Recent advances in overexpression of functional recombinant lipases
Heterologous functional expression of the recombinant lipases is typically a bottleneck due to the expression in the insoluble fraction as inclusion bodies (IBs) which are in inactive form. Due to the importance of lipases in various industrial applications, many investigations have been conducted t...
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| Format: | Article |
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Springer Nature
2023
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| Online Access: | http://psasir.upm.edu.my/id/eprint/110093/ |
| _version_ | 1848865431888068608 |
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| author | Alias, Fatin Liyana Nezhad, Nima Ghahremani Normi, Yahaya M. Mohamad Ali, Mohd Shukuri Budiman, Cahyo Thean, Chor Leow |
| author_facet | Alias, Fatin Liyana Nezhad, Nima Ghahremani Normi, Yahaya M. Mohamad Ali, Mohd Shukuri Budiman, Cahyo Thean, Chor Leow |
| author_sort | Alias, Fatin Liyana |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Heterologous functional expression of the recombinant lipases is typically a bottleneck due to the expression in the insoluble fraction as inclusion bodies (IBs) which are in inactive form. Due to the importance of lipases in various industrial applications, many investigations have been conducted to discover suitable approaches to obtain functional lipase or increase the expressed yield in the soluble fraction. The utilization of the appropriate prokaryotic and eukaryotic expression systems, along with the suitable vectors, promoters, and tags, has been recognized as a practical approach. One of the most powerful strategies to produce bioactive lipases is using the molecular chaperones co-expressed along with the target protein’s genes into the expression host to produce the lipase in soluble fraction as a bioactive form. The refolding of expressed lipase from IBs (inactive) is another practical strategy which is usually carried out through chemical and physical methods. Based on recent investigations, the current review simultaneously highlights strategies to express the bioactive lipases and recover the bioactive lipases from the IBs in insoluble form. |
| first_indexed | 2025-11-15T14:04:36Z |
| format | Article |
| id | upm-110093 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T14:04:36Z |
| publishDate | 2023 |
| publisher | Springer Nature |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1100932024-09-05T06:51:45Z http://psasir.upm.edu.my/id/eprint/110093/ Recent advances in overexpression of functional recombinant lipases Alias, Fatin Liyana Nezhad, Nima Ghahremani Normi, Yahaya M. Mohamad Ali, Mohd Shukuri Budiman, Cahyo Thean, Chor Leow Heterologous functional expression of the recombinant lipases is typically a bottleneck due to the expression in the insoluble fraction as inclusion bodies (IBs) which are in inactive form. Due to the importance of lipases in various industrial applications, many investigations have been conducted to discover suitable approaches to obtain functional lipase or increase the expressed yield in the soluble fraction. The utilization of the appropriate prokaryotic and eukaryotic expression systems, along with the suitable vectors, promoters, and tags, has been recognized as a practical approach. One of the most powerful strategies to produce bioactive lipases is using the molecular chaperones co-expressed along with the target protein’s genes into the expression host to produce the lipase in soluble fraction as a bioactive form. The refolding of expressed lipase from IBs (inactive) is another practical strategy which is usually carried out through chemical and physical methods. Based on recent investigations, the current review simultaneously highlights strategies to express the bioactive lipases and recover the bioactive lipases from the IBs in insoluble form. Springer Nature 2023 Article PeerReviewed Alias, Fatin Liyana and Nezhad, Nima Ghahremani and Normi, Yahaya M. and Mohamad Ali, Mohd Shukuri and Budiman, Cahyo and Thean, Chor Leow (2023) Recent advances in overexpression of functional recombinant lipases. Molecular Biotechnology, 65. pp. 1737-1749. ISSN 1073-6085; ESSN: 1559-0305 https://link.springer.com/article/10.1007/s12033-023-00725-y?error=cookies_not_supported&code=9b03afcb-715c-4b1f-b962-b485a61a31da 10.1007/s12033-023-00725-y |
| spellingShingle | Alias, Fatin Liyana Nezhad, Nima Ghahremani Normi, Yahaya M. Mohamad Ali, Mohd Shukuri Budiman, Cahyo Thean, Chor Leow Recent advances in overexpression of functional recombinant lipases |
| title | Recent advances in overexpression of functional recombinant lipases |
| title_full | Recent advances in overexpression of functional recombinant lipases |
| title_fullStr | Recent advances in overexpression of functional recombinant lipases |
| title_full_unstemmed | Recent advances in overexpression of functional recombinant lipases |
| title_short | Recent advances in overexpression of functional recombinant lipases |
| title_sort | recent advances in overexpression of functional recombinant lipases |
| url | http://psasir.upm.edu.my/id/eprint/110093/ http://psasir.upm.edu.my/id/eprint/110093/ http://psasir.upm.edu.my/id/eprint/110093/ |