Anti‑Diabetic Effect of Lactobacillus Paracasei Isolated from Malaysian Water Kefir Grains
The prevalence of type 2 diabetes mellitus (T2DM) is alarming because it is always linked to the increase in chronic diseases, mortality, and socioeconomic burden. Water kefir has a wide range of functional and probiotic characteristics attributed to the microorganisms present in the kefir grains. T...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Published: |
Springer Nature
2023
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/110301/ |
| _version_ | 1848865488221765632 |
|---|---|
| author | Talib, Noorshafadzilah Mohamad, Nurul Elyani Yeap, Swee Keong Ho, Chai Ling Masarudin, Mas Jaffri Abd-Aziz, Suraini Izham, Mira Nadiah Mohd Kumar, Muganti Rajah Hussin, Yazmin Alitheen, Noorjahan Banu |
| author_facet | Talib, Noorshafadzilah Mohamad, Nurul Elyani Yeap, Swee Keong Ho, Chai Ling Masarudin, Mas Jaffri Abd-Aziz, Suraini Izham, Mira Nadiah Mohd Kumar, Muganti Rajah Hussin, Yazmin Alitheen, Noorjahan Banu |
| author_sort | Talib, Noorshafadzilah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The prevalence of type 2 diabetes mellitus (T2DM) is alarming because it is always linked to the increase in chronic diseases, mortality, and socioeconomic burden. Water kefir has a wide range of functional and probiotic characteristics attributed to the microorganisms present in the kefir grains. The present study aims to evaluate the in vivo anti-diabetic potential of the isolated Lactobacillus paracasei from Malaysian water kefir grains (MWKG) which was reported to have excellent probiotic properties and high antioxidant activities as reported previously. High-fat diet/streptozotocin (HFD/STZ) induction was used to obtain a T2DM model followed by treatment with the isolated L. paracasei from MWKG. The levels of glucose, insulin, and in vivo liver antioxidants were quantified after 14 weeks. Gene expression analysis of the liver was also carried out using microarray analysis, and several genes were selected for validation using quantitative real-time PCR. Insulin tolerance test demonstrated that the L. paracasei isolated from the MWKG alleviated T2DM by improving the area under the curve of the insulin tolerance test whereby low-dose and high-dose concentrations treated groups showed 2424.50±437.02 mmol/L·min and 2017.50±347.09 mmol/L·min, respectively, compared to untreated diabetic mice which was 3884.50±39.36 mmol/ L·min. Additionally, treatment with the isolated L. paracasei from MWKG regulated the expression of several genes related to glucose homeostasis and lipid metabolism in diabetic mice. These results suggested that the isolated L. paracasei from MWKG could be a potential dietary supplement for T2DM. |
| first_indexed | 2025-11-15T14:05:30Z |
| format | Article |
| id | upm-110301 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T14:05:30Z |
| publishDate | 2023 |
| publisher | Springer Nature |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1103012024-09-03T03:00:10Z http://psasir.upm.edu.my/id/eprint/110301/ Anti‑Diabetic Effect of Lactobacillus Paracasei Isolated from Malaysian Water Kefir Grains Talib, Noorshafadzilah Mohamad, Nurul Elyani Yeap, Swee Keong Ho, Chai Ling Masarudin, Mas Jaffri Abd-Aziz, Suraini Izham, Mira Nadiah Mohd Kumar, Muganti Rajah Hussin, Yazmin Alitheen, Noorjahan Banu The prevalence of type 2 diabetes mellitus (T2DM) is alarming because it is always linked to the increase in chronic diseases, mortality, and socioeconomic burden. Water kefir has a wide range of functional and probiotic characteristics attributed to the microorganisms present in the kefir grains. The present study aims to evaluate the in vivo anti-diabetic potential of the isolated Lactobacillus paracasei from Malaysian water kefir grains (MWKG) which was reported to have excellent probiotic properties and high antioxidant activities as reported previously. High-fat diet/streptozotocin (HFD/STZ) induction was used to obtain a T2DM model followed by treatment with the isolated L. paracasei from MWKG. The levels of glucose, insulin, and in vivo liver antioxidants were quantified after 14 weeks. Gene expression analysis of the liver was also carried out using microarray analysis, and several genes were selected for validation using quantitative real-time PCR. Insulin tolerance test demonstrated that the L. paracasei isolated from the MWKG alleviated T2DM by improving the area under the curve of the insulin tolerance test whereby low-dose and high-dose concentrations treated groups showed 2424.50±437.02 mmol/L·min and 2017.50±347.09 mmol/L·min, respectively, compared to untreated diabetic mice which was 3884.50±39.36 mmol/ L·min. Additionally, treatment with the isolated L. paracasei from MWKG regulated the expression of several genes related to glucose homeostasis and lipid metabolism in diabetic mice. These results suggested that the isolated L. paracasei from MWKG could be a potential dietary supplement for T2DM. Springer Nature 2023-09 Article NonPeerReviewed Talib, Noorshafadzilah and Mohamad, Nurul Elyani and Yeap, Swee Keong and Ho, Chai Ling and Masarudin, Mas Jaffri and Abd-Aziz, Suraini and Izham, Mira Nadiah Mohd and Kumar, Muganti Rajah and Hussin, Yazmin and Alitheen, Noorjahan Banu (2023) Anti‑Diabetic Effect of Lactobacillus Paracasei Isolated from Malaysian Water Kefir Grains. Probiotics and Antimicrobial Proteins. pp. 1-20. ISSN 1867-1306 https://link.springer.com/article/10.1007/s12602-023-10159-2 10.1007/s12602-023-10159-2 |
| spellingShingle | Talib, Noorshafadzilah Mohamad, Nurul Elyani Yeap, Swee Keong Ho, Chai Ling Masarudin, Mas Jaffri Abd-Aziz, Suraini Izham, Mira Nadiah Mohd Kumar, Muganti Rajah Hussin, Yazmin Alitheen, Noorjahan Banu Anti‑Diabetic Effect of Lactobacillus Paracasei Isolated from Malaysian Water Kefir Grains |
| title | Anti‑Diabetic Effect of Lactobacillus Paracasei Isolated from Malaysian Water Kefir Grains |
| title_full | Anti‑Diabetic Effect of Lactobacillus Paracasei Isolated from Malaysian Water Kefir Grains |
| title_fullStr | Anti‑Diabetic Effect of Lactobacillus Paracasei Isolated from Malaysian Water Kefir Grains |
| title_full_unstemmed | Anti‑Diabetic Effect of Lactobacillus Paracasei Isolated from Malaysian Water Kefir Grains |
| title_short | Anti‑Diabetic Effect of Lactobacillus Paracasei Isolated from Malaysian Water Kefir Grains |
| title_sort | anti‑diabetic effect of lactobacillus paracasei isolated from malaysian water kefir grains |
| url | http://psasir.upm.edu.my/id/eprint/110301/ http://psasir.upm.edu.my/id/eprint/110301/ http://psasir.upm.edu.my/id/eprint/110301/ |