Induction of systemic resistance in rice plants against Burkholderia glumae infection by bioformulation of Streptomyces spp. under greenhouse conditions
The ability of plant growth-promoting Streptomyces (PGPS) against bacterial panicle blight (BPB) disease caused by Burkholderia glumae has been previously examined under laboratory and greenhouse conditions. To further harness their potential, the development of bioformulations containing PGPS is ne...
| Main Authors: | , , , , , , , |
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| Format: | Article |
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Elsevier BV
2023
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| Online Access: | http://psasir.upm.edu.my/id/eprint/110256/ |
| _version_ | 1848865474984542208 |
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| author | Ngalimat, Mohamad Syazwan Mohd Hata, Erneeza Zulperi, Dzarifah Ismail, Siti Izera Ismail, Mohd Razi Mohd Zainudin, Nur Ain Izzati Saidi, Noor Baity Yusof, Mohd Termizi |
| author_facet | Ngalimat, Mohamad Syazwan Mohd Hata, Erneeza Zulperi, Dzarifah Ismail, Siti Izera Ismail, Mohd Razi Mohd Zainudin, Nur Ain Izzati Saidi, Noor Baity Yusof, Mohd Termizi |
| author_sort | Ngalimat, Mohamad Syazwan |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The ability of plant growth-promoting Streptomyces (PGPS) against bacterial panicle blight (BPB) disease caused by Burkholderia glumae has been previously examined under laboratory and greenhouse conditions. To further harness their potential, the development of bioformulations containing PGPS is needed. This study investigates the efficacy of Streptomyces bioformulations in suppressing the growth of B. glumae under greenhouse conditions. The powder-based bioformulations of Streptomyces were prepared and characterized based on potential phytotoxic effect and shelf life stability. The efficacy of Streptomyces bioformulations in suppressing BPB disease was assessed according to the expression of Gns1 (β-1,3-glucanase), OsPR1a (pathogenesis-related class 1), Oswrky45 (SA-responsive) and OsJAMYB (JA-responsive) using RT-qPCR. The talc-kaolin-Streptomyces bioformulations (BioSTKR8, BioSJAS2 and BioSTBS5 containing Streptomyces corchorusii TKR8, Streptomyces corchorusii JAS2 and Streptomyces misionensis TBS5, respectively) showed no phytotoxic effect on rice plants. Bioformulations were more stable when stored under refrigeration than at room temperature, with more than 50 spore viability at 180 days after storage. Treatment with BioSTKR8 (7 ) and BioSTBS5 (10 ) was able to reduce BPB disease severity while increasing rice yield by up to 301 (BioSTKR8) and 170 (BioSTBS5) relative to control (100 ). Transcript levels of Gns1, OsPR1a, Oswrky45 and OsJAMYB were up-regulated by the treatment of BioSTKR8 and BioSTBS5 at 7 DPI compared to the control. Results suggest that BioSTKR8 and BioSTBS5 are very promising bioformulations to be utilized as potential plant growth promoters and biological control agents (BCA) for sustainable rice production and BPB disease management. |
| first_indexed | 2025-11-15T14:05:17Z |
| format | Article |
| id | upm-110256 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T14:05:17Z |
| publishDate | 2023 |
