A proposal for zero emission from palm oil industry incorporating the production of polyhydroxyalkanoates from palm oil mill effluent

In our previous reports, we have described the production of organic acids from palm oil mill effluent (POME) which were used as fermentation substrates to produce polyhydroxyalkanoates (PHA). Here we propose a zero emission from palm oil industry incorporating the production of PHA from POME. Our r...

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Main Authors: Hassan, Mohd. Ali, Nawata, Osamu, Shirai, Yoshihito, Abdul Rahman, Nor Aini, Yee, Phang Lai, Ariff, Arbakariya, Abdul Karim, Mohamed Ismail
Format: Article
Published: Taylor and Francis Group 2002
Online Access:http://psasir.upm.edu.my/id/eprint/112388/
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author Hassan, Mohd. Ali
Nawata, Osamu
Shirai, Yoshihito
Abdul Rahman, Nor Aini
Yee, Phang Lai
Ariff, Arbakariya
Abdul Karim, Mohamed Ismail
author_facet Hassan, Mohd. Ali
Nawata, Osamu
Shirai, Yoshihito
Abdul Rahman, Nor Aini
Yee, Phang Lai
Ariff, Arbakariya
Abdul Karim, Mohamed Ismail
author_sort Hassan, Mohd. Ali
building UPM Institutional Repository
collection Online Access
description In our previous reports, we have described the production of organic acids from palm oil mill effluent (POME) which were used as fermentation substrates to produce polyhydroxyalkanoates (PHA). Here we propose a zero emission from palm oil industry incorporating the production of PHA from POME. Our results showed that by evaporation, the organic acids could be concentrated to about 100 g·I -1 for use as substrates for the fed-batch PHA fermentation. Upon condensation of the steam, the water had a COD of 80 ppm, low enough for it to be either recycled or discharged. It was confirmed that the energy for the evaporation can be provided by combusting solid wastes such as shells and empty oil palm bunches. The concentrated organic acids were successfully converted to PHA by Ralstonia eutropha strain ATCC 17699 under a non-sterile fermentation system when the initial cell density was kept high at 4 g·I -1. After 150 hours, 20 g·I -1 cells were obtained with more than 50% PHA content. A repeated fed-batch system was also performed to obtain a high cell inoculum and to mimic the operation of a large PHA production fermentor at C/N ratios of 15 and 30 respectively, with only acetic and propionic acids as carbon sources. It was suggested that the energy for this proposed process could be sufficiently supplied by combustion of the solid wastes from the palm oil mill.
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spelling upm-1123882025-02-19T08:28:24Z http://psasir.upm.edu.my/id/eprint/112388/ A proposal for zero emission from palm oil industry incorporating the production of polyhydroxyalkanoates from palm oil mill effluent Hassan, Mohd. Ali Nawata, Osamu Shirai, Yoshihito Abdul Rahman, Nor Aini Yee, Phang Lai Ariff, Arbakariya Abdul Karim, Mohamed Ismail In our previous reports, we have described the production of organic acids from palm oil mill effluent (POME) which were used as fermentation substrates to produce polyhydroxyalkanoates (PHA). Here we propose a zero emission from palm oil industry incorporating the production of PHA from POME. Our results showed that by evaporation, the organic acids could be concentrated to about 100 g·I -1 for use as substrates for the fed-batch PHA fermentation. Upon condensation of the steam, the water had a COD of 80 ppm, low enough for it to be either recycled or discharged. It was confirmed that the energy for the evaporation can be provided by combusting solid wastes such as shells and empty oil palm bunches. The concentrated organic acids were successfully converted to PHA by Ralstonia eutropha strain ATCC 17699 under a non-sterile fermentation system when the initial cell density was kept high at 4 g·I -1. After 150 hours, 20 g·I -1 cells were obtained with more than 50% PHA content. A repeated fed-batch system was also performed to obtain a high cell inoculum and to mimic the operation of a large PHA production fermentor at C/N ratios of 15 and 30 respectively, with only acetic and propionic acids as carbon sources. It was suggested that the energy for this proposed process could be sufficiently supplied by combustion of the solid wastes from the palm oil mill. Taylor and Francis Group 2002 Article PeerReviewed Hassan, Mohd. Ali and Nawata, Osamu and Shirai, Yoshihito and Abdul Rahman, Nor Aini and Yee, Phang Lai and Ariff, Arbakariya and Abdul Karim, Mohamed Ismail (2002) A proposal for zero emission from palm oil industry incorporating the production of polyhydroxyalkanoates from palm oil mill effluent. Journal of Chemical Engineering of Japan, 35 (1). pp. 9-14. ISSN 0021-9592; eISSN: 1881-1299 https://www.jstage.jst.go.jp/article/jcej/35/1/35_1_9/_article 10.1252/jcej.35.9
spellingShingle Hassan, Mohd. Ali
Nawata, Osamu
Shirai, Yoshihito
Abdul Rahman, Nor Aini
Yee, Phang Lai
Ariff, Arbakariya
Abdul Karim, Mohamed Ismail
A proposal for zero emission from palm oil industry incorporating the production of polyhydroxyalkanoates from palm oil mill effluent
title A proposal for zero emission from palm oil industry incorporating the production of polyhydroxyalkanoates from palm oil mill effluent
title_full A proposal for zero emission from palm oil industry incorporating the production of polyhydroxyalkanoates from palm oil mill effluent
title_fullStr A proposal for zero emission from palm oil industry incorporating the production of polyhydroxyalkanoates from palm oil mill effluent
title_full_unstemmed A proposal for zero emission from palm oil industry incorporating the production of polyhydroxyalkanoates from palm oil mill effluent
title_short A proposal for zero emission from palm oil industry incorporating the production of polyhydroxyalkanoates from palm oil mill effluent
title_sort proposal for zero emission from palm oil industry incorporating the production of polyhydroxyalkanoates from palm oil mill effluent
url http://psasir.upm.edu.my/id/eprint/112388/
http://psasir.upm.edu.my/id/eprint/112388/
http://psasir.upm.edu.my/id/eprint/112388/