Mining the genome of Bacillus velezensis FS26 for probiotic markers and secondary metabolites with antimicrobial properties against aquaculture pathogens
Bacillus velezensis FS26 is a bacterium from the genus Bacillus that has been proven as a potential probiotic in aquaculture with a good antagonistic effect on Aeromonas spp. and Vibrio spp. Whole-genome sequencing (WGS) allows a comprehensive and in-depth analysis at the molecular level, and it is...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier BV
2023
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| Online Access: | http://psasir.upm.edu.my/id/eprint/110404/ http://psasir.upm.edu.my/id/eprint/110404/1/110404.pdf |
| _version_ | 1848865514952065024 |
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| author | Sam-on, Muhamad Firdaus Syahmi Mustafa, Shuhaimi Mohd Hashim, Amalia Yusof, Mohd Termizi Zulkifly, Shahrizim Malek, Ahmad Zuhairi Abdul Roslan, Muhamad Akhmal Hakim Mohd Asrore, Mohd Shaufi |
| author_facet | Sam-on, Muhamad Firdaus Syahmi Mustafa, Shuhaimi Mohd Hashim, Amalia Yusof, Mohd Termizi Zulkifly, Shahrizim Malek, Ahmad Zuhairi Abdul Roslan, Muhamad Akhmal Hakim Mohd Asrore, Mohd Shaufi |
| author_sort | Sam-on, Muhamad Firdaus Syahmi |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Bacillus velezensis FS26 is a bacterium from the genus Bacillus that has been proven as a potential probiotic in aquaculture with a good antagonistic effect on Aeromonas spp. and Vibrio spp. Whole-genome sequencing (WGS) allows a comprehensive and in-depth analysis at the molecular level, and it is becoming an increasingly significant technique in aquaculture research. Although numerous probiotic genomes have been sequenced and investigated recently, there are minimal data on in silico analysis of B. velezensis as a probiotic bacterium isolated from aquaculture sources. Thus, this study aims to analyse the general genome characteristics and probiotic markers from the B. velezensis FS26 genome with secondary metabolites predicted against aquaculture pathogens. The B. velezensis FS26 genome (GenBank Accession: JAOPEO000000000) assembly proved to be of high quality, with eight contigs containing 3,926,371 bp and an average G + C content of 46.5. According to antiSMASH analysis, five clusters of secondary metabolites from the B. velezensis FS26 genome showed 100 similarity. These clusters include Cluster 2 (bacilysin), Cluster 6 (bacillibactin), Cluster 7 (fengycin), Cluster 8 (bacillaene), and Cluster 9 (macrolactin H), which signify promising antibacterial, antifungal, and anticyanobacterial agents against pathogens in aquaculture. The probiotic markers of B. velezensis FS26 genome for adhesion capability in the hosts’ intestine, as well as the acid and bile salt-tolerant genes, were also detected through the Prokaryotic Genome Annotation System (Prokka) annotation pipeline. These results are in agreement with our previous in vitro data, suggesting that the in silico investigation facilitates establishing B. velezensis FS26 as a beneficial probiotic for use in aquaculture. |
| first_indexed | 2025-11-15T14:05:56Z |
| format | Article |
| id | upm-110404 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:05:56Z |
| publishDate | 2023 |
| publisher | Elsevier BV |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1104042024-10-08T06:18:44Z http://psasir.upm.edu.my/id/eprint/110404/ Mining the genome of Bacillus velezensis FS26 for probiotic markers and secondary metabolites with antimicrobial properties against aquaculture pathogens Sam-on, Muhamad Firdaus Syahmi Mustafa, Shuhaimi Mohd Hashim, Amalia Yusof, Mohd Termizi Zulkifly, Shahrizim Malek, Ahmad Zuhairi Abdul Roslan, Muhamad Akhmal Hakim Mohd Asrore, Mohd Shaufi Bacillus velezensis FS26 is a bacterium from the genus Bacillus that has been proven as a potential probiotic in aquaculture with a good antagonistic