| publisher | Elsevier BV |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1102562024-06-20T04:31:19Z http://psasir.upm.edu.my/id/eprint/110256/ Induction of systemic resistance in rice plants against Burkholderia glumae infection by bioformulation of Streptomyces spp. under greenhouse conditions Ngalimat, Mohamad Syazwan Mohd Hata, Erneeza Zulperi, Dzarifah Ismail, Siti Izera Ismail, Mohd Razi Mohd Zainudin, Nur Ain Izzati Saidi, Noor Baity Yusof, Mohd Termizi The ability of plant growth-promoting Streptomyces (PGPS) against bacterial panicle blight (BPB) disease caused by Burkholderia glumae has been previously examined under laboratory and greenhouse conditions. To further harness their potential, the development of bioformulations containing PGPS is needed. This study investigates the efficacy of Streptomyces bioformulations in suppressing the growth of B. glumae under greenhouse conditions. The powder-based bioformulations of Streptomyces were prepared and characterized based on potential phytotoxic effect and shelf life stability. The efficacy of Streptomyces bioformulations in suppressing BPB disease was assessed according to the expression of Gns1 (β-1,3-glucanase), OsPR1a (pathogenesis-related class 1), Oswrky45 (SA-responsive) and OsJAMYB (JA-responsive) using RT-qPCR. The talc-kaolin-Streptomyces bioformulations (BioSTKR8, BioSJAS2 and BioSTBS5 containing Streptomyces corchorusii TKR8, Streptomyces corchorusii JAS2 and Streptomyces misionensis TBS5, respectively) showed no phytotoxic effect on rice plants. Bioformulations were more stable when stored under refrigeration than at room temperature, with more than 50 spore viability at 180 days after storage. Treatment with BioSTKR8 (7 ) and BioSTBS5 (10 ) was able to reduce BPB disease severity while increasing rice yield by up to 301 (BioSTKR8) and 170 (BioSTBS5) relative to control (100 ). Transcript levels of Gns1, OsPR1a, Oswrky45 and OsJAMYB were up-regulated by the treatment of BioSTKR8 and BioSTBS5 at 7 DPI compared to the control. Results suggest that BioSTKR8 and BioSTBS5 are very promising bioformulations to be utilized as potential plant growth promoters and biological control agents (BCA) for sustainable rice production and BPB disease management. Elsevier BV 2023 Article PeerReviewed Ngalimat, Mohamad Syazwan and Mohd Hata, Erneeza and Zulperi, Dzarifah and Ismail, Siti Izera and Ismail, Mohd Razi and Mohd Zainudin, Nur Ain Izzati and Saidi, Noor Baity and Yusof, Mohd Termizi (2023) Induction of systemic resistance in rice plants against Burkholderia glumae infection by bioformulation of Streptomyces spp. under greenhouse conditions. Biological Control, 184. art. no. 105286. pp. 1-13. ISSN 1049-9644; ESSN: 1090-2112 https://linkinghub.elsevier.com/retrieve/pii/S1049964423001391 10.1016/j.biocontrol.2023.105286 |
| spellingShingle | Ngalimat, Mohamad Syazwan Mohd Hata, Erneeza Zulperi, Dzarifah Ismail, Siti Izera Ismail, Mohd Razi Mohd Zainudin, Nur Ain Izzati Saidi, Noor Baity Yusof, Mohd Termizi Induction of systemic resistance in rice plants against Burkholderia glumae infection by bioformulation of Streptomyces spp. under greenhouse conditions |
| title | Induction of systemic resistance in rice plants against Burkholderia glumae infection by bioformulation of Streptomyces spp. under greenhouse conditions |
| title_full | Induction of systemic resistance in rice plants against Burkholderia glumae infection by bioformulation of Streptomyces spp. under greenhouse conditions |
| title_fullStr | Induction of systemic resistance in rice plants against Burkholderia glumae infection by bioformulation of Streptomyces spp. under greenhouse conditions |
| title_full_unstemmed | Induction of systemic resistance in rice plants against Burkholderia glumae infection by bioformulation of Streptomyces spp. under greenhouse conditions |
| title_short | Induction of systemic resistance in rice plants against Burkholderia glumae infection by bioformulation of Streptomyces spp. under greenhouse conditions |
| title_sort | induction of systemic resistance in rice plants against burkholderia glumae infection by bioformulation of streptomyces spp. under greenhouse conditions |
| url | http://psasir.upm.edu.my/id/eprint/110256/ http://psasir.upm.edu.my/id/eprint/110256/ http://psasir.upm.edu.my/id/eprint/110256/ |