Thermal inactivation D- and z-values of Salmonella Enteritidis and Salmonella Typhimurium in whole-muscle beef

Salmonella spp. is a significant foodborne pathogen present in raw meat products and in the processing environment. It can be eliminated by thermal processes such as cooking. Time and temperature in the thermal process play an important role in eliminating this pathogen. The objective of this study...

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Main Authors: Dora-Liyana, Abd Lataf, Mahyudin, Nor Ainy, Ismail-Fitry, Mohammad Rashedi, Ahmad Zainuri, Mohd Dzomir
Format: Article
Published: Malaysian Society of Applied Biology 2023
Online Access:http://psasir.upm.edu.my/id/eprint/110555/
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author Dora-Liyana, Abd Lataf
Mahyudin, Nor Ainy
Ismail-Fitry, Mohammad Rashedi
Ahmad Zainuri, Mohd Dzomir
author_facet Dora-Liyana, Abd Lataf
Mahyudin, Nor Ainy
Ismail-Fitry, Mohammad Rashedi
Ahmad Zainuri, Mohd Dzomir
author_sort Dora-Liyana, Abd Lataf
building UPM Institutional Repository
collection Online Access
description Salmonella spp. is a significant foodborne pathogen present in raw meat products and in the processing environment. It can be eliminated by thermal processes such as cooking. Time and temperature in the thermal process play an important role in eliminating this pathogen. The objective of this study is to determine the D- and z-values of Salmonella spp. in whole-muscle beef using the isothermal inactivation method in four temperatures at designated time intervals. Whole-muscle beef was inoculated with 7 to 8 log10 CFU/mL of Salmonella Typhimurium and Salmonella Enteritidis. The inoculated meat samples were heat treated at 58 °C, 60 °C, 62 °C, and 64 °C. At each temperature, Salmonella spp. survival rate was plotted and the D- and z-values were obtained by linear regression of the survival curve. The D-values for the thermal inactivation of whole-muscle beef are D58 °C=5.41 min, D60 °C=2.03 min, D62 °C=0.46 min, and D64 °C=0.18 min, while the z-value obtained was 3.94 °C. These findings will assist food processors in designing the critical limits on the critical control points of the cooking process that ensure safety against Salmonella spp. in cooked whole-muscle beef.
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institution Universiti Putra Malaysia
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publisher Malaysian Society of Applied Biology
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spelling upm-1105552024-05-27T08:41:03Z http://psasir.upm.edu.my/id/eprint/110555/ Thermal inactivation D- and z-values of Salmonella Enteritidis and Salmonella Typhimurium in whole-muscle beef Dora-Liyana, Abd Lataf Mahyudin, Nor Ainy Ismail-Fitry, Mohammad Rashedi Ahmad Zainuri, Mohd Dzomir Salmonella spp. is a significant foodborne pathogen present in raw meat products and in the processing environment. It can be eliminated by thermal processes such as cooking. Time and temperature in the thermal process play an important role in eliminating this pathogen. The objective of this study is to determine the D- and z-values of Salmonella spp. in whole-muscle beef using the isothermal inactivation method in four temperatures at designated time intervals. Whole-muscle beef was inoculated with 7 to 8 log10 CFU/mL of Salmonella Typhimurium and Salmonella Enteritidis. The inoculated meat samples were heat treated at 58 °C, 60 °C, 62 °C, and 64 °C. At each temperature, Salmonella spp. survival rate was plotted and the D- and z-values were obtained by linear regression of the survival curve. The D-values for the thermal inactivation of whole-muscle beef are D58 °C=5.41 min, D60 °C=2.03 min, D62 °C=0.46 min, and D64 °C=0.18 min, while the z-value obtained was 3.94 °C. These findings will assist food processors in designing the critical limits on the critical control points of the cooking process that ensure safety against Salmonella spp. in cooked whole-muscle beef. Malaysian Society of Applied Biology 2023 Article PeerReviewed Dora-Liyana, Abd Lataf and Mahyudin, Nor Ainy and Ismail-Fitry, Mohammad Rashedi and Ahmad Zainuri, Mohd Dzomir (2023) Thermal inactivation D- and z-values of Salmonella Enteritidis and Salmonella Typhimurium in whole-muscle beef. Malaysian Applied Biology, 52 (6). pp. 119-126. ISSN 0126-8643 https://jms.mabjournal.com/index.php/mab/article/view/2776 10.55230/mabjournal.v52i6.2776
spellingShingle Dora-Liyana, Abd Lataf
Mahyudin, Nor Ainy
Ismail-Fitry, Mohammad Rashedi
Ahmad Zainuri, Mohd Dzomir
Thermal inactivation D- and z-values of Salmonella Enteritidis and Salmonella Typhimurium in whole-muscle beef
title Thermal inactivation D- and z-values of Salmonella Enteritidis and Salmonella Typhimurium in whole-muscle beef
title_full Thermal inactivation D- and z-values of Salmonella Enteritidis and Salmonella Typhimurium in whole-muscle beef
title_fullStr Thermal inactivation D- and z-values of Salmonella Enteritidis and Salmonella Typhimurium in whole-muscle beef
title_full_unstemmed Thermal inactivation D- and z-values of Salmonella Enteritidis and Salmonella Typhimurium in whole-muscle beef
title_short Thermal inactivation D- and z-values of Salmonella Enteritidis and Salmonella Typhimurium in whole-muscle beef
title_sort thermal inactivation d- and z-values of salmonella enteritidis and salmonella typhimurium in whole-muscle beef
url http://psasir.upm.edu.my/id/eprint/110555/
http://psasir.upm.edu.my/id/eprint/110555/
http://psasir.upm.edu.my/id/eprint/110555/