effect on Aeromonas spp. and Vibrio spp. Whole-genome sequencing (WGS) allows a comprehensive and in-depth analysis at the molecular level, and it is becoming an increasingly significant technique in aquaculture research. Although numerous probiotic genomes have been sequenced and investigated recently, there are minimal data on in silico analysis of B. velezensis as a probiotic bacterium isolated from aquaculture sources. Thus, this study aims to analyse the general genome characteristics and probiotic markers from the B. velezensis FS26 genome with secondary metabolites predicted against aquaculture pathogens. The B. velezensis FS26 genome (GenBank Accession: JAOPEO000000000) assembly proved to be of high quality, with eight contigs containing 3,926,371 bp and an average G + C content of 46.5. According to antiSMASH analysis, five clusters of secondary metabolites from the B. velezensis FS26 genome showed 100 similarity. These clusters include Cluster 2 (bacilysin), Cluster 6 (bacillibactin), Cluster 7 (fengycin), Cluster 8 (bacillaene), and Cluster 9 (macrolactin H), which signify promising antibacterial, antifungal, and anticyanobacterial agents against pathogens in aquaculture. The probiotic markers of B. velezensis FS26 genome for adhesion capability in the hosts’ intestine, as well as the acid and bile salt-tolerant genes, were also detected through the Prokaryotic Genome Annotation System (Prokka) annotation pipeline. These results are in agreement with our previous in vitro data, suggesting that the in silico investigation facilitates establishing B. velezensis FS26 as a beneficial probiotic for use in aquaculture. Elsevier BV 2023-08 Article NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/110404/1/110404.pdf Sam-on, Muhamad Firdaus Syahmi and Mustafa, Shuhaimi and Mohd Hashim, Amalia and Yusof, Mohd Termizi and Zulkifly, Shahrizim and Malek, Ahmad Zuhairi Abdul and Roslan, Muhamad Akhmal Hakim and Mohd Asrore, Mohd Shaufi (2023) Mining the genome of Bacillus velezensis FS26 for probiotic markers and secondary metabolites with antimicrobial properties against aquaculture pathogens. Microbial Pathogenesis, 181. art. no. 106161. pp. 1-7. ISSN 0882-4010 https://www.sciencedirect.com/science/article/pii/S0882401023001948 10.1016/j.micpath.2023.106161 |
| spellingShingle | Sam-on, Muhamad Firdaus Syahmi Mustafa, Shuhaimi Mohd Hashim, Amalia Yusof, Mohd Termizi Zulkifly, Shahrizim Malek, Ahmad Zuhairi Abdul Roslan, Muhamad Akhmal Hakim Mohd Asrore, Mohd Shaufi Mining the genome of Bacillus velezensis FS26 for probiotic markers and secondary metabolites with antimicrobial properties against aquaculture pathogens |
| title | Mining the genome of Bacillus velezensis FS26 for probiotic markers and secondary metabolites with antimicrobial properties against aquaculture pathogens |
| title_full | Mining the genome of Bacillus velezensis FS26 for probiotic markers and secondary metabolites with antimicrobial properties against aquaculture pathogens |
| title_fullStr | Mining the genome of Bacillus velezensis FS26 for probiotic markers and secondary metabolites with antimicrobial properties against aquaculture pathogens |
| title_full_unstemmed | Mining the genome of Bacillus velezensis FS26 for probiotic markers and secondary metabolites with antimicrobial properties against aquaculture pathogens |
| title_short | Mining the genome of Bacillus velezensis FS26 for probiotic markers and secondary metabolites with antimicrobial properties against aquaculture pathogens |
| title_sort | mining the genome of bacillus velezensis fs26 for probiotic markers and secondary metabolites with antimicrobial properties against aquaculture pathogens |
| url | http://psasir.upm.edu.my/id/eprint/110404/ http://psasir.upm.edu.my/id/eprint/110404/ http://psasir.upm.edu.my/id/eprint/110404/ http://psasir.upm.edu.my/id/eprint/110404/1/110404.pdf |