Low level phosphorus supply affecting nodulation, N2 fixation and growth of cowpea (Vigna unguiculata L. Walp)
The interactive effect of low P supply (0, 10, 20 and 40 μM) and plant age on nodule number, mass and functioning (ureide analysis technique), vegetative growth and pod production were investigated in glasshouse-grown nodulated cowpea (Vigna unguiculata L.cv. Kausband) in sand culture. Compared with...
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| Format: | Article |
| Language: | English |
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Springer
1991
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| Online Access: | http://psasir.upm.edu.my/id/eprint/111897/ http://psasir.upm.edu.my/id/eprint/111897/3/111897.pdf |
| _version_ | 1848865808624648192 |
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| author | Wan Othman, W. M. Lie, T. A. Mannetje, L. 't Wassink, G. Y. |
| author_facet | Wan Othman, W. M. Lie, T. A. Mannetje, L. 't Wassink, G. Y. |
| author_sort | Wan Othman, W. M. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The interactive effect of low P supply (0, 10, 20 and 40 μM) and plant age on nodule number, mass and functioning (ureide analysis technique), vegetative growth and pod production were investigated in glasshouse-grown nodulated cowpea (Vigna unguiculata L.cv. Kausband) in sand culture. Compared with 40 μM P, P stress (0 μM P) or very low (10 μM P) supply markedly impaired nodulation, allantoin and amino-N concentrations and weight of N solutes in xylem exudates. Consequently, P stress reduced top growth and pod yields by 48 and 90%, respectively. N solutes in xylem exudates and total plant N assayed by Kjeldahl technique (as estimates of N2 fixation) responded similarly to P supply. However, the relative ureide index [(ureide-N/ureide N+amino-N)×100] remained constant (99%), irrespective of P supply, indicating the plants' complete dependency on symbiosis for growth, without implying that growth was markedly increased by N2 fixation. Although P concentrations in plant tops, roots and nodules increased with P supply, N concentrations in these plant tissues were unaffected by P supply. The concentrations of N and P in the nodules were 2–2 1/2 times higher than in plant tops. P application interacted strongly with plant age, with the largest P effect evidently achieved at the early podding stage. The significance and implications of these results are discussed. |
| first_indexed | 2025-11-15T14:10:36Z |
| format | Article |
| id | upm-111897 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:10:36Z |
| publishDate | 1991 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1118972025-03-12T01:14:49Z http://psasir.upm.edu.my/id/eprint/111897/ Low level phosphorus supply affecting nodulation, N2 fixation and growth of cowpea (Vigna unguiculata L. Walp) Wan Othman, W. M. Lie, T. A. Mannetje, L. 't Wassink, G. Y. The interactive effect of low P supply (0, 10, 20 and 40 μM) and plant age on nodule number, mass and functioning (ureide analysis technique), vegetative growth and pod production were investigated in glasshouse-grown nodulated cowpea (Vigna unguiculata L.cv. Kausband) in sand culture. Compared with 40 μM P, P stress (0 μM P) or very low (10 μM P) supply markedly impaired nodulation, allantoin and amino-N concentrations and weight of N solutes in xylem exudates. Consequently, P stress reduced top growth and pod yields by 48 and 90%, respectively. N solutes in xylem exudates and total plant N assayed by Kjeldahl technique (as estimates of N2 fixation) responded similarly to P supply. However, the relative ureide index [(ureide-N/ureide N+amino-N)×100] remained constant (99%), irrespective of P supply, indicating the plants' complete dependency on symbiosis for growth, without implying that growth was markedly increased by N2 fixation. Although P concentrations in plant tops, roots and nodules increased with P supply, N concentrations in these plant tissues were unaffected by P supply. The concentrations of N and P in the nodules were 2–2 1/2 times higher than in plant tops. P application interacted strongly with plant age, with the largest P effect evidently achieved at the early podding stage. The significance and implications of these results are discussed. Springer 1991 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/111897/3/111897.pdf Wan Othman, W. M. and Lie, T. A. and Mannetje, L. 't and Wassink, G. Y. (1991) Low level phosphorus supply affecting nodulation, N2 fixation and growth of cowpea (Vigna unguiculata L. Walp). Plant and Soil, 135 (1). pp. 67-74. ISSN 0032-079X; eISSN: 1573-5036 https://link.springer.com/article/10.1007/BF00014779?error=cookies_not_supported&code=9807747e-714e-4d24-b914-0a3e30b5b05a 10.1007/bf00014779 |
| spellingShingle | Wan Othman, W. M. Lie, T. A. Mannetje, L. 't Wassink, G. Y. Low level phosphorus supply affecting nodulation, N2 fixation and growth of cowpea (Vigna unguiculata L. Walp) |
| title | Low level phosphorus supply affecting nodulation, N2 fixation and growth of cowpea (Vigna unguiculata L. Walp) |
| title_full | Low level phosphorus supply affecting nodulation, N2 fixation and growth of cowpea (Vigna unguiculata L. Walp) |
| title_fullStr | Low level phosphorus supply affecting nodulation, N2 fixation and growth of cowpea (Vigna unguiculata L. Walp) |
| title_full_unstemmed | Low level phosphorus supply affecting nodulation, N2 fixation and growth of cowpea (Vigna unguiculata L. Walp) |
| title_short | Low level phosphorus supply affecting nodulation, N2 fixation and growth of cowpea (Vigna unguiculata L. Walp) |
| title_sort | low level phosphorus supply affecting nodulation, n2 fixation and growth of cowpea (vigna unguiculata l. walp) |
| url | http://psasir.upm.edu.my/id/eprint/111897/ http://psasir.upm.edu.my/id/eprint/111897/ http://psasir.upm.edu.my/id/eprint/111897/ http://psasir.upm.edu.my/id/eprint/111897/3/111897.pdf |