In Vitro blood-brain barrier models for neuroinfectious diseases: a narrative review
The blood-brain barrier (BBB) is a complex, dynamic, and adaptable barrier between the peripheral blood system and the central nervous system. While this barrier protects the brain and spinal cord from inflammation and infection, it prevents most drugs from reaching the brain tissue. With the expand...
| Main Authors: | , , , , , , |
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| Format: | Article |
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Bentham Science Publishers
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/110260/ |
| _version_ | 1848865476404314112 |
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| author | Badawi, Ahmad Hussein Mohamad, Nur Afiqah Stanslas, Johnson Kirby, Brian Patrick Neela, Vasantha Kumari Ramasamy, Rajesh Basri, Hamidon |
| author_facet | Badawi, Ahmad Hussein Mohamad, Nur Afiqah Stanslas, Johnson Kirby, Brian Patrick Neela, Vasantha Kumari Ramasamy, Rajesh Basri, Hamidon |
| author_sort | Badawi, Ahmad Hussein |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The blood-brain barrier (BBB) is a complex, dynamic, and adaptable barrier between the peripheral blood system and the central nervous system. While this barrier protects the brain and spinal cord from inflammation and infection, it prevents most drugs from reaching the brain tissue. With the expanding interest in the pathophysiology of BBB, the development of in vitro BBB models has dramatically evolved. However, due to the lack of a standard model, a range of experimental protocols, BBB-phenotype markers, and permeability flux markers was utilized to construct in vitro BBB models. Several neuroinfectious diseases are associated with BBB dysfunction. To conduct neuroinfectious disease research effectively, there stems a need to design representative in vitro human BBB models that mimic the BBB's functional and molecular properties. The highest necessity is for an in vitro standardised BBB model that accurately represents all the complexities of an intact brain barrier. Thus, this in-depth review aims to describe the optimization and validation parameters for building BBB models and to discuss previous research on neuroinfectious diseases that have utilized in vitro BBB models. The findings in this review may serve as a basis for more efficient optimisation, validation, and maintenance of a structurally and functionally intact BBB model, particularly for future studies on neuroinfectious diseases. |
| first_indexed | 2025-11-15T14:05:19Z |
| format | Article |
| id | upm-110260 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T14:05:19Z |
| publishDate | 2024 |
| publisher | Bentham Science Publishers |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1102602024-06-26T05:51:30Z http://psasir.upm.edu.my/id/eprint/110260/ In Vitro blood-brain barrier models for neuroinfectious diseases: a narrative review Badawi, Ahmad Hussein Mohamad, Nur Afiqah Stanslas, Johnson Kirby, Brian Patrick Neela, Vasantha Kumari Ramasamy, Rajesh Basri, Hamidon The blood-brain barrier (BBB) is a complex, dynamic, and adaptable barrier between the peripheral blood system and the central nervous system. While this barrier protects the brain and spinal cord from inflammation and infection, it prevents most drugs from reaching the brain tissue. With the expanding interest in the pathophysiology of BBB, the development of in vitro BBB models has dramatically evolved. However, due to the lack of a standard model, a range of experimental protocols, BBB-phenotype markers, and permeability flux markers was utilized to construct in vitro BBB models. Several neuroinfectious diseases are associated with BBB dysfunction. To conduct neuroinfectious disease research effectively, there stems a need to design representative in vitro human BBB models that mimic the BBB's functional and molecular properties. The highest necessity is for an in vitro standardised BBB model that accurately represents all the complexities of an intact brain barrier. Thus, this in-depth review aims to describe the optimization and validation parameters for building BBB models and to discuss previous research on neuroinfectious diseases that have utilized in vitro BBB models. The findings in this review may serve as a basis for more efficient optimisation, validation, and maintenance of a structurally and functionally intact BBB model, particularly for future studies on neuroinfectious diseases. Bentham Science Publishers 2024 Article PeerReviewed Badawi, Ahmad Hussein and Mohamad, Nur Afiqah and Stanslas, Johnson and Kirby, Brian Patrick and Neela, Vasantha Kumari and Ramasamy, Rajesh and Basri, Hamidon (2024) In Vitro blood-brain barrier models for neuroinfectious diseases: a narrative review. Current Neuropharmacology, 22 (8). pp. 1344-1373. ISSN 1570-159X; ESSN: 1875-6190 https://www.eurekaselect.com/article/136574 10.2174/1570159x22666231207114346 |
| spellingShingle | Badawi, Ahmad Hussein Mohamad, Nur Afiqah Stanslas, Johnson Kirby, Brian Patrick Neela, Vasantha Kumari Ramasamy, Rajesh Basri, Hamidon In Vitro blood-brain barrier models for neuroinfectious diseases: a narrative review |
| title | In Vitro blood-brain barrier models for neuroinfectious diseases: a narrative review |
| title_full | In Vitro blood-brain barrier models for neuroinfectious diseases: a narrative review |
| title_fullStr | In Vitro blood-brain barrier models for neuroinfectious diseases: a narrative review |
| title_full_unstemmed | In Vitro blood-brain barrier models for neuroinfectious diseases: a narrative review |
| title_short | In Vitro blood-brain barrier models for neuroinfectious diseases: a narrative review |
| title_sort | in vitro blood-brain barrier models for neuroinfectious diseases: a narrative review |
| url | http://psasir.upm.edu.my/id/eprint/110260/ http://psasir.upm.edu.my/id/eprint/110260/ http://psasir.upm.edu.my/id/eprint/110260/ |