Optimization of ionic liquid-based aqueous two-phase system for partial purification of bacteriocin-like inhibitory substance from Enterococcus faecium CC2 and its inhibitory activity against Streptococcus mutans
Background: Bacteriocin-like inhibitory substance (BLIS) demonstrates potent inhibitory effect against oral pathogens. To apply in oral care products, high purity of BLIS is required in view of the safety and effectiveness in the application. Therefore, aqueous two-phase systems (ATPS) are introduce...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/111337/ http://psasir.upm.edu.my/id/eprint/111337/1/1-s2.0-S1876107024000063-main.pdf |
| _version_ | 1848865659105050624 |
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| author | Ng, Zhang Jin Ng, Hui Suan Kee, Phei Er Gan, Chee-Yuen Ahmad Mokhtar, Ana Masara Abu Zarin, Mazni Lee, Chee Keong Tan, Joo Shun Lan, John Chi-Wei |
| author_facet | Ng, Zhang Jin Ng, Hui Suan Kee, Phei Er Gan, Chee-Yuen Ahmad Mokhtar, Ana Masara Abu Zarin, Mazni Lee, Chee Keong Tan, Joo Shun Lan, John Chi-Wei |
| author_sort | Ng, Zhang Jin |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Background: Bacteriocin-like inhibitory substance (BLIS) demonstrates potent inhibitory effect against oral pathogens. To apply in oral care products, high purity of BLIS is required in view of the safety and effectiveness in the application. Therefore, aqueous two-phase systems (ATPS) are introduced for the recovery and purification of BLIS. Methods: The optimization of ionic liquid-based ATPS (ILATPS) for the purification of BLIS from Enterococcus faecium CC2 was performed using response surface methodology (RSM). The partially purified BLIS was then identified, and the inhibitory effect against Streptococcus mutans UKMCC1019 was assessed. Significant Findings: ILATPS with a pH of 7 at a temperature of 40 °C, comprising of 10 % (w/w) fermentation broth, 15 % (w/w) 1‑butyl‑3-methylimidazolium tetrafluoroborate, (Bmim)BF4, 12.5 % (w/w) sodium citrate and 3 % (w/w) sodium chloride, resulted in the optimum purification factor of 4.406, recovery yield of 67.213 % and partition coefficient of 4.852. The BLIS was identified as enterocin CC2, with a molecular size of approximately 5.5–6 kDa. Furthermore, the partially purified BLIS at a concentration of 1.00 mg/mL demonstrated effective growth inhibition against S. mutans UKMCC1019. The ILATPS was shown to be a potential technology to enhance the purity of BLIS from E. faecium CC2. |
| first_indexed | 2025-11-15T14:08:13Z |
| format | Article |
| id | upm-111337 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:08:13Z |
| publishDate | 2024 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1113372024-07-10T07:53:30Z http://psasir.upm.edu.my/id/eprint/111337/ Optimization of ionic liquid-based aqueous two-phase system for partial purification of bacteriocin-like inhibitory substance from Enterococcus faecium CC2 and its inhibitory activity against Streptococcus mutans Ng, Zhang Jin Ng, Hui Suan Kee, Phei Er Gan, Chee-Yuen Ahmad Mokhtar, Ana Masara Abu Zarin, Mazni Lee, Chee Keong Tan, Joo Shun Lan, John Chi-Wei Background: Bacteriocin-like inhibitory substance (BLIS) demonstrates potent inhibitory effect against oral pathogens. To apply in oral care products, high purity of BLIS is required in view of the safety and effectiveness in the application. Therefore, aqueous two-phase systems (ATPS) are introduced for the recovery and purification of BLIS. Methods: The optimization of ionic liquid-based ATPS (ILATPS) for the purification of BLIS from Enterococcus faecium CC2 was performed using response surface methodology (RSM). The partially purified BLIS was then identified, and the inhibitory effect against Streptococcus mutans UKMCC1019 was assessed. Significant Findings: ILATPS with a pH of 7 at a temperature of 40 °C, comprising of 10 % (w/w) fermentation broth, 15 % (w/w) 1‑butyl‑3-methylimidazolium tetrafluoroborate, (Bmim)BF4, 12.5 % (w/w) sodium citrate and 3 % (w/w) sodium chloride, resulted in the optimum purification factor of 4.406, recovery yield of 67.213 % and partition coefficient of 4.852. The BLIS was identified as enterocin CC2, with a molecular size of approximately 5.5–6 kDa. Furthermore, the partially purified BLIS at a concentration of 1.00 mg/mL demonstrated effective growth inhibition against S. mutans UKMCC1019. The ILATPS was shown to be a potential technology to enhance the purity of BLIS from E. faecium CC2. Elsevier 2024 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/111337/1/1-s2.0-S1876107024000063-main.pdf Ng, Zhang Jin and Ng, Hui Suan and Kee, Phei Er and Gan, Chee-Yuen and Ahmad Mokhtar, Ana Masara and Abu Zarin, Mazni and Lee, Chee Keong and Tan, Joo Shun and Lan, John Chi-Wei (2024) Optimization of ionic liquid-based aqueous two-phase system for partial purification of bacteriocin-like inhibitory substance from Enterococcus faecium CC2 and its inhibitory activity against Streptococcus mutans. Journal of the Taiwan Institute of Chemical Engineers, 160. art. no. 105347. pp. 1-9. ISSN 1876-1070; ESSN: 1876-1089 https://linkinghub.elsevier.com/retrieve/pii/S1876107024000063 10.1016/j.jtice.2024.105347 |
| spellingShingle | Ng, Zhang Jin Ng, Hui Suan Kee, Phei Er Gan, Chee-Yuen Ahmad Mokhtar, Ana Masara Abu Zarin, Mazni Lee, Chee Keong Tan, Joo Shun Lan, John Chi-Wei Optimization of ionic liquid-based aqueous two-phase system for partial purification of bacteriocin-like inhibitory substance from Enterococcus faecium CC2 and its inhibitory activity against Streptococcus mutans |
| title | Optimization of ionic liquid-based aqueous two-phase system for partial purification of bacteriocin-like inhibitory substance from Enterococcus faecium CC2 and its inhibitory activity against Streptococcus mutans |
| title_full | Optimization of ionic liquid-based aqueous two-phase system for partial purification of bacteriocin-like inhibitory substance from Enterococcus faecium CC2 and its inhibitory activity against Streptococcus mutans |
| title_fullStr | Optimization of ionic liquid-based aqueous two-phase system for partial purification of bacteriocin-like inhibitory substance from Enterococcus faecium CC2 and its inhibitory activity against Streptococcus mutans |
| title_full_unstemmed | Optimization of ionic liquid-based aqueous two-phase system for partial purification of bacteriocin-like inhibitory substance from Enterococcus faecium CC2 and its inhibitory activity against Streptococcus mutans |
| title_short | Optimization of ionic liquid-based aqueous two-phase system for partial purification of bacteriocin-like inhibitory substance from Enterococcus faecium CC2 and its inhibitory activity against Streptococcus mutans |
| title_sort | optimization of ionic liquid-based aqueous two-phase system for partial purification of bacteriocin-like inhibitory substance from enterococcus faecium cc2 and its inhibitory activity against streptococcus mutans |
| url | http://psasir.upm.edu.my/id/eprint/111337/ http://psasir.upm.edu.my/id/eprint/111337/ http://psasir.upm.edu.my/id/eprint/111337/ http://psasir.upm.edu.my/id/eprint/111337/1/1-s2.0-S1876107024000063-main.pdf |