for DNA delivery into oil palm embryogenic calli using the biolistic device have been successfully established. Helium pressures, distance from rupture disc to macrocarrier, distance from macrocarrier to stopping plate, distance from stopping plate to target tissue, vacuum pressures, number of bombardments, particle types and sizes and the effect of calcium chloride and spermidine on microcarrier-DNA binding have been optimizied. Embryogenic calli were bombarded with gold or tungsten particles coated with pEmuGN plasmid containing β-glucuronidase gene (uidA) driven by an Emu promoter. A completely randomized design was used to bombard a total of 200 plates (40 parameters x 5 replications) which were then incubated in a growth chamber at 28°C. Two days after bombardment, the tissues were stained with GUS assay buffer for 16-20 h at 37°C and the blue spots were counted under a binocular microscope. Analysis of variance (ANOVA) and Duncan's new multiple range test were used to analyze data from each experiment. All the variables used in this experiment were significantly different except for vacuum pressures and bombardment numbers. |
| first_indexed | 2025-11-15T14:13:09Z |
| format | Article |
| id | upm-112537 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:13:09Z |
| publishDate | 1997 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1125372025-03-06T06:32:31Z http://psasir.upm.edu.my/id/eprint/112537/ Physical parameters affecting transient GUS gene expression in oil palm (Elaeis guineensis Jacq.) using the biolistic device Parveez, Ghulam Kadir A. Chowdhury, M.K.U. M. Saleh, Norihan Physical parameters for DNA delivery into oil palm embryogenic calli using the biolistic device have been successfully established. Helium pressures, distance from rupture disc to macrocarrier, distance from macrocarrier to stopping plate, distance from stopping plate to target tissue, vacuum pressures, number of bombardments, particle types and sizes and the effect of calcium chloride and spermidine on microcarrier-DNA binding have been optimizied. Embryogenic calli were bombarded with gold or tungsten particles coated with pEmuGN plasmid containing β-glucuronidase gene (uidA) driven by an Emu promoter. A completely randomized design was used to bombard a total of 200 plates (40 parameters x 5 replications) which were then incubated in a growth chamber at 28°C. Two days after bombardment, the tissues were stained with GUS assay buffer for 16-20 h at 37°C and the blue spots were counted under a binocular microscope. Analysis of variance (ANOVA) and Duncan's new multiple range test were used to analyze data from each experiment. All the variables used in this experiment were significantly different except for vacuum pressures and bombardment numbers. Elsevier 1997 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/112537/3/112537.pdf Parveez, Ghulam Kadir A. and Chowdhury, M.K.U. and M. Saleh, Norihan (1997) Physical parameters affecting transient GUS gene expression in oil palm (Elaeis guineensis Jacq.) using the biolistic device. Industrial Crops and Products, 6 (1). pp. 41-50. ISSN 0926-6690; eISSN: 1872-633X https://linkinghub.elsevier.com/retrieve/pii/S092666909600204X 10.1016/s0926-6690(96)00204-x |
| spellingShingle | Parveez, Ghulam Kadir A. Chowdhury, M.K.U. M. Saleh, Norihan Physical parameters affecting transient GUS gene expression in oil palm (Elaeis guineensis Jacq.) using the biolistic device |
| title | Physical parameters affecting transient GUS gene expression in oil palm (Elaeis guineensis Jacq.) using the biolistic device |
| title_full | Physical parameters affecting transient GUS gene expression in oil palm (Elaeis guineensis Jacq.) using the biolistic device |
| title_fullStr | Physical parameters affecting transient GUS gene expression in oil palm (Elaeis guineensis Jacq.) using the biolistic device |
| title_full_unstemmed | Physical parameters affecting transient GUS gene expression in oil palm (Elaeis guineensis Jacq.) using the biolistic device |
| title_short | Physical parameters affecting transient GUS gene expression in oil palm (Elaeis guineensis Jacq.) using the biolistic device |
| title_sort | physical parameters affecting transient gus gene expression in oil palm (elaeis guineensis jacq.) using the biolistic device |
| url | http://psasir.upm.edu.my/id/eprint/112537/ http://psasir.upm.edu.my/id/eprint/112537/ http://psasir.upm.edu.my/id/eprint/112537/ http://psasir.upm.edu.my/id/eprint/112537/3/112537.pdf |