Performance of biomass and exopolysaccharide production from the medicinal mushroom Ganoderma lucidum in a new fabricated Air-L-Shaped Bioreactor (ALSB)
Conventional stirred-tank bioreactor (STR) designs are optimised for cultures of bacteria but not fungal cultures; therefore, a new Air-L-Shaped Bioreactor (ALSB) was fabricated. The ALSB was designed to eliminate the wall growth and clumping of fungal mycelium in STRs. Ganoderma lucidum was used as...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Multidisciplinary Digital Publishing Institute (MDPI)
2023
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| Online Access: | http://psasir.upm.edu.my/id/eprint/110363/ http://psasir.upm.edu.my/id/eprint/110363/1/110363.pdf |
| _version_ | 1848865505995128832 |
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| author | Supramani, Sugenendran Rejab, Nur Ardiyana Ilham, Zul Ahmad, Rahayu Show, Pau-Loke Ibrahim, Mohamad Faizal Wan Mohtar, Wan Abd Al Qadr Imad |
| author_facet | Supramani, Sugenendran Rejab, Nur Ardiyana Ilham, Zul Ahmad, Rahayu Show, Pau-Loke Ibrahim, Mohamad Faizal Wan Mohtar, Wan Abd Al Qadr Imad |
| author_sort | Supramani, Sugenendran |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Conventional stirred-tank bioreactor (STR) designs are optimised for cultures of bacteria but not fungal cultures; therefore, a new Air-L-Shaped Bioreactor (ALSB) was fabricated. The ALSB was designed to eliminate the wall growth and clumping of fungal mycelium in STRs. Ganoderma lucidum was used as a fungal model and its biomass and exopolysaccharide (EPS) production were maximised by optimising the agitation rate, glucose concentration, initial pH, and aeration via response surface methodology (RSM). The ALSB system generated 7.8 g/L of biomass (biomass optimised conditions: 110 rpm, 24 g/L glucose, pH 5.6, and 3 v/v of aeration) and 4.4 g/L of EPS (EPS optimised conditions: 90 rpm, 30 g/L glucose, pH 4, and 2.5 v/v of aeration). In combination, for both optimised conditions, biomass (7.9 g/L) and EPS (4.6 g/L) were produced at 110 rpm, 30 g/L glucose, pH 4, and 3 v/v of aeration with minimal wall growth. The data prove that the ALSB is a blueprint for efficient economical fungal cultivation. |
| first_indexed | 2025-11-15T14:05:47Z |
| format | Article |
| id | upm-110363 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:05:47Z |
| publishDate | 2023 |
| publisher | Multidisciplinary Digital Publishing Institute (MDPI) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1103632024-10-09T02:21:03Z http://psasir.upm.edu.my/id/eprint/110363/ Performance of biomass and exopolysaccharide production from the medicinal mushroom Ganoderma lucidum in a new fabricated Air-L-Shaped Bioreactor (ALSB) Supramani, Sugenendran Rejab, Nur Ardiyana Ilham, Zul Ahmad, Rahayu Show, Pau-Loke Ibrahim, Mohamad Faizal Wan Mohtar, Wan Abd Al Qadr Imad Conventional stirred-tank bioreactor (STR) designs are optimised for cultures of bacteria but not fungal cultures; therefore, a new Air-L-Shaped Bioreactor (ALSB) was fabricated. The ALSB was designed to eliminate the wall growth and clumping of fungal mycelium in STRs. Ganoderma lucidum was used as a fungal model and its biomass and exopolysaccharide (EPS) production were maximised by optimising the agitation rate, glucose concentration, initial pH, and aeration via response surface methodology (RSM). The ALSB system generated 7.8 g/L of biomass (biomass optimised conditions: 110 rpm, 24 g/L glucose, pH 5.6, and 3 v/v of aeration) and 4.4 g/L of EPS (EPS optimised conditions: 90 rpm, 30 g/L glucose, pH 4, and 2.5 v/v of aeration). In combination, for both optimised conditions, biomass (7.9 g/L) and EPS (4.6 g/L) were produced at 110 rpm, 30 g/L glucose, pH 4, and 3 v/v of aeration with minimal wall growth. The data prove that the ALSB is a blueprint for efficient economical fungal cultivation. Multidisciplinary Digital Publishing Institute (MDPI) 2023 Article PeerReviewed text en cc_by_4 http://psasir.upm.edu.my/id/eprint/110363/1/110363.pdf Supramani, Sugenendran and Rejab, Nur Ardiyana and Ilham, Zul and Ahmad, Rahayu and Show, Pau-Loke and Ibrahim, Mohamad Faizal and Wan Mohtar, Wan Abd Al Qadr Imad (2023) Performance of biomass and exopolysaccharide production from the medicinal mushroom Ganoderma lucidum in a new fabricated Air-L-Shaped Bioreactor (ALSB). Processes, 11 (670). pp. 1-14. ISSN 2227-9717 https://www.mdpi.com/2227-9717/11/3/670 10.3390/pr11030670 |
| spellingShingle | Supramani, Sugenendran Rejab, Nur Ardiyana Ilham, Zul Ahmad, Rahayu Show, Pau-Loke Ibrahim, Mohamad Faizal Wan Mohtar, Wan Abd Al Qadr Imad Performance of biomass and exopolysaccharide production from the medicinal mushroom Ganoderma lucidum in a new fabricated Air-L-Shaped Bioreactor (ALSB) |
| title | Performance of biomass and exopolysaccharide production from the medicinal mushroom Ganoderma lucidum in a new fabricated Air-L-Shaped Bioreactor (ALSB) |
| title_full | Performance of biomass and exopolysaccharide production from the medicinal mushroom Ganoderma lucidum in a new fabricated Air-L-Shaped Bioreactor (ALSB) |
| title_fullStr | Performance of biomass and exopolysaccharide production from the medicinal mushroom Ganoderma lucidum in a new fabricated Air-L-Shaped Bioreactor (ALSB) |
| title_full_unstemmed | Performance of biomass and exopolysaccharide production from the medicinal mushroom Ganoderma lucidum in a new fabricated Air-L-Shaped Bioreactor (ALSB) |
| title_short | Performance of biomass and exopolysaccharide production from the medicinal mushroom Ganoderma lucidum in a new fabricated Air-L-Shaped Bioreactor (ALSB) |
| title_sort | performance of biomass and exopolysaccharide production from the medicinal mushroom ganoderma lucidum in a new fabricated air-l-shaped bioreactor (alsb) |
| url | http://psasir.upm.edu.my/id/eprint/110363/ http://psasir.upm.edu.my/id/eprint/110363/ http://psasir.upm.edu.my/id/eprint/110363/ http://psasir.upm.edu.my/id/eprint/110363/1/110363.pdf |