Expression of immuno-transcriptome response in red hybrid tilapia (Oreochromis sp.) hindgut following vaccination with feed-based bivalent vaccine
Streptococcosis and aeromoniasis are the main obstacles to sustainable tilapia production. Vaccination offered an effective method to control microbial infections. Previously, a feed-based bivalent vaccine (FBBV) containing killed whole organisms of Streptococcus agalactiae and Aeromonas hydrophila...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/111197/ http://psasir.upm.edu.my/id/eprint/111197/1/NSMA.pdf |
| _version_ | 1848865621465366528 |
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| author | Ali, Nur Shidaa Mohd Ngalimat, Mohamad Syazwan Saad, Mohd Zamri Azmai, Mohammad Noor Amal Salleh, Annas Zulperi, Zarirah Md Yasin, Ina Salwany |
| author_facet | Ali, Nur Shidaa Mohd Ngalimat, Mohamad Syazwan Saad, Mohd Zamri Azmai, Mohammad Noor Amal Salleh, Annas Zulperi, Zarirah Md Yasin, Ina Salwany |
| author_sort | Ali, Nur Shidaa Mohd |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Streptococcosis and aeromoniasis are the main obstacles to sustainable tilapia production. Vaccination offered an effective method to control microbial infections. Previously, a feed-based bivalent vaccine (FBBV) containing killed whole organisms of Streptococcus agalactiae and Aeromonas hydrophila mixed with 10% palm oil was successfully developed, which provided good protection against streptococcosis and aeromoniasis in Oreochromis sp. However, the mechanisms of immunities in vaccinated fish still need clarification. Here, the hindgut transcriptome of vaccinated and control fish was determined, as the gut displays higher affinity towards antigen uptake and nutrient absorption. The efficacy of FBBV to improve fish immunity was evaluated according to the expression of immune-related genes in the vaccinated fish hindgut throughout the 8-week experimental period using RT-qPCR. The vaccinated fish hindgut at week 6 was further subjected to transcriptomic analysis due to the high expression of immune-related genes and contained killed whole organisms. Results demonstrated the expression of immune-related genes was in correlation with the presence of killed whole organisms in the vaccinated fish hindgut. Transcriptomic analysis has allowed the prediction of robust immune-related pathways, including innate and adaptive immunological responses in vaccinated fish hindgut than control fish. Pathways related to the regulation of lipid metabolism and modulation of the immune system were also significantly enriched (p ≤.05). Overall, results offer a fundamental study on understanding the immunological response in Oreochromis sp. following vaccination with the FBBV pellet and support further application to prevent bacterial diseases in aquaculture. |
| first_indexed | 2025-11-15T14:07:37Z |
| format | Article |
| id | upm-111197 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:07:37Z |
| publishDate | 2024 |
| publisher | Wiley |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1111972024-07-10T07:51:40Z http://psasir.upm.edu.my/id/eprint/111197/ Expression of immuno-transcriptome response in red hybrid tilapia (Oreochromis sp.) hindgut following vaccination with feed-based bivalent vaccine Ali, Nur Shidaa Mohd Ngalimat, Mohamad Syazwan Saad, Mohd Zamri Azmai, Mohammad Noor Amal Salleh, Annas Zulperi, Zarirah Md Yasin, Ina Salwany Streptococcosis and aeromoniasis are the main obstacles to sustainable tilapia production. Vaccination offered an effective method to control microbial infections. Previously, a feed-based bivalent vaccine (FBBV) containing killed whole organisms of Streptococcus agalactiae and Aeromonas hydrophila mixed with 10% palm oil was successfully developed, which provided good protection against streptococcosis and aeromoniasis in Oreochromis sp. However, the mechanisms of immunities in vaccinated fish still need clarification. Here, the hindgut transcriptome of vaccinated and control fish was determined, as the gut displays higher affinity towards antigen uptake and nutrient absorption. The efficacy of FBBV to improve fish immunity was evaluated according to the expression of immune-related genes in the vaccinated fish hindgut throughout the 8-week experimental period using RT-qPCR. The vaccinated fish hindgut at week 6 was further subjected to transcriptomic analysis due to the high expression of immune-related genes and contained killed whole organisms. Results demonstrated the expression of immune-related genes was in correlation with the presence of killed whole organisms in the vaccinated fish hindgut. Transcriptomic analysis has allowed the prediction of robust immune-related pathways, including innate and adaptive immunological responses in vaccinated fish hindgut than control fish. Pathways related to the regulation of lipid metabolism and modulation of the immune system were also significantly enriched (p ≤.05). Overall, results offer a fundamental study on understanding the immunological response in Oreochromis sp. following vaccination with the FBBV pellet and support further application to prevent bacterial diseases in aquaculture. Wiley 2024 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/111197/1/NSMA.pdf Ali, Nur Shidaa Mohd and Ngalimat, Mohamad Syazwan and Saad, Mohd Zamri and Azmai, Mohammad Noor Amal and Salleh, Annas and Zulperi, Zarirah and Md Yasin, Ina Salwany (2024) Expression of immuno-transcriptome response in red hybrid tilapia (Oreochromis sp.) hindgut following vaccination with feed-based bivalent vaccine. Journal of Fish Diseases. pp. 1-17. ISSN 0140-7775; ESSN: 1365-2761 (In Press) https://onlinelibrary.wiley.com/doi/10.1111/jfd.13943 10.1111/jfd.13943 |
| spellingShingle | Ali, Nur Shidaa Mohd Ngalimat, Mohamad Syazwan Saad, Mohd Zamri Azmai, Mohammad Noor Amal Salleh, Annas Zulperi, Zarirah Md Yasin, Ina Salwany Expression of immuno-transcriptome response in red hybrid tilapia (Oreochromis sp.) hindgut following vaccination with feed-based bivalent vaccine |
| title | Expression of immuno-transcriptome response in red hybrid
tilapia (Oreochromis sp.) hindgut following vaccination with
feed-based bivalent vaccine |
| title_full | Expression of immuno-transcriptome response in red hybrid
tilapia (Oreochromis sp.) hindgut following vaccination with
feed-based bivalent vaccine |
| title_fullStr | Expression of immuno-transcriptome response in red hybrid
tilapia (Oreochromis sp.) hindgut following vaccination with
feed-based bivalent vaccine |
| title_full_unstemmed | Expression of immuno-transcriptome response in red hybrid
tilapia (Oreochromis sp.) hindgut following vaccination with
feed-based bivalent vaccine |
| title_short | Expression of immuno-transcriptome response in red hybrid
tilapia (Oreochromis sp.) hindgut following vaccination with
feed-based bivalent vaccine |
| title_sort | expression of immuno-transcriptome response in red hybrid
tilapia (oreochromis sp.) hindgut following vaccination with
feed-based bivalent vaccine |
| url | http://psasir.upm.edu.my/id/eprint/111197/ http://psasir.upm.edu.my/id/eprint/111197/ http://psasir.upm.edu.my/id/eprint/111197/ http://psasir.upm.edu.my/id/eprint/111197/1/NSMA.pdf |