Evaluation of different promoters driving the GFP reporter gene and selected target tissues for particle bombardment of Dendrobium Sonia 17
Three different morphological callus types, identified as type A, B and C, and tips of in vitro inflorescences were used as target tissues for genetic transformation. Five different DNA plasmids carrying a synthetic green fluorescent protein (gfp) gene driven by different promoters, CaMV 35S, HBT, a...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Springer
2003
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| Online Access: | http://psasir.upm.edu.my/id/eprint/112386/ http://psasir.upm.edu.my/id/eprint/112386/3/112386.pdf |
| _version_ | 1848865928928821248 |
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| author | Tee, C. M., Marziah Tan, C. Abdullah, M. |
| author_facet | Tee, C. M., Marziah Tan, C. Abdullah, M. |
| author_sort | Tee, C. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Three different morphological callus types, identified as type A, B and C, and tips of in vitro inflorescences were used as target tissues for genetic transformation. Five different DNA plasmids carrying a synthetic green fluorescent protein (gfp) gene driven by different promoters, CaMV 35S, HBT, and Ubi1 were tested for the genetic transformation of Dendrobium Sonia 17. 35S-sgfp-TYG-nos (p35S) with the CaMV 35S promoter showed the highest GFP transient expression rate, while the HBT and Ubi1 promoters showed a relatively lower expression rate in all of the target tissues tested. The highest number of GFP-expressing cells was observed on day 2 post-bombardment, and the number declined gradually over the course of the next 2 weeks. Type A and B callus were found to be the best potential target tissues for genetic transformation. |
| first_indexed | 2025-11-15T14:12:30Z |
| format | Article |
| id | upm-112386 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:12:30Z |
| publishDate | 2003 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1123862025-03-07T02:19:42Z http://psasir.upm.edu.my/id/eprint/112386/ Evaluation of different promoters driving the GFP reporter gene and selected target tissues for particle bombardment of Dendrobium Sonia 17 Tee, C. M., Marziah Tan, C. Abdullah, M. Three different morphological callus types, identified as type A, B and C, and tips of in vitro inflorescences were used as target tissues for genetic transformation. Five different DNA plasmids carrying a synthetic green fluorescent protein (gfp) gene driven by different promoters, CaMV 35S, HBT, and Ubi1 were tested for the genetic transformation of Dendrobium Sonia 17. 35S-sgfp-TYG-nos (p35S) with the CaMV 35S promoter showed the highest GFP transient expression rate, while the HBT and Ubi1 promoters showed a relatively lower expression rate in all of the target tissues tested. The highest number of GFP-expressing cells was observed on day 2 post-bombardment, and the number declined gradually over the course of the next 2 weeks. Type A and B callus were found to be the best potential target tissues for genetic transformation. Springer 2003 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/112386/3/112386.pdf Tee, C. and M., Marziah and Tan, C. and Abdullah, M. (2003) Evaluation of different promoters driving the GFP reporter gene and selected target tissues for particle bombardment of Dendrobium Sonia 17. Plant Cell Reports, 21 (5). pp. 452-458. ISSN 0721-7714; eISSN: 1432-203X https://link.springer.com/article/10.1007/s00299-002-0539-8?error=cookies_not_supported&code=737d329f-277b-4d50-a8bf-b042d46a7556 10.1007/s00299-002-0539-8 |
| spellingShingle | Tee, C. M., Marziah Tan, C. Abdullah, M. Evaluation of different promoters driving the GFP reporter gene and selected target tissues for particle bombardment of Dendrobium Sonia 17 |
| title | Evaluation of different promoters driving the GFP reporter gene and selected target tissues for particle bombardment of Dendrobium Sonia 17 |
| title_full | Evaluation of different promoters driving the GFP reporter gene and selected target tissues for particle bombardment of Dendrobium Sonia 17 |
| title_fullStr | Evaluation of different promoters driving the GFP reporter gene and selected target tissues for particle bombardment of Dendrobium Sonia 17 |
| title_full_unstemmed | Evaluation of different promoters driving the GFP reporter gene and selected target tissues for particle bombardment of Dendrobium Sonia 17 |
| title_short | Evaluation of different promoters driving the GFP reporter gene and selected target tissues for particle bombardment of Dendrobium Sonia 17 |
| title_sort | evaluation of different promoters driving the gfp reporter gene and selected target tissues for particle bombardment of dendrobium sonia 17 |
| url | http://psasir.upm.edu.my/id/eprint/112386/ http://psasir.upm.edu.my/id/eprint/112386/ http://psasir.upm.edu.my/id/eprint/112386/ http://psasir.upm.edu.my/id/eprint/112386/3/112386.pdf |