Optimization of process conditions for the production of high-yield and high-quality Edible Bird's Nest (EBN) hydrolysate

Edible bird's nest (EBN) hydrolysate is widely used in EBN downstream products. This study aimed to optimize the process conditions (combination of heat treatment and enzymatic hydrolysis) to produce high-yield and high-quality EBN hydrolysate. The effects of four factors in the process were st...

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Main Authors: Yeo, Bee Hui, Wong, Shew Fung, Tan, Chin Ping, Rukayadi, Yaya, Lai, Oi Ming
Format: Article
Language:English
Published: Universiti Putra Malaysia 2023
Online Access:http://psasir.upm.edu.my/id/eprint/110431/
http://psasir.upm.edu.my/id/eprint/110431/1/110431.pdf
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author Yeo, Bee Hui
Wong, Shew Fung
Tan, Chin Ping
Rukayadi, Yaya
Lai, Oi Ming
author_facet Yeo, Bee Hui
Wong, Shew Fung
Tan, Chin Ping
Rukayadi, Yaya
Lai, Oi Ming
author_sort Yeo, Bee Hui
building UPM Institutional Repository
collection Online Access
description Edible bird's nest (EBN) hydrolysate is widely used in EBN downstream products. This study aimed to optimize the process conditions (combination of heat treatment and enzymatic hydrolysis) to produce high-yield and high-quality EBN hydrolysate. The effects of four factors in the process were studied by response surface methodology. The experimental factors are EBN temperature during double boiling (DB), DB duration, enzymatic hydrolysis duration, and the ratio of EBN to water. The recovery (yield) and quality (sialic acid [SA], 2,2-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and 2,2-diphenyl-1- picrylhydrazyl [DPPH]) of the final product were used as response variables. The Pearson correlation coefficient showed that: EBN temperature during DB affected product recovery (p < 0.01) and ABTS (p < 0.01), DB Duration affected DPPH (p < 0.01), and the ratio of EBN to water affected product recovery (p < 0.01). The duration of enzymatic hydrolysis was not significantly correlated with any of the responses and least significant factors in the model. Two optimal conditions for the processes obtained from this study were yield (product recovery) and quality. This study also showed that EBN hydrolysate produced from EBN by-products could be used as a nutraceutical because of the antioxidant activity and high SA content.
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spelling upm-1104312025-09-17T05:01:09Z http://psasir.upm.edu.my/id/eprint/110431/ Optimization of process conditions for the production of high-yield and high-quality Edible Bird's Nest (EBN) hydrolysate Yeo, Bee Hui Wong, Shew Fung Tan, Chin Ping Rukayadi, Yaya Lai, Oi Ming Edible bird's nest (EBN) hydrolysate is widely used in EBN downstream products. This study aimed to optimize the process conditions (combination of heat treatment and enzymatic hydrolysis) to produce high-yield and high-quality EBN hydrolysate. The effects of four factors in the process were studied by response surface methodology. The experimental factors are EBN temperature during double boiling (DB), DB duration, enzymatic hydrolysis duration, and the ratio of EBN to water. The recovery (yield) and quality (sialic acid [SA], 2,2-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and 2,2-diphenyl-1- picrylhydrazyl [DPPH]) of the final product were used as response variables. The Pearson correlation coefficient showed that: EBN temperature during DB affected product recovery (p < 0.01) and ABTS (p < 0.01), DB Duration affected DPPH (p < 0.01), and the ratio of EBN to water affected product recovery (p < 0.01). The duration of enzymatic hydrolysis was not significantly correlated with any of the responses and least significant factors in the model. Two optimal conditions for the processes obtained from this study were yield (product recovery) and quality. This study also showed that EBN hydrolysate produced from EBN by-products could be used as a nutraceutical because of the antioxidant activity and high SA content. Universiti Putra Malaysia 2023 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/110431/1/110431.pdf Yeo, Bee Hui and Wong, Shew Fung and Tan, Chin Ping and Rukayadi, Yaya and Lai, Oi Ming (2023) Optimization of process conditions for the production of high-yield and high-quality Edible Bird's Nest (EBN) hydrolysate. Pertanika Journal of Tropical Agricultural Science, 46 (3). pp. 931-950. ISSN 1511-3701; eISSN: 2231-8542 http://www.pertanika.upm.edu.my/pjtas/browse/regular-issue?article=JTAS-2666-2022 10.47836/pjtas.46.3.12
spellingShingle Yeo, Bee Hui
Wong, Shew Fung
Tan, Chin Ping
Rukayadi, Yaya
Lai, Oi Ming
Optimization of process conditions for the production of high-yield and high-quality Edible Bird's Nest (EBN) hydrolysate
title Optimization of process conditions for the production of high-yield and high-quality Edible Bird's Nest (EBN) hydrolysate
title_full Optimization of process conditions for the production of high-yield and high-quality Edible Bird's Nest (EBN) hydrolysate
title_fullStr Optimization of process conditions for the production of high-yield and high-quality Edible Bird's Nest (EBN) hydrolysate
title_full_unstemmed Optimization of process conditions for the production of high-yield and high-quality Edible Bird's Nest (EBN) hydrolysate
title_short Optimization of process conditions for the production of high-yield and high-quality Edible Bird's Nest (EBN) hydrolysate
title_sort optimization of process conditions for the production of high-yield and high-quality edible bird's nest (ebn) hydrolysate
url http://psasir.upm.edu.my/id/eprint/110431/
http://psasir.upm.edu.my/id/eprint/110431/
http://psasir.upm.edu.my/id/eprint/110431/
http://psasir.upm.edu.my/id/eprint/110431/1/110431.pdf