Novel peptides that inhibit the propagation of Newcastle disease virus
A disulfide constrained random heptapeptide library displayed on filamentous bacteriophage M13 was applied to select specific ligands that interact with Newcastle disease virus (NDV). A fusion phage carrying the amino acid sequence TLTTKLY was selected from the panning procedure. An antibody competi...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Published: |
Springer
2002
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/111867/ |
| _version_ | 1848865799993819136 |
|---|---|
| author | Ramanujam, P. Tan, W. S. Nathan, S. Yusoff, K. |
| author_facet | Ramanujam, P. Tan, W. S. Nathan, S. Yusoff, K. |
| author_sort | Ramanujam, P. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A disulfide constrained random heptapeptide library displayed on filamentous bacteriophage M13 was applied to select specific ligands that interact with Newcastle disease virus (NDV). A fusion phage carrying the amino acid sequence TLTTKLY was selected from the panning procedure. An antibody competition assay showed that the selected phage was capable of competing with the polyclonal antibodies raised against NDV for binding sites on the virus. Determination of the binding affinity of this phage with NDV by an equilibrium binding assay in solution revealed two different dissociation constants, suggesting that there could be two distinct binding sites for the phage on NDV. Synthetic peptides with the sequence CTLTTKLYC, either in linear or cyclic conformations inhibited the binding of phage bearing the same sequence to NDV. These peptides also inhibited the hemolytic activity of the virus as well as its propagation in embryonated chicken eggs. |
| first_indexed | 2025-11-15T14:10:27Z |
| format | Article |
| id | upm-111867 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T14:10:27Z |
| publishDate | 2002 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1118672025-03-03T06:21:50Z http://psasir.upm.edu.my/id/eprint/111867/ Novel peptides that inhibit the propagation of Newcastle disease virus Ramanujam, P. Tan, W. S. Nathan, S. Yusoff, K. A disulfide constrained random heptapeptide library displayed on filamentous bacteriophage M13 was applied to select specific ligands that interact with Newcastle disease virus (NDV). A fusion phage carrying the amino acid sequence TLTTKLY was selected from the panning procedure. An antibody competition assay showed that the selected phage was capable of competing with the polyclonal antibodies raised against NDV for binding sites on the virus. Determination of the binding affinity of this phage with NDV by an equilibrium binding assay in solution revealed two different dissociation constants, suggesting that there could be two distinct binding sites for the phage on NDV. Synthetic peptides with the sequence CTLTTKLYC, either in linear or cyclic conformations inhibited the binding of phage bearing the same sequence to NDV. These peptides also inhibited the hemolytic activity of the virus as well as its propagation in embryonated chicken eggs. Springer 2002 Article PeerReviewed Ramanujam, P. and Tan, W. S. and Nathan, S. and Yusoff, K. (2002) Novel peptides that inhibit the propagation of Newcastle disease virus. Archives of Virology, 147 (5). pp. 981-993. ISSN 0304-8608; eISSN: 1432-8798 https://link.springer.com/article/10.1007/s00705-001-0778-y?error=cookies_not_supported&code=4d674463-e51e-4a15-a6d0-a442fc159735 10.1007/s00705-001-0778-y |
| spellingShingle | Ramanujam, P. Tan, W. S. Nathan, S. Yusoff, K. Novel peptides that inhibit the propagation of Newcastle disease virus |
| title | Novel peptides that inhibit the propagation of Newcastle disease virus |
| title_full | Novel peptides that inhibit the propagation of Newcastle disease virus |
| title_fullStr | Novel peptides that inhibit the propagation of Newcastle disease virus |
| title_full_unstemmed | Novel peptides that inhibit the propagation of Newcastle disease virus |
| title_short | Novel peptides that inhibit the propagation of Newcastle disease virus |
| title_sort | novel peptides that inhibit the propagation of newcastle disease virus |
| url | http://psasir.upm.edu.my/id/eprint/111867/ http://psasir.upm.edu.my/id/eprint/111867/ http://psasir.upm.edu.my/id/eprint/111867/ |