Effect of promoter strength and signal sequence on the periplasmic expression of human interferon-α2b in Escherichia coli

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Effect of promoter strength and signal sequence on the periplasmic expression of human interferon-α2b in Escherichia coli

Two plasmids, pFLAG-ATS and pET 26b(+), were studied for the periplasmic expression of recombinant human interferon-α2b (IFN-α2b) in Escherichia coli. The pFLAG-ATS contains ompA signal sequence and tac promoter while pET 26b(+) contains pelB signal sequence and T7lac promoter. It was observed that...

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Bibliographic Details
Main Authors:	Ramanan, Ramakrishnan Nagasundara, Wong, Bee Tik, Memari, Hamid Rajabi, Azaman, Siti Nor Ani, Tau, Chuan Ling, Tey, Beng Ti, Mohd Lila, Mohd Azmi, Abdullah, Mohd Puad, Abdul Rahim, Raha, Ariff, Arbakariya
Format:	Article
Language:	English
Published:	Academic Journals 2010
Online Access:	http://psasir.upm.edu.my/id/eprint/11248/ http://psasir.upm.edu.my/id/eprint/11248/1/62.pdf

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