Oral and topical administration of a geranyl acetophenone attenuates DNCB‑induced atopic dermatitis‑like skin lesions in BALB/c mice
Atopic dermatitis (AD) is a chronic, allergic inflammatory skin disorder that lacks a definite cure. Using a mouse DNCB-induced AD-like skin lesions model, this study evaluated the potential therapeutic utility of tHGA as an oral and topical treatment for AD. Male BALB/c mice were sensitised and cha...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Research
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/112239/ http://psasir.upm.edu.my/id/eprint/112239/1/tHGA%20on%20DNCB%20Induced%20Atopic%20Deramatitis.pdf |
| _version_ | 1848865890614902784 |
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| author | Mohd Kasim, Vivi Nur Khalieda Lee, Yu Zhao Bakrin, Ikmal Hisyam Hussain, Mohd Khairi Ahmad Israf, Daud Shaari, Khozirah Tan, Ji Wei Lee, Ming Tatt Tham, Chau Ling |
| author_facet | Mohd Kasim, Vivi Nur Khalieda Lee, Yu Zhao Bakrin, Ikmal Hisyam Hussain, Mohd Khairi Ahmad Israf, Daud Shaari, Khozirah Tan, Ji Wei Lee, Ming Tatt Tham, Chau Ling |
| author_sort | Mohd Kasim, Vivi Nur Khalieda |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Atopic dermatitis (AD) is a chronic, allergic inflammatory skin disorder that lacks a definite cure. Using a mouse DNCB-induced AD-like skin lesions model, this study evaluated the potential therapeutic utility of tHGA as an oral and topical treatment for AD. Male BALB/c mice were sensitised and challenged with 1% and 0.5% DNCB on their shaved dorsal skin. Mice in the treatment group were administered tHGA (20, 40, and 80 mg/kg) orally three times per week for 2 weeks, or tHGA (0.2%, 1%, and 5%) topically once daily for 12 days. On day 34, the mice were euthanized, and blood and dorsal skin samples were obtained for analysis. All doses of orally and topically administered tHGA significantly improved scratching, epidermal thickness, blood eosinophilia and mast cell infiltration. There was a minor discrepancy between the two routes of administration, with orally treated tHGA showing significant reductions in Scoring of Atopic Dermatitis (SCORAD), tissue eosinophil infiltration, serum IgE and skin IL-4 levels with treatment of 40 and 80 mg/kg tHGA, whereas topically applied tHGA showed significant reductions in all dosages. These findings suggest that tHGA exhibited therapeutic potential for AD as both oral and topical treatment ameliorates AD-like symptoms in the murine model. © The Author(s) 2024. |
| first_indexed | 2025-11-15T14:11:54Z |
| format | Article |
| id | upm-112239 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:11:54Z |
| publishDate | 2024 |
| publisher | Nature Research |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1122392024-10-13T10:34:12Z http://psasir.upm.edu.my/id/eprint/112239/ Oral and topical administration of a geranyl acetophenone attenuates DNCB‑induced atopic dermatitis‑like skin lesions in BALB/c mice Mohd Kasim, Vivi Nur Khalieda Lee, Yu Zhao Bakrin, Ikmal Hisyam Hussain, Mohd Khairi Ahmad Israf, Daud Shaari, Khozirah Tan, Ji Wei Lee, Ming Tatt Tham, Chau Ling Atopic dermatitis (AD) is a chronic, allergic inflammatory skin disorder that lacks a definite cure. Using a mouse DNCB-induced AD-like skin lesions model, this study evaluated the potential therapeutic utility of tHGA as an oral and topical treatment for AD. Male BALB/c mice were sensitised and challenged with 1% and 0.5% DNCB on their shaved dorsal skin. Mice in the treatment group were administered tHGA (20, 40, and 80 mg/kg) orally three times per week for 2 weeks, or tHGA (0.2%, 1%, and 5%) topically once daily for 12 days. On day 34, the mice were euthanized, and blood and dorsal skin samples were obtained for analysis. All doses of orally and topically administered tHGA significantly improved scratching, epidermal thickness, blood eosinophilia and mast cell infiltration. There was a minor discrepancy between the two routes of administration, with orally treated tHGA showing significant reductions in Scoring of Atopic Dermatitis (SCORAD), tissue eosinophil infiltration, serum IgE and skin IL-4 levels with treatment of 40 and 80 mg/kg tHGA, whereas topically applied tHGA showed significant reductions in all dosages. These findings suggest that tHGA exhibited therapeutic potential for AD as both oral and topical treatment ameliorates AD-like symptoms in the murine model. © The Author(s) 2024. Nature Research 2024-07-31 Article PeerReviewed text en cc_by_nc_nd_4 http://psasir.upm.edu.my/id/eprint/112239/1/tHGA%20on%20DNCB%20Induced%20Atopic%20Deramatitis.pdf Mohd Kasim, Vivi Nur Khalieda and Lee, Yu Zhao and Bakrin, Ikmal Hisyam and Hussain, Mohd Khairi and Ahmad Israf, Daud and Shaari, Khozirah and Tan, Ji Wei and Lee, Ming Tatt and Tham, Chau Ling (2024) Oral and topical administration of a geranyl acetophenone attenuates DNCB‑induced atopic dermatitis‑like skin lesions in BALB/c mice. Scientific Reports, 14 (1). art. no. 17623. pp. 1-13. ISSN 2045-2322 https://www.nature.com/articles/s41598-024-66601-0 10.1038/s41598-024-66601-0 |
| spellingShingle | Mohd Kasim, Vivi Nur Khalieda Lee, Yu Zhao Bakrin, Ikmal Hisyam Hussain, Mohd Khairi Ahmad Israf, Daud Shaari, Khozirah Tan, Ji Wei Lee, Ming Tatt Tham, Chau Ling Oral and topical administration of a geranyl acetophenone attenuates DNCB‑induced atopic dermatitis‑like skin lesions in BALB/c mice |
| title | Oral and topical administration of a geranyl acetophenone attenuates DNCB‑induced atopic dermatitis‑like skin lesions in BALB/c mice |
| title_full | Oral and topical administration of a geranyl acetophenone attenuates DNCB‑induced atopic dermatitis‑like skin lesions in BALB/c mice |
| title_fullStr | Oral and topical administration of a geranyl acetophenone attenuates DNCB‑induced atopic dermatitis‑like skin lesions in BALB/c mice |
| title_full_unstemmed | Oral and topical administration of a geranyl acetophenone attenuates DNCB‑induced atopic dermatitis‑like skin lesions in BALB/c mice |
| title_short | Oral and topical administration of a geranyl acetophenone attenuates DNCB‑induced atopic dermatitis‑like skin lesions in BALB/c mice |
| title_sort | oral and topical administration of a geranyl acetophenone attenuates dncb‑induced atopic dermatitis‑like skin lesions in balb/c mice |
| url | http://psasir.upm.edu.my/id/eprint/112239/ http://psasir.upm.edu.my/id/eprint/112239/ http://psasir.upm.edu.my/id/eprint/112239/ http://psasir.upm.edu.my/id/eprint/112239/1/tHGA%20on%20DNCB%20Induced%20Atopic%20Deramatitis.pdf |