Modulation of protease activity to enhance the recovery of recombinant nucleocapsid protein of Nipah virus
The nucleocapsid (N) protein of Nipah virus (NiV) expressed in Escherichia coli (E. coli) is antigenic and immunogenic. A method to enhance the recovery of recombinant N protein of NiV produced in E. coli is described. A bioinformatics tool, PeptideCutter was used to identify potential protease and...
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| Format: | Article |
| Language: | English English |
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Elsevier
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/11191/ http://psasir.upm.edu.my/id/eprint/11191/1/Modulation%20of%20protease%20activity%20to%20enhance%20the%20recovery%20of%20recombinant%20nucleocapsid%20protein%20of%20Nipah%20virus.pdf |
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| author | Chong, Fui Chin Tan, Wen Siang Awang Biak, Dayang Radiah Ling, Tau Chuan Tey, Beng Ti |
| author_facet | Chong, Fui Chin Tan, Wen Siang Awang Biak, Dayang Radiah Ling, Tau Chuan Tey, Beng Ti |
| author_sort | Chong, Fui Chin |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The nucleocapsid (N) protein of Nipah virus (NiV) expressed in Escherichia coli (E. coli) is antigenic and immunogenic. A method to enhance the recovery of recombinant N protein of NiV produced in E. coli is described. A bioinformatics tool, PeptideCutter was used to identify potential protease and cleavage sites from the amino acid sequences deduced from the published DNA sequence of the N protein of NiV. The size of degraded protein was estimated by using the Western blot and PeptideCutter analyse. The identified proteases were serine proteases, hence, a range of serine protease inhibitors were tested to improve the recovery of the N protein. The relative amount of N protein of NiV was 2-fold higher with the addition of PMSF, compared to the control sample (without any protease inhibitor supplementation).
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| first_indexed | 2025-11-15T07:45:30Z |
| format | Article |
| id | upm-11191 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T07:45:30Z |
| publishDate | 2010 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-111912015-11-02T06:29:28Z http://psasir.upm.edu.my/id/eprint/11191/ Modulation of protease activity to enhance the recovery of recombinant nucleocapsid protein of Nipah virus Chong, Fui Chin Tan, Wen Siang Awang Biak, Dayang Radiah Ling, Tau Chuan Tey, Beng Ti The nucleocapsid (N) protein of Nipah virus (NiV) expressed in Escherichia coli (E. coli) is antigenic and immunogenic. A method to enhance the recovery of recombinant N protein of NiV produced in E. coli is described. A bioinformatics tool, PeptideCutter was used to identify potential protease and cleavage sites from the amino acid sequences deduced from the published DNA sequence of the N protein of NiV. The size of degraded protein was estimated by using the Western blot and PeptideCutter analyse. The identified proteases were serine proteases, hence, a range of serine protease inhibitors were tested to improve the recovery of the N protein. The relative amount of N protein of NiV was 2-fold higher with the addition of PMSF, compared to the control sample (without any protease inhibitor supplementation). Elsevier 2010-01 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/11191/1/Modulation%20of%20protease%20activity%20to%20enhance%20the%20recovery%20of%20recombinant%20nucleocapsid%20protein%20of%20Nipah%20virus.pdf Chong, Fui Chin and Tan, Wen Siang and Awang Biak, Dayang Radiah and Ling, Tau Chuan and Tey, Beng Ti (2010) Modulation of protease activity to enhance the recovery of recombinant nucleocapsid protein of Nipah virus. Process Biochemistry, 45 (1). pp. 133-137. ISSN 1359-5113 10.1016/j.procbio.2009.08.012 English |
| spellingShingle | Chong, Fui Chin Tan, Wen Siang Awang Biak, Dayang Radiah Ling, Tau Chuan Tey, Beng Ti Modulation of protease activity to enhance the recovery of recombinant nucleocapsid protein of Nipah virus |
| title | Modulation of protease activity to enhance the recovery of recombinant nucleocapsid protein of Nipah virus |
| title_full | Modulation of protease activity to enhance the recovery of recombinant nucleocapsid protein of Nipah virus |
| title_fullStr | Modulation of protease activity to enhance the recovery of recombinant nucleocapsid protein of Nipah virus |
| title_full_unstemmed | Modulation of protease activity to enhance the recovery of recombinant nucleocapsid protein of Nipah virus |
| title_short | Modulation of protease activity to enhance the recovery of recombinant nucleocapsid protein of Nipah virus |
| title_sort | modulation of protease activity to enhance the recovery of recombinant nucleocapsid protein of nipah virus |
| url | http://psasir.upm.edu.my/id/eprint/11191/ http://psasir.upm.edu.my/id/eprint/11191/ http://psasir.upm.edu.my/id/eprint/11191/1/Modulation%20of%20protease%20activity%20to%20enhance%20the%20recovery%20of%20recombinant%20nucleocapsid%20protein%20of%20Nipah%20virus.pdf |