Optimisation of phytate degradation in whole grain rice during germination processing using response surface methodology

Phytic acid (IP6), stored in seeds as metal salts known as phytates, binds to micronutrients and prevents its absorption by the human body. The germination process could improve cereal nutritional values by stimulating endogenous phytase activity and promoting phytate degradation. This study evaluat...

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Main Authors: Huei-Hong, Lee, Elisha, Yiu, Alvin-Lim-Teik, Zheng, Joseph-Choon-Fah, Bong, Su-Peng, Loh, Yiu, Pang Hung
Format: Article
Published: UNIMAS Publisher 2023
Online Access:http://psasir.upm.edu.my/id/eprint/110540/
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author Huei-Hong, Lee
Elisha, Yiu
Alvin-Lim-Teik, Zheng
Joseph-Choon-Fah, Bong
Su-Peng, Loh
Yiu, Pang Hung
author_facet Huei-Hong, Lee
Elisha, Yiu
Alvin-Lim-Teik, Zheng
Joseph-Choon-Fah, Bong
Su-Peng, Loh
Yiu, Pang Hung
author_sort Huei-Hong, Lee
building UPM Institutional Repository
collection Online Access
description Phytic acid (IP6), stored in seeds as metal salts known as phytates, binds to micronutrients and prevents its absorption by the human body. The germination process could improve cereal nutritional values by stimulating endogenous phytase activity and promoting phytate degradation. This study evaluated the physicochemical changes of phytates in rice cultivars with different IP6 contents, followed by optimisation of phytate degradation using response surface modeling. The magnitude of changes in IP6 content and phytase activity differed among rice cultivars. This suggested that the efficiency of germination treatments relied on the amount of natural phytic acid and phytase activity present in the rice grains. The cultivar “Tuan” was then selected and studied for the germination effect on phytate degradation using a central composite design. The cultivar gave a lower IP6 content, enhanced phytase activity and improved minerals bioaccessibility under acidic conditions. Acidic germination facilitated the degradation of phytate complexes in whole grain rice by making phytate complexes more soluble, accelerating phytase activity and thus, releasing mineral micronutrients from phytate globoids. The optimum germination condition was identified at pH 2.7, 25 °C over 12 h. In conclusion, germination processing facilitated phytate degradation in whole grain rice to make value-added rice products with low phytic acid and good mineral bioaccessibility.
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institution Universiti Putra Malaysia
institution_category Local University
last_indexed 2025-11-15T14:06:23Z
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publisher UNIMAS Publisher
recordtype eprints
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spelling upm-1105402024-05-15T23:41:23Z http://psasir.upm.edu.my/id/eprint/110540/ Optimisation of phytate degradation in whole grain rice during germination processing using response surface methodology Huei-Hong, Lee Elisha, Yiu Alvin-Lim-Teik, Zheng Joseph-Choon-Fah, Bong Su-Peng, Loh Yiu, Pang Hung Phytic acid (IP6), stored in seeds as metal salts known as phytates, binds to micronutrients and prevents its absorption by the human body. The germination process could improve cereal nutritional values by stimulating endogenous phytase activity and promoting phytate degradation. This study evaluated the physicochemical changes of phytates in rice cultivars with different IP6 contents, followed by optimisation of phytate degradation using response surface modeling. The magnitude of changes in IP6 content and phytase activity differed among rice cultivars. This suggested that the efficiency of germination treatments relied on the amount of natural phytic acid and phytase activity present in the rice grains. The cultivar “Tuan” was then selected and studied for the germination effect on phytate degradation using a central composite design. The cultivar gave a lower IP6 content, enhanced phytase activity and improved minerals bioaccessibility under acidic conditions. Acidic germination facilitated the degradation of phytate complexes in whole grain rice by making phytate complexes more soluble, accelerating phytase activity and thus, releasing mineral micronutrients from phytate globoids. The optimum germination condition was identified at pH 2.7, 25 °C over 12 h. In conclusion, germination processing facilitated phytate degradation in whole grain rice to make value-added rice products with low phytic acid and good mineral bioaccessibility. UNIMAS Publisher 2023 Article PeerReviewed Huei-Hong, Lee and Elisha, Yiu and Alvin-Lim-Teik, Zheng and Joseph-Choon-Fah, Bong and Su-Peng, Loh and Yiu, Pang Hung (2023) Optimisation of phytate degradation in whole grain rice during germination processing using response surface methodology. Borneo Journal of Resource Science and Technology, 13 (2). pp. 132-141. ISSN 2229-9769; ESSN: 0128-2972 https://publisher.unimas.my/ojs/index.php/BJRST/article/view/5347 10.33736/bjrst.5347.2023
spellingShingle Huei-Hong, Lee
Elisha, Yiu
Alvin-Lim-Teik, Zheng
Joseph-Choon-Fah, Bong
Su-Peng, Loh
Yiu, Pang Hung
Optimisation of phytate degradation in whole grain rice during germination processing using response surface methodology
title Optimisation of phytate degradation in whole grain rice during germination processing using response surface methodology
title_full Optimisation of phytate degradation in whole grain rice during germination processing using response surface methodology
title_fullStr Optimisation of phytate degradation in whole grain rice during germination processing using response surface methodology
title_full_unstemmed Optimisation of phytate degradation in whole grain rice during germination processing using response surface methodology
title_short Optimisation of phytate degradation in whole grain rice during germination processing using response surface methodology
title_sort optimisation of phytate degradation in whole grain rice during germination processing using response surface methodology
url http://psasir.upm.edu.my/id/eprint/110540/
http://psasir.upm.edu.my/id/eprint/110540/
http://psasir.upm.edu.my/id/eprint/110540/