Low genetic diversity of vector-borne haemoparasites in dogs and their ticks revealed local and long-range transmission in Peninsular Malaysia
Molecular methods coupled with phylogenetic analysis are sensitive tools for detecting and classifying parasites. This study used nuclear and mitochondrial gene markers to investigate the host-vector interaction of the vector-borne haemoparasites. The population genetic structures of important vecto...
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| Format: | Article |
| Language: | English |
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Universiti Putra Malaysia
2023
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| Online Access: | http://psasir.upm.edu.my/id/eprint/109560/ http://psasir.upm.edu.my/id/eprint/109560/1/109560.pdf |
| _version_ | 1848865404494020608 |
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| author | Sipin, Quincie Mustaffa-Kamal, Farina Watanabe, Malaika Megat Abdul Rani, Puteri Azaziah Abdul Aziz, Nor Azlina |
| author_facet | Sipin, Quincie Mustaffa-Kamal, Farina Watanabe, Malaika Megat Abdul Rani, Puteri Azaziah Abdul Aziz, Nor Azlina |
| author_sort | Sipin, Quincie |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Molecular methods coupled with phylogenetic analysis are sensitive tools for detecting and classifying parasites. This study used nuclear and mitochondrial gene markers to investigate the host-vector interaction of the vector-borne haemoparasites. The population genetic structures of important vector-borne haemoparasites in dogs, namely, Anaplasma platys, Ehrlichia canis, Babesia vogeli, and Babesia gibsoni, were determined from the nuclear gene of 16S or 18S rRNA gene, gltA and groESL and mitochondrial gene of COX1 across dogs and vector ticks. A total of 220 blood samples and 140 ticks were collected from shelter dogs in Peninsular Malaysia. Out of the positive samples for the vector-borne haemoparasites, 28 positive blood isolates and six tick isolates were selected and characterised. There was a low diversity in tick sequences, while varying degree of variability was observed in dogs’ sequences. Overlapped haplotypes were observed in sequences of dogs and ticks, revealing the possibility of the same infection origin. No regional separation was detected, but similar haplotypes from different regions were observed. These findings contribute to the epidemiology of vector-borne haemoparasites in dogs in Malaysia. |
| first_indexed | 2025-11-15T14:04:10Z |
| format | Article |
| id | upm-109560 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:04:10Z |
| publishDate | 2023 |
| publisher | Universiti Putra Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1095602025-01-14T02:10:58Z http://psasir.upm.edu.my/id/eprint/109560/ Low genetic diversity of vector-borne haemoparasites in dogs and their ticks revealed local and long-range transmission in Peninsular Malaysia Sipin, Quincie Mustaffa-Kamal, Farina Watanabe, Malaika Megat Abdul Rani, Puteri Azaziah Abdul Aziz, Nor Azlina Molecular methods coupled with phylogenetic analysis are sensitive tools for detecting and classifying parasites. This study used nuclear and mitochondrial gene markers to investigate the host-vector interaction of the vector-borne haemoparasites. The population genetic structures of important vector-borne haemoparasites in dogs, namely, Anaplasma platys, Ehrlichia canis, Babesia vogeli, and Babesia gibsoni, were determined from the nuclear gene of 16S or 18S rRNA gene, gltA and groESL and mitochondrial gene of COX1 across dogs and vector ticks. A total of 220 blood samples and 140 ticks were collected from shelter dogs in Peninsular Malaysia. Out of the positive samples for the vector-borne haemoparasites, 28 positive blood isolates and six tick isolates were selected and characterised. There was a low diversity in tick sequences, while varying degree of variability was observed in dogs’ sequences. Overlapped haplotypes were observed in sequences of dogs and ticks, revealing the possibility of the same infection origin. No regional separation was detected, but similar haplotypes from different regions were observed. These findings contribute to the epidemiology of vector-borne haemoparasites in dogs in Malaysia. Universiti Putra Malaysia 2023-05-16 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/109560/1/109560.pdf Sipin, Quincie and Mustaffa-Kamal, Farina and Watanabe, Malaika and Megat Abdul Rani, Puteri Azaziah and Abdul Aziz, Nor Azlina (2023) Low genetic diversity of vector-borne haemoparasites in dogs and their ticks revealed local and long-range transmission in Peninsular Malaysia. Pertanika Journal of Tropical Agricultural Science, 46 (2). pp. 401-423. ISSN 1511-3701; eISSN: 2231-8542 http://www.pertanika.upm.edu.my/pjtas/browse/regular-issue?article=JTAS-2554-2022 10.47836/pjtas.46.2.03 |
| spellingShingle | Sipin, Quincie Mustaffa-Kamal, Farina Watanabe, Malaika Megat Abdul Rani, Puteri Azaziah Abdul Aziz, Nor Azlina Low genetic diversity of vector-borne haemoparasites in dogs and their ticks revealed local and long-range transmission in Peninsular Malaysia |
| title | Low genetic diversity of vector-borne haemoparasites in dogs and their ticks revealed local and long-range transmission in Peninsular Malaysia |
| title_full | Low genetic diversity of vector-borne haemoparasites in dogs and their ticks revealed local and long-range transmission in Peninsular Malaysia |
| title_fullStr | Low genetic diversity of vector-borne haemoparasites in dogs and their ticks revealed local and long-range transmission in Peninsular Malaysia |
| title_full_unstemmed | Low genetic diversity of vector-borne haemoparasites in dogs and their ticks revealed local and long-range transmission in Peninsular Malaysia |
| title_short | Low genetic diversity of vector-borne haemoparasites in dogs and their ticks revealed local and long-range transmission in Peninsular Malaysia |
| title_sort | low genetic diversity of vector-borne haemoparasites in dogs and their ticks revealed local and long-range transmission in peninsular malaysia |
| url | http://psasir.upm.edu.my/id/eprint/109560/ http://psasir.upm.edu.my/id/eprint/109560/ http://psasir.upm.edu.my/id/eprint/109560/ http://psasir.upm.edu.my/id/eprint/109560/1/109560.pdf |