Subcritical water extraction of total phenolic compounds from Piper betle L. leaves: effect of process conditions and characterization
Subcritical water extraction (SWE) as a green separation technology becomes a suitable choice to recover the phenolic compounds of Piper betle L. (PBL) leaves. This experiment utilized subcritical water using a batch fluid extraction system with two factors of process conditions: temperature ranging...
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| Format: | Article |
| Language: | English |
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Springer
2023
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| Online Access: | http://psasir.upm.edu.my/id/eprint/110347/ http://psasir.upm.edu.my/id/eprint/110347/1/110347.pdf |
| _version_ | 1848865501068918784 |
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| author | Rahmah, Nur Lailatul Mustapa Kamal, Siti Mazlina Sulaiman, Alifdalino Taip, Farah Saleena Siajam, Shamsul Izhar |
| author_facet | Rahmah, Nur Lailatul Mustapa Kamal, Siti Mazlina Sulaiman, Alifdalino Taip, Farah Saleena Siajam, Shamsul Izhar |
| author_sort | Rahmah, Nur Lailatul |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Subcritical water extraction (SWE) as a green separation technology becomes a suitable choice to recover the phenolic compounds of Piper betle L. (PBL) leaves. This experiment utilized subcritical water using a batch fluid extraction system with two factors of process conditions: temperature ranging from 100 to 275 °C and time from 5 to 30 min. Positive interaction effect (p < 0.05) between temperature and time for total phenolic content (TPC) and antioxidant activity (AA) were obtained from the two-way analysis of variance. The highest TPC (7.89 ± 0.26 mg GAE/g) and AA (92.98 ± 1.79%) were obtained by subcritical water at the operation conditions of 200 °C, 1.55 MPa, 15% solid loading for 5 min. The functional groups detected from infra-red spectra analysis were presumably the phenolic compound’s structure. Scanning electron microscope showed pores formation in the residue of PBL leaves after treated by SWE. The best extract of PBL leaves recovered from the SWE process inhibited the proliferation of the SW-480 (human colorectal adenocarcinoma) cell with IC50 value of 100 µg/mL. Hence, these findings enhance the understanding of SWE as a good alternative method for separating phenolic compounds from PBL leaves, and the best extract was potentially applicable as an anticancer treatment. |
| first_indexed | 2025-11-15T14:05:42Z |
| format | Article |
| id | upm-110347 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:05:42Z |
| publishDate | 2023 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1103472025-06-23T02:20:06Z http://psasir.upm.edu.my/id/eprint/110347/ Subcritical water extraction of total phenolic compounds from Piper betle L. leaves: effect of process conditions and characterization Rahmah, Nur Lailatul Mustapa Kamal, Siti Mazlina Sulaiman, Alifdalino Taip, Farah Saleena Siajam, Shamsul Izhar Subcritical water extraction (SWE) as a green separation technology becomes a suitable choice to recover the phenolic compounds of Piper betle L. (PBL) leaves. This experiment utilized subcritical water using a batch fluid extraction system with two factors of process conditions: temperature ranging from 100 to 275 °C and time from 5 to 30 min. Positive interaction effect (p < 0.05) between temperature and time for total phenolic content (TPC) and antioxidant activity (AA) were obtained from the two-way analysis of variance. The highest TPC (7.89 ± 0.26 mg GAE/g) and AA (92.98 ± 1.79%) were obtained by subcritical water at the operation conditions of 200 °C, 1.55 MPa, 15% solid loading for 5 min. The functional groups detected from infra-red spectra analysis were presumably the phenolic compound’s structure. Scanning electron microscope showed pores formation in the residue of PBL leaves after treated by SWE. The best extract of PBL leaves recovered from the SWE process inhibited the proliferation of the SW-480 (human colorectal adenocarcinoma) cell with IC50 value of 100 µg/mL. Hence, these findings enhance the understanding of SWE as a good alternative method for separating phenolic compounds from PBL leaves, and the best extract was potentially applicable as an anticancer treatment. Springer 2023-07 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/110347/1/110347.pdf Rahmah, Nur Lailatul and Mustapa Kamal, Siti Mazlina and Sulaiman, Alifdalino and Taip, Farah Saleena and Siajam, Shamsul Izhar (2023) Subcritical water extraction of total phenolic compounds from Piper betle L. leaves: effect of process conditions and characterization. Journal of Food Measurement and Characterization, 17 (6). pp. 5606-5618. ISSN 2193-4126; eISSN: 2193-4134 https://link.springer.com/article/10.1007/s11694-023-02068-3?error=cookies_not_supported&code=3b663e20-431d-4f53-82cf-2917de79a833 10.1007/s11694-023-02068-3 |
| spellingShingle | Rahmah, Nur Lailatul Mustapa Kamal, Siti Mazlina Sulaiman, Alifdalino Taip, Farah Saleena Siajam, Shamsul Izhar Subcritical water extraction of total phenolic compounds from Piper betle L. leaves: effect of process conditions and characterization |
| title | Subcritical water extraction of total phenolic compounds from Piper betle L. leaves: effect of process conditions and characterization |
| title_full | Subcritical water extraction of total phenolic compounds from Piper betle L. leaves: effect of process conditions and characterization |
| title_fullStr | Subcritical water extraction of total phenolic compounds from Piper betle L. leaves: effect of process conditions and characterization |
| title_full_unstemmed | Subcritical water extraction of total phenolic compounds from Piper betle L. leaves: effect of process conditions and characterization |
| title_short | Subcritical water extraction of total phenolic compounds from Piper betle L. leaves: effect of process conditions and characterization |
| title_sort | subcritical water extraction of total phenolic compounds from piper betle l. leaves: effect of process conditions and characterization |
| url | http://psasir.upm.edu.my/id/eprint/110347/ http://psasir.upm.edu.my/id/eprint/110347/ http://psasir.upm.edu.my/id/eprint/110347/ http://psasir.upm.edu.my/id/eprint/110347/1/110347.pdf |