Specificity and sensitivity of a rapid dipstick test (Brugia Rapid) in the detection of Brugia malayi infection
A total of 753 serum samples from 6 institutions in 3 countries (Malaysia, Indonesia and India) were used to evaluate an immunochromatographic rapid dipstick test, Brugia Rapid, for diagnosis of Brugia malayi infection. The samples comprised sera from 207 microfilaria-positive individuals and 546 in...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Oxford University Press
2001
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/111913/ http://psasir.upm.edu.my/id/eprint/111913/3/111913.pdf |
| _version_ | 1848865811380305920 |
|---|---|
| author | N., Rahmah S., Taniawati Shenoy, R.K. Lim, B.H. Kumaraswami, V. Anuar, A.Khairul Hakim, S.Lokman M.I., Noor Hayati Chan, B.T. M., Suharni Ramachandran, C.P. |
| author_facet | N., Rahmah S., Taniawati Shenoy, R.K. Lim, B.H. Kumaraswami, V. Anuar, A.Khairul Hakim, S.Lokman M.I., Noor Hayati Chan, B.T. M., Suharni Ramachandran, C.P. |
| author_sort | N., Rahmah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A total of 753 serum samples from 6 institutions in 3 countries (Malaysia, Indonesia and India) were used to evaluate an immunochromatographic rapid dipstick test, Brugia Rapid, for diagnosis of Brugia malayi infection. The samples comprised sera from 207 microfilaria-positive individuals and 546 individuals from filaria non-endemic areas. The latter consisted of 70 individuals with soil-transmitted helminth infections, 68 with other helminth infections, 238 with protozoan infections, 12 with bacterial and viral infections and 158 healthy individuals. The dipstick is prepared with a goat anti-mouse antibody control line and a B. malayi recombinant-antigen test line. First, the dipstick is dipped into a well containing diluted patient serum, thus allowing specific anti-filarial antibody in the serum to react with the recombinant antigen. Then the dipstick is placed into an adjacent well containing reconstituted anti-human IgG4-gold. After 10 min, development of 2 red-purplish lines denotes a positive result and one line indicates a negative reaction. The overall results of the evaluation showed 97% sensitivity, 99% specificity, 97% positive predictive value and 99% negative predictive value. Brugia Rapid is thus a promising diagnostic tool for detection of B. malayi infection, and would be especially useful for the brugian filariasis elimination programme. |
| first_indexed | 2025-11-15T14:10:38Z |
| format | Article |
| id | upm-111913 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:10:38Z |
| publishDate | 2001 |
| publisher | Oxford University Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1119132025-03-12T01:09:26Z http://psasir.upm.edu.my/id/eprint/111913/ Specificity and sensitivity of a rapid dipstick test (Brugia Rapid) in the detection of Brugia malayi infection N., Rahmah S., Taniawati Shenoy, R.K. Lim, B.H. Kumaraswami, V. Anuar, A.Khairul Hakim, S.Lokman M.I., Noor Hayati Chan, B.T. M., Suharni Ramachandran, C.P. A total of 753 serum samples from 6 institutions in 3 countries (Malaysia, Indonesia and India) were used to evaluate an immunochromatographic rapid dipstick test, Brugia Rapid, for diagnosis of Brugia malayi infection. The samples comprised sera from 207 microfilaria-positive individuals and 546 individuals from filaria non-endemic areas. The latter consisted of 70 individuals with soil-transmitted helminth infections, 68 with other helminth infections, 238 with protozoan infections, 12 with bacterial and viral infections and 158 healthy individuals. The dipstick is prepared with a goat anti-mouse antibody control line and a B. malayi recombinant-antigen test line. First, the dipstick is dipped into a well containing diluted patient serum, thus allowing specific anti-filarial antibody in the serum to react with the recombinant antigen. Then the dipstick is placed into an adjacent well containing reconstituted anti-human IgG4-gold. After 10 min, development of 2 red-purplish lines denotes a positive result and one line indicates a negative reaction. The overall results of the evaluation showed 97% sensitivity, 99% specificity, 97% positive predictive value and 99% negative predictive value. Brugia Rapid is thus a promising diagnostic tool for detection of B. malayi infection, and would be especially useful for the brugian filariasis elimination programme. Oxford University Press 2001 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/111913/3/111913.pdf N., Rahmah and S., Taniawati and Shenoy, R.K. and Lim, B.H. and Kumaraswami, V. and Anuar, A.Khairul and Hakim, S.Lokman and M.I., Noor Hayati and Chan, B.T. and M., Suharni and Ramachandran, C.P. (2001) Specificity and sensitivity of a rapid dipstick test (Brugia Rapid) in the detection of Brugia malayi infection. Transactions of the Royal Society of Tropical Medicine and Hygiene, 95 (6). pp. 601-604. ISSN 0035-9203; eISSN: 1878-3503 https://academic.oup.com/trstmh/article-abstract/95/6/601/1905924?redirectedFrom=fulltext 10.1016/s0035-9203(01)90091-4 |
| spellingShingle | N., Rahmah S., Taniawati Shenoy, R.K. Lim, B.H. Kumaraswami, V. Anuar, A.Khairul Hakim, S.Lokman M.I., Noor Hayati Chan, B.T. M., Suharni Ramachandran, C.P. Specificity and sensitivity of a rapid dipstick test (Brugia Rapid) in the detection of Brugia malayi infection |
| title | Specificity and sensitivity of a rapid dipstick test (Brugia Rapid) in the detection of Brugia malayi infection |
| title_full | Specificity and sensitivity of a rapid dipstick test (Brugia Rapid) in the detection of Brugia malayi infection |
| title_fullStr | Specificity and sensitivity of a rapid dipstick test (Brugia Rapid) in the detection of Brugia malayi infection |
| title_full_unstemmed | Specificity and sensitivity of a rapid dipstick test (Brugia Rapid) in the detection of Brugia malayi infection |
| title_short | Specificity and sensitivity of a rapid dipstick test (Brugia Rapid) in the detection of Brugia malayi infection |
| title_sort | specificity and sensitivity of a rapid dipstick test (brugia rapid) in the detection of brugia malayi infection |
| url | http://psasir.upm.edu.my/id/eprint/111913/ http://psasir.upm.edu.my/id/eprint/111913/ http://psasir.upm.edu.my/id/eprint/111913/ http://psasir.upm.edu.my/id/eprint/111913/3/111913.pdf |