Expression of interleukin-18, interferon-γ and interleukin-10 in hepatocellular carcinoma
This is the first report on the detection of IL-18, IFN-γ and IL-10 proteins in hepatocelllular carcinoma. In the apparently normal surrounding tissue, 13 out of 17 paired specimens showed positive immunoreactivity to IL-18 (76.5%) compared with six out of 17 in the tumour portion (35.3% of specimen...
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| Format: | Article |
| Language: | English |
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Elsevier
2002
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| Online Access: | http://psasir.upm.edu.my/id/eprint/111865/ http://psasir.upm.edu.my/id/eprint/111865/3/111865.pdf |
| _version_ | 1848865799311196160 |
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| author | Chia, Chok Seng Ban, Kechen Ithnin, Hairuszah Singh, Harjit Krishnan, R Mokhtar, Suryati Malihan, Nik Seow, Heng Fong |
| author_facet | Chia, Chok Seng Ban, Kechen Ithnin, Hairuszah Singh, Harjit Krishnan, R Mokhtar, Suryati Malihan, Nik Seow, Heng Fong |
| author_sort | Chia, Chok Seng |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | This is the first report on the detection of IL-18, IFN-γ and IL-10 proteins in hepatocelllular carcinoma. In the apparently normal surrounding tissue, 13 out of 17 paired specimens showed positive immunoreactivity to IL-18 (76.5%) compared with six out of 17 in the tumour portion (35.3% of specimens). Thus, a significantly higher number of IL-18 positive specimens was found in the hepatocytes of apparently normal surrounding tissue compared with the tumour (P=0.018). In contrast, the number of specimens with positive immunoreactivity to the antibody against the Th1 cytokine, IFN-γ expression in the hepatocytes was lower. Only one specimen from the apparently normal surrounding tissue (one out of 17; 5.9%) and three other specimens from the tumour portion (three out of 17; 17.6%) had positive immunoreactivity. Similarly, the expression of the Th2 cytokine, IL-10 in normal (four out of 17; 23.5%) and tumour portions (five out of 17; 29.4%) was also low. Thus, there did not appear to be predominant Th2 immune response as denoted by IL-10 expression. Using the Spearman correlation rank test, a significant correlation between IL-18 expression in the apparently normal surrounding tissue and high α-foetoprotein (AFP) levels of >350 IU/l. No correlation between IL-18 expression in the tumour portion and clinicopathological factors was found. There was also no correlation found between IL-18 and the other cytokines, namely, IFN-γ and IL-10 expression These new findings provide additional information on the type of cytokines expressed in the tumour microenvironment and give a further insight into the role of cytokines in the pathogenesis of cancer which is critical for the development of effective immunotherapeutic approaches for cancer therapy in the future. |
| first_indexed | 2025-11-15T14:10:27Z |
| format | Article |
| id | upm-111865 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:10:27Z |
| publishDate | 2002 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1118652025-03-12T07:09:29Z http://psasir.upm.edu.my/id/eprint/111865/ Expression of interleukin-18, interferon-γ and interleukin-10 in hepatocellular carcinoma Chia, Chok Seng Ban, Kechen Ithnin, Hairuszah Singh, Harjit Krishnan, R Mokhtar, Suryati Malihan, Nik Seow, Heng Fong This is the first report on the detection of IL-18, IFN-γ and IL-10 proteins in hepatocelllular carcinoma. In the apparently normal surrounding tissue, 13 out of 17 paired specimens showed positive immunoreactivity to IL-18 (76.5%) compared with six out of 17 in the tumour portion (35.3% of specimens). Thus, a significantly higher number of IL-18 positive specimens was found in the hepatocytes of apparently normal surrounding tissue compared with the tumour (P=0.018). In contrast, the number of specimens with positive immunoreactivity to the antibody against the Th1 cytokine, IFN-γ expression in the hepatocytes was lower. Only one specimen from the apparently normal surrounding tissue (one out of 17; 5.9%) and three other specimens from the tumour portion (three out of 17; 17.6%) had positive immunoreactivity. Similarly, the expression of the Th2 cytokine, IL-10 in normal (four out of 17; 23.5%) and tumour portions (five out of 17; 29.4%) was also low. Thus, there did not appear to be predominant Th2 immune response as denoted by IL-10 expression. Using the Spearman correlation rank test, a significant correlation between IL-18 expression in the apparently normal surrounding tissue and high α-foetoprotein (AFP) levels of >350 IU/l. No correlation between IL-18 expression in the tumour portion and clinicopathological factors was found. There was also no correlation found between IL-18 and the other cytokines, namely, IFN-γ and IL-10 expression These new findings provide additional information on the type of cytokines expressed in the tumour microenvironment and give a further insight into the role of cytokines in the pathogenesis of cancer which is critical for the development of effective immunotherapeutic approaches for cancer therapy in the future. Elsevier 2002 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/111865/3/111865.pdf Chia, Chok Seng and Ban, Kechen and Ithnin, Hairuszah and Singh, Harjit and Krishnan, R and Mokhtar, Suryati and Malihan, Nik and Seow, Heng Fong (2002) Expression of interleukin-18, interferon-γ and interleukin-10 in hepatocellular carcinoma. Immunology Letters, 84 (3). pp. 163-172. ISSN 0165-2478 https://linkinghub.elsevier.com/retrieve/pii/S0165247802001761 10.1016/s0165-2478(02)00176-1 |
| spellingShingle | Chia, Chok Seng Ban, Kechen Ithnin, Hairuszah Singh, Harjit Krishnan, R Mokhtar, Suryati Malihan, Nik Seow, Heng Fong Expression of interleukin-18, interferon-γ and interleukin-10 in hepatocellular carcinoma |
| title | Expression of interleukin-18, interferon-γ and interleukin-10 in hepatocellular carcinoma |
| title_full | Expression of interleukin-18, interferon-γ and interleukin-10 in hepatocellular carcinoma |
| title_fullStr | Expression of interleukin-18, interferon-γ and interleukin-10 in hepatocellular carcinoma |
| title_full_unstemmed | Expression of interleukin-18, interferon-γ and interleukin-10 in hepatocellular carcinoma |
| title_short | Expression of interleukin-18, interferon-γ and interleukin-10 in hepatocellular carcinoma |
| title_sort | expression of interleukin-18, interferon-γ and interleukin-10 in hepatocellular carcinoma |
| url | http://psasir.upm.edu.my/id/eprint/111865/ http://psasir.upm.edu.my/id/eprint/111865/ http://psasir.upm.edu.my/id/eprint/111865/ http://psasir.upm.edu.my/id/eprint/111865/3/111865.pdf |