Study of respiratory metabolism for multimetal tolerant bacteria under metallic stress
In aquatic environments, excessive amounts of inorganic nutrients, such as heavy metals, pose a metabolic risk and threaten to halt microbial activity. Metal-resistant bacteria, through various metabolic activities, can treat and detoxify harmful inorganic compounds. Thus, higher energy and electron...
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| Format: | Article |
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Department of Environmental Sciences, Arak University
2023
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| Online Access: | http://psasir.upm.edu.my/id/eprint/110499/ |
| _version_ | 1848865532082651136 |
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| author | Alhammadi, Eeman Halimoon, Normala Zulkeflee, Zufarzaana Wan Johari, Wan Lutfi |
| author_facet | Alhammadi, Eeman Halimoon, Normala Zulkeflee, Zufarzaana Wan Johari, Wan Lutfi |
| author_sort | Alhammadi, Eeman |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | In aquatic environments, excessive amounts of inorganic nutrients, such as heavy metals, pose a metabolic risk and threaten to halt microbial activity. Metal-resistant bacteria, through various metabolic activities, can treat and detoxify harmful inorganic compounds. Thus, higher energy and electron transport system (ETS) demands may be necessary for metal bioremediation, but cell viability may be affected. Therefore, the metabolic respiration activity of cells in the presence of Cu, Zn, Ni, and Cr individually and in the quaternary is an important aspect of this study that relies on estimating the action of respiration enzymes responsible for metabolic activity and glucose reduction over periods of metallic stress and growth phases. Three species of bacteria, including B. megaterium, S. ginsenosidimutans, and K. rhizophila, were isolated from the electroplating effluent and used to determine the activity level of catalase, dehydrogenase enzymes, and glucose reduction. Their ability to sustain metabolic activity and understand their role in conferring tolerance and bioremediation capabilities to bacteria was evaluated. The findings revealed that metabolic activity was greater during the exponential phase than during the stationary phase. Catalase production was less affected by high metal levels; additionally, sugar reduction was improved but decreased with increased metal levels, and growth progressed, in comparison to dehydrogenase activity, which was more sensitive to high metal levels. Although respiratory metabolism activity decreased with increasing cell age and high metal concentrations, metabolism and viability persisted under metallic stress. This establishes the tolerability of bacteria and recommends them for potential bioremediation of metallic pollutants and environmental clean-up. |
| first_indexed | 2025-11-15T14:06:12Z |
| format | Article |
| id | upm-110499 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T14:06:12Z |
| publishDate | 2023 |
| publisher | Department of Environmental Sciences, Arak University |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1104992024-06-15T07:53:29Z http://psasir.upm.edu.my/id/eprint/110499/ Study of respiratory metabolism for multimetal tolerant bacteria under metallic stress Alhammadi, Eeman Halimoon, Normala Zulkeflee, Zufarzaana Wan Johari, Wan Lutfi In aquatic environments, excessive amounts of inorganic nutrients, such as heavy metals, pose a metabolic risk and threaten to halt microbial activity. Metal-resistant bacteria, through various metabolic activities, can treat and detoxify harmful inorganic compounds. Thus, higher energy and electron transport system (ETS) demands may be necessary for metal bioremediation, but cell viability may be affected. Therefore, the metabolic respiration activity of cells in the presence of Cu, Zn, Ni, and Cr individually and in the quaternary is an important aspect of this study that relies on estimating the action of respiration enzymes responsible for metabolic activity and glucose reduction over periods of metallic stress and growth phases. Three species of bacteria, including B. megaterium, S. ginsenosidimutans, and K. rhizophila, were isolated from the electroplating effluent and used to determine the activity level of catalase, dehydrogenase enzymes, and glucose reduction. Their ability to sustain metabolic activity and understand their role in conferring tolerance and bioremediation capabilities to bacteria was evaluated. The findings revealed that metabolic activity was greater during the exponential phase than during the stationary phase. Catalase production was less affected by high metal levels; additionally, sugar reduction was improved but decreased with increased metal levels, and growth progressed, in comparison to dehydrogenase activity, which was more sensitive to high metal levels. Although respiratory metabolism activity decreased with increasing cell age and high metal concentrations, metabolism and viability persisted under metallic stress. This establishes the tolerability of bacteria and recommends them for potential bioremediation of metallic pollutants and environmental clean-up. Department of Environmental Sciences, Arak University 2023 Article PeerReviewed Alhammadi, Eeman and Halimoon, Normala and Zulkeflee, Zufarzaana and Wan Johari, Wan Lutfi (2023) Study of respiratory metabolism for multimetal tolerant bacteria under metallic stress. Journal of Wildlife and Biodiversity, 7 (spec.). pp. 547-575. ISSN 2588-3526 https://www.wildlife-biodiversity.com/index.php/jwb/article/view/530 10.5281/zenodo.10268356 |
| spellingShingle | Alhammadi, Eeman Halimoon, Normala Zulkeflee, Zufarzaana Wan Johari, Wan Lutfi Study of respiratory metabolism for multimetal tolerant bacteria under metallic stress |
| title | Study of respiratory metabolism for multimetal tolerant bacteria under metallic stress |
| title_full | Study of respiratory metabolism for multimetal tolerant bacteria under metallic stress |
| title_fullStr | Study of respiratory metabolism for multimetal tolerant bacteria under metallic stress |
| title_full_unstemmed | Study of respiratory metabolism for multimetal tolerant bacteria under metallic stress |
| title_short | Study of respiratory metabolism for multimetal tolerant bacteria under metallic stress |
| title_sort | study of respiratory metabolism for multimetal tolerant bacteria under metallic stress |
| url | http://psasir.upm.edu.my/id/eprint/110499/ http://psasir.upm.edu.my/id/eprint/110499/ http://psasir.upm.edu.my/id/eprint/110499/ |