In vivo temporal penetration of mesenchymal stem cell-derived exosome eye drops across ocular tissue
This study explores the potential application of extracellular vesicles (EVs) or exosomes derived from human mesenchymal stem cells (MSCs) in ophthalmology. Exosomes were isolated from MSCs’ culture supernatant, and applied as eye drops to rabbits’ eyes. Samples of aqueous (AH) and vitreous humour (...
| Main Authors: | , , , , , |
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| Format: | Article |
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Universiti Putra Malaysia Press
2023
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| Online Access: | http://psasir.upm.edu.my/id/eprint/110589/ |
| _version_ | 1848865558440706048 |
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| author | Moon, Nian Lim Qi, Hao Looi Koh, Benson Che’ Amat, Azlan Muhammad, Numan Khairuddin, Nurul Hayah |
| author_facet | Moon, Nian Lim Qi, Hao Looi Koh, Benson Che’ Amat, Azlan Muhammad, Numan Khairuddin, Nurul Hayah |
| author_sort | Moon, Nian Lim |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | This study explores the potential application of extracellular vesicles (EVs) or exosomes derived from human mesenchymal stem cells (MSCs) in ophthalmology. Exosomes were isolated from MSCs’ culture supernatant, and applied as eye drops to rabbits’ eyes. Samples of aqueous (AH) and vitreous humour (VH) were collected at different time points for exosomes marker analysis. Our results showed that CD63 was detected in all AH and VH samples. Whereas, for CD9 and CD81, despite minimally expressed in control samples, showed varying expression in VH samples at different time points, indicating penetration and retention of exosomes in the eyes’ structure. |
| first_indexed | 2025-11-15T14:06:37Z |
| format | Article |
| id | upm-110589 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T14:06:37Z |
| publishDate | 2023 |
| publisher | Universiti Putra Malaysia Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1105892024-06-13T02:54:01Z http://psasir.upm.edu.my/id/eprint/110589/ In vivo temporal penetration of mesenchymal stem cell-derived exosome eye drops across ocular tissue Moon, Nian Lim Qi, Hao Looi Koh, Benson Che’ Amat, Azlan Muhammad, Numan Khairuddin, Nurul Hayah This study explores the potential application of extracellular vesicles (EVs) or exosomes derived from human mesenchymal stem cells (MSCs) in ophthalmology. Exosomes were isolated from MSCs’ culture supernatant, and applied as eye drops to rabbits’ eyes. Samples of aqueous (AH) and vitreous humour (VH) were collected at different time points for exosomes marker analysis. Our results showed that CD63 was detected in all AH and VH samples. Whereas, for CD9 and CD81, despite minimally expressed in control samples, showed varying expression in VH samples at different time points, indicating penetration and retention of exosomes in the eyes’ structure. Universiti Putra Malaysia Press 2023-08 Article PeerReviewed Moon, Nian Lim and Qi, Hao Looi and Koh, Benson and Che’ Amat, Azlan and Muhammad, Numan and Khairuddin, Nurul Hayah (2023) In vivo temporal penetration of mesenchymal stem cell-derived exosome eye drops across ocular tissue. Malaysian Journal of Medicine and Health Sciences, 19 (suppl.8). pp. 25-26. ISSN 2636-9346 https://medic.upm.edu.my/upload/dokumen/20231115095038Complete_4th_MSCRT.pdf#page=28 |
| spellingShingle | Moon, Nian Lim Qi, Hao Looi Koh, Benson Che’ Amat, Azlan Muhammad, Numan Khairuddin, Nurul Hayah In vivo temporal penetration of mesenchymal stem cell-derived exosome eye drops across ocular tissue |
| title | In vivo temporal penetration of mesenchymal stem cell-derived exosome eye drops across ocular tissue |
| title_full | In vivo temporal penetration of mesenchymal stem cell-derived exosome eye drops across ocular tissue |
| title_fullStr | In vivo temporal penetration of mesenchymal stem cell-derived exosome eye drops across ocular tissue |
| title_full_unstemmed | In vivo temporal penetration of mesenchymal stem cell-derived exosome eye drops across ocular tissue |
| title_short | In vivo temporal penetration of mesenchymal stem cell-derived exosome eye drops across ocular tissue |
| title_sort | in vivo temporal penetration of mesenchymal stem cell-derived exosome eye drops across ocular tissue |
| url | http://psasir.upm.edu.my/id/eprint/110589/ http://psasir.upm.edu.my/id/eprint/110589/ |