Primer pairs specificity test for frog meat identification using PCR technique
Halal food assurance is becoming more important with the growth of the halal industry globally. Adulteration of halal meat products using non-halal sources such as pork, dog, boar, and even frog meat has become a major problem for moslems. The purpose of this study is to initiate the method for frog...
| Main Authors: | , , , , , , |
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| Format: | Article |
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Universitas Gadjah Mada, Indonesia
2023
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| Online Access: | http://psasir.upm.edu.my/id/eprint/110064/ |
| _version_ | 1848865422915403776 |
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| author | Haryono, Norman Yoshi Khusufi, Rizqi Layli Pangesti, Delia Wahyu Susanti, Evi Mariana, Rina Rifqie Wardani, Hartati Eko Salim, Norazlinaliza |
| author_facet | Haryono, Norman Yoshi Khusufi, Rizqi Layli Pangesti, Delia Wahyu Susanti, Evi Mariana, Rina Rifqie Wardani, Hartati Eko Salim, Norazlinaliza |
| author_sort | Haryono, Norman Yoshi |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Halal food assurance is becoming more important with the growth of the halal industry globally. Adulteration of halal meat products using non-halal sources such as pork, dog, boar, and even frog meat has become a major problem for moslems. The purpose of this study is to initiate the method for frog meat identification using polymerase chain reaction (PCR) technique. In this study, three primer pairs (Fk1-Rk1, Fk2-Rk2, Fk3-Rk3) were analyzed for their specificity toward frog meat against other common halal meat sources such as beef, chicken, shrimp, squid, and mackerel. The visualization of DNA amplification showed that primer pair Fk1-Rk1 produced primer-dimer, thus cannot be used for this circumstance. Primer pair Fk2-Rk2 showed a better result where DNA amplicon was produced at ~100 bp for frog meat and no amplicons for other meat. Primer pair Fk3-Rk3 showed a different pattern of DNA amplification for all the meat tested, where the amplicon of frog meat was shown at ~100 bp, while the other meat showed multiple amplicons or none. In conclusion, primer pairs Fk2-Rk2 and Fk3-Rk3 showed their potential as primer pairs for frog meat identification using PCR for implementing halal food assurance, although sensitivity analysis needs to be investigated. |
| first_indexed | 2025-11-15T14:04:28Z |
| format | Article |
| id | upm-110064 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T14:04:28Z |
| publishDate | 2023 |
| publisher | Universitas Gadjah Mada, Indonesia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1100642024-09-05T07:26:36Z http://psasir.upm.edu.my/id/eprint/110064/ Primer pairs specificity test for frog meat identification using PCR technique Haryono, Norman Yoshi Khusufi, Rizqi Layli Pangesti, Delia Wahyu Susanti, Evi Mariana, Rina Rifqie Wardani, Hartati Eko Salim, Norazlinaliza Halal food assurance is becoming more important with the growth of the halal industry globally. Adulteration of halal meat products using non-halal sources such as pork, dog, boar, and even frog meat has become a major problem for moslems. The purpose of this study is to initiate the method for frog meat identification using polymerase chain reaction (PCR) technique. In this study, three primer pairs (Fk1-Rk1, Fk2-Rk2, Fk3-Rk3) were analyzed for their specificity toward frog meat against other common halal meat sources such as beef, chicken, shrimp, squid, and mackerel. The visualization of DNA amplification showed that primer pair Fk1-Rk1 produced primer-dimer, thus cannot be used for this circumstance. Primer pair Fk2-Rk2 showed a better result where DNA amplicon was produced at ~100 bp for frog meat and no amplicons for other meat. Primer pair Fk3-Rk3 showed a different pattern of DNA amplification for all the meat tested, where the amplicon of frog meat was shown at ~100 bp, while the other meat showed multiple amplicons or none. In conclusion, primer pairs Fk2-Rk2 and Fk3-Rk3 showed their potential as primer pairs for frog meat identification using PCR for implementing halal food assurance, although sensitivity analysis needs to be investigated. Universitas Gadjah Mada, Indonesia 2023 Article PeerReviewed Haryono, Norman Yoshi and Khusufi, Rizqi Layli and Pangesti, Delia Wahyu and Susanti, Evi and Mariana, Rina Rifqie and Wardani, Hartati Eko and Salim, Norazlinaliza (2023) Primer pairs specificity test for frog meat identification using PCR technique. Indonesian Journal Of Chemistry, 23 (5). 1315 - 1323. ISSN 1411-9420; ESSN: 2460-1578 https://jurnal.ugm.ac.id/ijc/article/view/83626 10.22146/ijc.83626 |
| spellingShingle | Haryono, Norman Yoshi Khusufi, Rizqi Layli Pangesti, Delia Wahyu Susanti, Evi Mariana, Rina Rifqie Wardani, Hartati Eko Salim, Norazlinaliza Primer pairs specificity test for frog meat identification using PCR technique |
| title | Primer pairs specificity test for frog meat identification using PCR technique |
| title_full | Primer pairs specificity test for frog meat identification using PCR technique |
| title_fullStr | Primer pairs specificity test for frog meat identification using PCR technique |
| title_full_unstemmed | Primer pairs specificity test for frog meat identification using PCR technique |
| title_short | Primer pairs specificity test for frog meat identification using PCR technique |
| title_sort | primer pairs specificity test for frog meat identification using pcr technique |
| url | http://psasir.upm.edu.my/id/eprint/110064/ http://psasir.upm.edu.my/id/eprint/110064/ http://psasir.upm.edu.my/id/eprint/110064/ |