Molecular characterization of Vibrio cholerae O1 outbreak strains in Miri, Sarawak (Malaysia)
Bacterial resistance to various antimicrobial agents is common in area with high usage of antibiotics. In this study, the data on antimicrobial susceptibility patterns of Vibrio cholerae O1 from patients during an outbreak period was found to be high but variable rates of multidrug resistance. Thirt...
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| Format: | Article |
| Language: | English |
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Elsevier
2002
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| Online Access: | http://psasir.upm.edu.my/id/eprint/111866/ http://psasir.upm.edu.my/id/eprint/111866/1/111866.pdf |
| _version_ | 1848865799617380352 |
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| author | Radu, Son Vincent, Micky Apun, Kasing Abdul Rahim, Raha Benjamin, Patrick Guda Yuherman, Unfound Rusul, Gulam |
| author_facet | Radu, Son Vincent, Micky Apun, Kasing Abdul Rahim, Raha Benjamin, Patrick Guda Yuherman, Unfound Rusul, Gulam |
| author_sort | Radu, Son |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Bacterial resistance to various antimicrobial agents is common in area with high usage of antibiotics. In this study, the data on antimicrobial susceptibility patterns of Vibrio cholerae O1 from patients during an outbreak period was found to be high but variable rates of multidrug resistance. Thirty-two of 33 V. cholerae isolates harboured the tcp, ctx, zot and ace genes, suggesting their possible roles in the outbreak cases. We analyzed the molecular diversity of a total of 33 strains of V. cholerae O1 isolated from 33 patients between November 1997 and April 1998 using random amplified polymorphic DNA (RAPD) analysis. The 30 typable isolates could be separated into four major clusters containing 5, 17, 2 and 6 isolates, respectively. However, no particular RAPD pattern was predictive of a particular pattern of antibiotic susceptibility. The findings of this study showed that multiple clones seemed to be responsible for cases in the outbreaks in the study area. |
| first_indexed | 2025-11-15T14:10:27Z |
| format | Article |
| id | upm-111866 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:10:27Z |
| publishDate | 2002 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1118662025-03-12T07:10:31Z http://psasir.upm.edu.my/id/eprint/111866/ Molecular characterization of Vibrio cholerae O1 outbreak strains in Miri, Sarawak (Malaysia) Radu, Son Vincent, Micky Apun, Kasing Abdul Rahim, Raha Benjamin, Patrick Guda Yuherman, Unfound Rusul, Gulam Bacterial resistance to various antimicrobial agents is common in area with high usage of antibiotics. In this study, the data on antimicrobial susceptibility patterns of Vibrio cholerae O1 from patients during an outbreak period was found to be high but variable rates of multidrug resistance. Thirty-two of 33 V. cholerae isolates harboured the tcp, ctx, zot and ace genes, suggesting their possible roles in the outbreak cases. We analyzed the molecular diversity of a total of 33 strains of V. cholerae O1 isolated from 33 patients between November 1997 and April 1998 using random amplified polymorphic DNA (RAPD) analysis. The 30 typable isolates could be separated into four major clusters containing 5, 17, 2 and 6 isolates, respectively. However, no particular RAPD pattern was predictive of a particular pattern of antibiotic susceptibility. The findings of this study showed that multiple clones seemed to be responsible for cases in the outbreaks in the study area. Elsevier 2002 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/111866/1/111866.pdf Radu, Son and Vincent, Micky and Apun, Kasing and Abdul Rahim, Raha and Benjamin, Patrick Guda and Yuherman, Unfound and Rusul, Gulam (2002) Molecular characterization of Vibrio cholerae O1 outbreak strains in Miri, Sarawak (Malaysia). Acta Tropica, 83 (2). pp. 169-176. ISSN 0001-706X https://linkinghub.elsevier.com/retrieve/pii/S0001706X02001109 10.1016/s0001-706x(02)00110-9 |
| spellingShingle | Radu, Son Vincent, Micky Apun, Kasing Abdul Rahim, Raha Benjamin, Patrick Guda Yuherman, Unfound Rusul, Gulam Molecular characterization of Vibrio cholerae O1 outbreak strains in Miri, Sarawak (Malaysia) |
| title | Molecular characterization of Vibrio cholerae O1 outbreak strains in Miri, Sarawak (Malaysia) |
| title_full | Molecular characterization of Vibrio cholerae O1 outbreak strains in Miri, Sarawak (Malaysia) |
| title_fullStr | Molecular characterization of Vibrio cholerae O1 outbreak strains in Miri, Sarawak (Malaysia) |
| title_full_unstemmed | Molecular characterization of Vibrio cholerae O1 outbreak strains in Miri, Sarawak (Malaysia) |
| title_short | Molecular characterization of Vibrio cholerae O1 outbreak strains in Miri, Sarawak (Malaysia) |
| title_sort | molecular characterization of vibrio cholerae o1 outbreak strains in miri, sarawak (malaysia) |
| url | http://psasir.upm.edu.my/id/eprint/111866/ http://psasir.upm.edu.my/id/eprint/111866/ http://psasir.upm.edu.my/id/eprint/111866/ http://psasir.upm.edu.my/id/eprint/111866/1/111866.pdf |