Heterophil to lymphocyte ratio and tonic immobility reactions to preslaughter handling in broiler chickens treated with ascorbic acid
Stress and fear responses were evaluated in broiler chicks that were pretreated for 24 h with 0 ppm (control) or 1,200 ppm of L-ascorbic acid (AA) in their drinking water. The birds were subsequently subjected to either upright handling (UH) or inverted (IH) handling for about 45 s. Heterophil (H) c...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2000
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/111951/ http://psasir.upm.edu.my/id/eprint/111951/3/111951.pdf |
| _version_ | 1848865820683272192 |
|---|---|
| author | Zulkifli, I. M.T., Che Norma Chong, C.H. Loh, T.C. |
| author_facet | Zulkifli, I. M.T., Che Norma Chong, C.H. Loh, T.C. |
| author_sort | Zulkifli, I. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Stress and fear responses were evaluated in broiler chicks that were pretreated for 24 h with 0 ppm (control) or 1,200 ppm of L-ascorbic acid (AA) in their drinking water. The birds were subsequently subjected to either upright handling (UH) or inverted (IH) handling for about 45 s. Heterophil (H) counts, lymphocyte (L) counts, and H/L ratios (H/L) ratios were determined immediately (T0) and at 20 h (T20) following the handling treatment. The H/L ratios were similar for both groups at T0, whereas 20 h after the handling treatment, AA-supplemented birds had lower ratios than controls, resulting in a significant water treatment × time of blood sampling interaction. Inverted handling had negligible effect on H/L ratios but augmented tonic immobility (TI) durations as compared with UH. Irrespective of handling procedure, supplemental AA reduced underlying fearfulness, as measured by TI reaction. Neither water treatment nor handling method had significant effect on number of attempts to induce TI. |
| first_indexed | 2025-11-15T14:10:47Z |
| format | Article |
| id | upm-111951 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:10:47Z |
| publishDate | 2000 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1119512025-03-11T06:37:13Z http://psasir.upm.edu.my/id/eprint/111951/ Heterophil to lymphocyte ratio and tonic immobility reactions to preslaughter handling in broiler chickens treated with ascorbic acid Zulkifli, I. M.T., Che Norma Chong, C.H. Loh, T.C. Stress and fear responses were evaluated in broiler chicks that were pretreated for 24 h with 0 ppm (control) or 1,200 ppm of L-ascorbic acid (AA) in their drinking water. The birds were subsequently subjected to either upright handling (UH) or inverted (IH) handling for about 45 s. Heterophil (H) counts, lymphocyte (L) counts, and H/L ratios (H/L) ratios were determined immediately (T0) and at 20 h (T20) following the handling treatment. The H/L ratios were similar for both groups at T0, whereas 20 h after the handling treatment, AA-supplemented birds had lower ratios than controls, resulting in a significant water treatment × time of blood sampling interaction. Inverted handling had negligible effect on H/L ratios but augmented tonic immobility (TI) durations as compared with UH. Irrespective of handling procedure, supplemental AA reduced underlying fearfulness, as measured by TI reaction. Neither water treatment nor handling method had significant effect on number of attempts to induce TI. Elsevier 2000 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/111951/3/111951.pdf Zulkifli, I. and M.T., Che Norma and Chong, C.H. and Loh, T.C. (2000) Heterophil to lymphocyte ratio and tonic immobility reactions to preslaughter handling in broiler chickens treated with ascorbic acid. Poultry Science, 79 (3). pp. 402-406. ISSN 0032-5791; eISSN: 1525-3171 https://linkinghub.elsevier.com/retrieve/pii/S0032579119414892 10.1093/ps/79.3.402 |
| spellingShingle | Zulkifli, I. M.T., Che Norma Chong, C.H. Loh, T.C. Heterophil to lymphocyte ratio and tonic immobility reactions to preslaughter handling in broiler chickens treated with ascorbic acid |
| title | Heterophil to lymphocyte ratio and tonic immobility reactions to preslaughter handling in broiler chickens treated with ascorbic acid |
| title_full | Heterophil to lymphocyte ratio and tonic immobility reactions to preslaughter handling in broiler chickens treated with ascorbic acid |
| title_fullStr | Heterophil to lymphocyte ratio and tonic immobility reactions to preslaughter handling in broiler chickens treated with ascorbic acid |
| title_full_unstemmed | Heterophil to lymphocyte ratio and tonic immobility reactions to preslaughter handling in broiler chickens treated with ascorbic acid |
| title_short | Heterophil to lymphocyte ratio and tonic immobility reactions to preslaughter handling in broiler chickens treated with ascorbic acid |
| title_sort | heterophil to lymphocyte ratio and tonic immobility reactions to preslaughter handling in broiler chickens treated with ascorbic acid |
| url | http://psasir.upm.edu.my/id/eprint/111951/ http://psasir.upm.edu.my/id/eprint/111951/ http://psasir.upm.edu.my/id/eprint/111951/ http://psasir.upm.edu.my/id/eprint/111951/3/111951.pdf |