Nutrient deficiencies impact on the cellular and metabolic responses of saxitoxin producing Alexandrium minutum: A transcriptomic perspective
Dinoflagellate Alexandrium minutum Halim is commonly associated with harmful algal blooms (HABs) in tropical marine waters due to its saxitoxin production. However, limited information is available regarding the cellular and metabolic changes of A. minutum in nutrient-deficient environments. To fill...
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| Format: | Article |
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Human Resources Management Academic Research Society (HRMARS)
2023
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| Online Access: | http://psasir.upm.edu.my/id/eprint/110069/ |
| _version_ | 1848865424399138816 |
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| author | Akbar, Muhamad Afiq Mohd Yusof, Nurul Yuziana Usup, Gires Ahmad, Asmat Baharum, Syarul Nataqain Bunawan, Hamidun |
| author_facet | Akbar, Muhamad Afiq Mohd Yusof, Nurul Yuziana Usup, Gires Ahmad, Asmat Baharum, Syarul Nataqain Bunawan, Hamidun |
| author_sort | Akbar, Muhamad Afiq |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Dinoflagellate Alexandrium minutum Halim is commonly associated with harmful algal blooms (HABs) in tropical marine waters due to its saxitoxin production. However, limited information is available regarding the cellular and metabolic changes of A. minutum in nutrient-deficient environments. To fill this gap, our study aimed to investigate the transcriptomic responses of A. minutum under nitrogen and phosphorus deficiency. The induction of nitrogen and phosphorus deficiency resulted in the identification of 1049 and 763 differently expressed genes (DEGs), respectively. Further analysis using gene set enrichment analysis (GSEA) revealed 702 and 1251 enriched gene ontology (GO) terms associated with nitrogen and phosphorus deficiency, respectively. Our results indicate that in laboratory cultures, nitrogen deficiency primarily affects meiosis, carbohydrate catabolism, ammonium assimilation, ion homeostasis, and protein kinase activity. On the other hand, phosphorus deficiency primarily affects the carbon metabolic response, cellular ion transfer, actin-dependent cell movement, signalling pathways, and protein recycling. Our study provides valuable insights into biological processes and genes regulating A. minutum’s response to nutrient deficiencies, furthering our understanding of the ecophysiological response of HABs to environmental change. |
| first_indexed | 2025-11-15T14:04:29Z |
| format | Article |
| id | upm-110069 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T14:04:29Z |
| publishDate | 2023 |
| publisher | Human Resources Management Academic Research Society (HRMARS) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1100692024-09-05T07:26:15Z http://psasir.upm.edu.my/id/eprint/110069/ Nutrient deficiencies impact on the cellular and metabolic responses of saxitoxin producing Alexandrium minutum: A transcriptomic perspective Akbar, Muhamad Afiq Mohd Yusof, Nurul Yuziana Usup, Gires Ahmad, Asmat Baharum, Syarul Nataqain Bunawan, Hamidun Dinoflagellate Alexandrium minutum Halim is commonly associated with harmful algal blooms (HABs) in tropical marine waters due to its saxitoxin production. However, limited information is available regarding the cellular and metabolic changes of A. minutum in nutrient-deficient environments. To fill this gap, our study aimed to investigate the transcriptomic responses of A. minutum under nitrogen and phosphorus deficiency. The induction of nitrogen and phosphorus deficiency resulted in the identification of 1049 and 763 differently expressed genes (DEGs), respectively. Further analysis using gene set enrichment analysis (GSEA) revealed 702 and 1251 enriched gene ontology (GO) terms associated with nitrogen and phosphorus deficiency, respectively. Our results indicate that in laboratory cultures, nitrogen deficiency primarily affects meiosis, carbohydrate catabolism, ammonium assimilation, ion homeostasis, and protein kinase activity. On the other hand, phosphorus deficiency primarily affects the carbon metabolic response, cellular ion transfer, actin-dependent cell movement, signalling pathways, and protein recycling. Our study provides valuable insights into biological processes and genes regulating A. minutum’s response to nutrient deficiencies, furthering our understanding of the ecophysiological response of HABs to environmental change. Human Resources Management Academic Research Society (HRMARS) 2023 Article PeerReviewed Akbar, Muhamad Afiq and Mohd Yusof, Nurul Yuziana and Usup, Gires and Ahmad, Asmat and Baharum, Syarul Nataqain and Bunawan, Hamidun (2023) Nutrient deficiencies impact on the cellular and metabolic responses of saxitoxin producing Alexandrium minutum: A transcriptomic perspective. Marine Drugs, 21 (9). art. no. 497. pp. 1-18. ISSN 1660-3397 https://www.mdpi.com/1660-3397/21/9/497 10.3390/md21090497 |
| spellingShingle | Akbar, Muhamad Afiq Mohd Yusof, Nurul Yuziana Usup, Gires Ahmad, Asmat Baharum, Syarul Nataqain Bunawan, Hamidun Nutrient deficiencies impact on the cellular and metabolic responses of saxitoxin producing Alexandrium minutum: A transcriptomic perspective |
| title | Nutrient deficiencies impact on the cellular and metabolic responses of saxitoxin producing Alexandrium minutum: A transcriptomic perspective |
| title_full | Nutrient deficiencies impact on the cellular and metabolic responses of saxitoxin producing Alexandrium minutum: A transcriptomic perspective |
| title_fullStr | Nutrient deficiencies impact on the cellular and metabolic responses of saxitoxin producing Alexandrium minutum: A transcriptomic perspective |
| title_full_unstemmed | Nutrient deficiencies impact on the cellular and metabolic responses of saxitoxin producing Alexandrium minutum: A transcriptomic perspective |
| title_short | Nutrient deficiencies impact on the cellular and metabolic responses of saxitoxin producing Alexandrium minutum: A transcriptomic perspective |
| title_sort | nutrient deficiencies impact on the cellular and metabolic responses of saxitoxin producing alexandrium minutum: a transcriptomic perspective |
| url | http://psasir.upm.edu.my/id/eprint/110069/ http://psasir.upm.edu.my/id/eprint/110069/ http://psasir.upm.edu.my/id/eprint/110069/ |