Lipase-catalyzed preparation, bioavailability and functional properties of a DHA-enriched tuna oil
Docosahexaenoic acid (DHA) in the form of triacylglycerols (TAG) are widely recognized for their health benefits. As naturally occurring DHA in the form of TAG are limited, this work developed and optimized a Candida rugosa lipase-catalyzed selective hydrolysis process to enrich DHA in tuna oil. The...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier BV
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/111525/ http://psasir.upm.edu.my/id/eprint/111525/1/1-s2.0-S0023643824006200-main.pdf |
| _version_ | 1848865711480373248 |
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| author | Yu, Jingwen Shen, Cai Chen, Haohui Luo, Min Zhang, Linshang Liu, Yanan Xu, Feng Tan, Chin-Ping Cheong, Ling-Zhi |
| author_facet | Yu, Jingwen Shen, Cai Chen, Haohui Luo, Min Zhang, Linshang Liu, Yanan Xu, Feng Tan, Chin-Ping Cheong, Ling-Zhi |
| author_sort | Yu, Jingwen |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Docosahexaenoic acid (DHA) in the form of triacylglycerols (TAG) are widely recognized for their health benefits. As naturally occurring DHA in the form of TAG are limited, this work developed and optimized a Candida rugosa lipase-catalyzed selective hydrolysis process to enrich DHA in tuna oil. The DHA-enriched tuna oil produced at optimal conditions (lipase concentration of 4 %, ratio of water to tuna oil of 1:3, reaction temperature of 55°C) contained 46.12 % of DHA, in which 55.24 % of DHA was located in sn-2 position of the glycerides. In-vitro bioavailability test shows, the DHA-enriched tuna oil (7.89 %) had significantly higher cellular uptake of DHA as compared to unprocessed oil. The DHA-enriched oil demonstrated increased intracellular oxidation and mitochondrial damage of Caco-2 cell. |
| first_indexed | 2025-11-15T14:09:03Z |
| format | Article |
| id | upm-111525 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:09:03Z |
| publishDate | 2024 |
| publisher | Elsevier BV |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1115252025-02-12T07:23:29Z http://psasir.upm.edu.my/id/eprint/111525/ Lipase-catalyzed preparation, bioavailability and functional properties of a DHA-enriched tuna oil Yu, Jingwen Shen, Cai Chen, Haohui Luo, Min Zhang, Linshang Liu, Yanan Xu, Feng Tan, Chin-Ping Cheong, Ling-Zhi Docosahexaenoic acid (DHA) in the form of triacylglycerols (TAG) are widely recognized for their health benefits. As naturally occurring DHA in the form of TAG are limited, this work developed and optimized a Candida rugosa lipase-catalyzed selective hydrolysis process to enrich DHA in tuna oil. The DHA-enriched tuna oil produced at optimal conditions (lipase concentration of 4 %, ratio of water to tuna oil of 1:3, reaction temperature of 55°C) contained 46.12 % of DHA, in which 55.24 % of DHA was located in sn-2 position of the glycerides. In-vitro bioavailability test shows, the DHA-enriched tuna oil (7.89 %) had significantly higher cellular uptake of DHA as compared to unprocessed oil. The DHA-enriched oil demonstrated increased intracellular oxidation and mitochondrial damage of Caco-2 cell. Elsevier BV 2024 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/111525/1/1-s2.0-S0023643824006200-main.pdf Yu, Jingwen and Shen, Cai and Chen, Haohui and Luo, Min and Zhang, Linshang and Liu, Yanan and Xu, Feng and Tan, Chin-Ping and Cheong, Ling-Zhi (2024) Lipase-catalyzed preparation, bioavailability and functional properties of a DHA-enriched tuna oil. LWT, 203. art. no. 116341. pp. 1-9. ISSN 0023-6438 https://linkinghub.elsevier.com/retrieve/pii/S0023643824006200 10.1016/j.lwt.2024.116341 |
| spellingShingle | Yu, Jingwen Shen, Cai Chen, Haohui Luo, Min Zhang, Linshang Liu, Yanan Xu, Feng Tan, Chin-Ping Cheong, Ling-Zhi Lipase-catalyzed preparation, bioavailability and functional properties of a DHA-enriched tuna oil |
| title | Lipase-catalyzed preparation, bioavailability and functional properties of a DHA-enriched tuna oil |
| title_full | Lipase-catalyzed preparation, bioavailability and functional properties of a DHA-enriched tuna oil |
| title_fullStr | Lipase-catalyzed preparation, bioavailability and functional properties of a DHA-enriched tuna oil |
| title_full_unstemmed | Lipase-catalyzed preparation, bioavailability and functional properties of a DHA-enriched tuna oil |
| title_short | Lipase-catalyzed preparation, bioavailability and functional properties of a DHA-enriched tuna oil |
| title_sort | lipase-catalyzed preparation, bioavailability and functional properties of a dha-enriched tuna oil |
| url | http://psasir.upm.edu.my/id/eprint/111525/ http://psasir.upm.edu.my/id/eprint/111525/ http://psasir.upm.edu.my/id/eprint/111525/ http://psasir.upm.edu.my/id/eprint/111525/1/1-s2.0-S0023643824006200-main.pdf |