In vivo toxicity assessment of the probiotic Bacillus amyloliquefaciens HTI-19 isolated from stingless bee (Heterotrigona itama) honey
This study evaluated the acute and sub-acute toxicity of B. amyloliquefaciens HTI-19 (isolated from stingless bee honey) in female Sprague Dawley rats. In an acute toxicity study, the rats received a low dosage (1 × 109 CFU·mL−1 ), medium dosage (3 × 109 CFU·mL−1 ), or high dosage (1 × 1010 CFU·mL−1...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Published: |
Multidisciplinary Digital Publishing Institute
2023
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/109403/ |
| _version_ | 1848865361748819968 |
|---|---|
| author | Zulkhairi Amin, Fatin Aina Shafiq Cheng, Mohamad Zulhafiz Sabri, Suriana Ismail, Norsharina Chan, Kim Wei Mohd Esa, Norhaizan Mohd Lila, Mohd Azmi Nur-Fazila, Saulol Hamid Khalifa, Shaden A. M. El-Seedi, Hesham R. Zawawi, Norhasnida |
| author_facet | Zulkhairi Amin, Fatin Aina Shafiq Cheng, Mohamad Zulhafiz Sabri, Suriana Ismail, Norsharina Chan, Kim Wei Mohd Esa, Norhaizan Mohd Lila, Mohd Azmi Nur-Fazila, Saulol Hamid Khalifa, Shaden A. M. El-Seedi, Hesham R. Zawawi, Norhasnida |
| author_sort | Zulkhairi Amin, Fatin Aina |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | This study evaluated the acute and sub-acute toxicity of B. amyloliquefaciens HTI-19 (isolated from stingless bee honey) in female Sprague Dawley rats. In an acute toxicity study, the rats received a low dosage (1 × 109 CFU·mL−1 ), medium dosage (3 × 109 CFU·mL−1 ), or high dosage (1 × 1010 CFU·mL−1 ) of B. amyloliquefaciens HTI-19 daily orally by syringe-feeding for 14 days. For the subacute toxicity study, rats received a low dosage (1 × 109 CFU·mL−1 ) or a high dosage (1 × 1010 CFU·mL−1 ) for 28 days. The probiotic feeding in acute and sub-acute toxicity studies showed no mortality or significant abnormalities in rats throughout the experimental period. In week 2 of the acute study, the body weight of the rats showed a significant increase (p < 0.05) compared to the control. By gross and microscopic examination of organs, no evidently significant changes were observed in the morphology of organs. Serum biochemical tests and blood hematology tests also revealed no treatment-related changes. Overall, these data indicated that oral administration of B. amyloliquefaciens HTI-19 up to 1 × 109 CFU·mL−1 for 28 days can be considered safe. |
| first_indexed | 2025-11-15T14:03:29Z |
| format | Article |
| id | upm-109403 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T14:03:29Z |
| publishDate | 2023 |
| publisher | Multidisciplinary Digital Publishing Institute |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1094032024-08-05T02:59:10Z http://psasir.upm.edu.my/id/eprint/109403/ In vivo toxicity assessment of the probiotic Bacillus amyloliquefaciens HTI-19 isolated from stingless bee (Heterotrigona itama) honey Zulkhairi Amin, Fatin Aina Shafiq Cheng, Mohamad Zulhafiz Sabri, Suriana Ismail, Norsharina Chan, Kim Wei Mohd Esa, Norhaizan Mohd Lila, Mohd Azmi Nur-Fazila, Saulol Hamid Khalifa, Shaden A. M. El-Seedi, Hesham R. Zawawi, Norhasnida This study evaluated the acute and sub-acute toxicity of B. amyloliquefaciens HTI-19 (isolated from stingless bee honey) in female Sprague Dawley rats. In an acute toxicity study, the rats received a low dosage (1 × 109 CFU·mL−1 ), medium dosage (3 × 109 CFU·mL−1 ), or high dosage (1 × 1010 CFU·mL−1 ) of B. amyloliquefaciens HTI-19 daily orally by syringe-feeding for 14 days. For the subacute toxicity study, rats received a low dosage (1 × 109 CFU·mL−1 ) or a high dosage (1 × 1010 CFU·mL−1 ) for 28 days. The probiotic feeding in acute and sub-acute toxicity studies showed no mortality or significant abnormalities in rats throughout the experimental period. In week 2 of the acute study, the body weight of the rats showed a significant increase (p < 0.05) compared to the control. By gross and microscopic examination of organs, no evidently significant changes were observed in the morphology of organs. Serum biochemical tests and blood hematology tests also revealed no treatment-related changes. Overall, these data indicated that oral administration of B. amyloliquefaciens HTI-19 up to 1 × 109 CFU·mL−1 for 28 days can be considered safe. Multidisciplinary Digital Publishing Institute 2023-04-05 Article PeerReviewed Zulkhairi Amin, Fatin Aina and Shafiq Cheng, Mohamad Zulhafiz and Sabri, Suriana and Ismail, Norsharina and Chan, Kim Wei and Mohd Esa, Norhaizan and Mohd Lila, Mohd Azmi and Nur-Fazila, Saulol Hamid and Khalifa, Shaden A. M. and El-Seedi, Hesham R. and Zawawi, Norhasnida (2023) In vivo toxicity assessment of the probiotic Bacillus amyloliquefaciens HTI-19 isolated from stingless bee (Heterotrigona itama) honey. Nutrients, 15 (10). art. no. 2390. pp. 1-16. ISSN 2072-6643 https://www.mdpi.com/2072-6643/15/10/2390#:~:text=This%20study%20assessed%20the%20toxicity,administered%20to%20the%20treated%20rats. 10.3390/nu15102390 |
| spellingShingle | Zulkhairi Amin, Fatin Aina Shafiq Cheng, Mohamad Zulhafiz Sabri, Suriana Ismail, Norsharina Chan, Kim Wei Mohd Esa, Norhaizan Mohd Lila, Mohd Azmi Nur-Fazila, Saulol Hamid Khalifa, Shaden A. M. El-Seedi, Hesham R. Zawawi, Norhasnida In vivo toxicity assessment of the probiotic Bacillus amyloliquefaciens HTI-19 isolated from stingless bee (Heterotrigona itama) honey |
| title | In vivo toxicity assessment of the probiotic Bacillus amyloliquefaciens HTI-19 isolated from stingless bee (Heterotrigona itama) honey |
| title_full | In vivo toxicity assessment of the probiotic Bacillus amyloliquefaciens HTI-19 isolated from stingless bee (Heterotrigona itama) honey |
| title_fullStr | In vivo toxicity assessment of the probiotic Bacillus amyloliquefaciens HTI-19 isolated from stingless bee (Heterotrigona itama) honey |
| title_full_unstemmed | In vivo toxicity assessment of the probiotic Bacillus amyloliquefaciens HTI-19 isolated from stingless bee (Heterotrigona itama) honey |
| title_short | In vivo toxicity assessment of the probiotic Bacillus amyloliquefaciens HTI-19 isolated from stingless bee (Heterotrigona itama) honey |
| title_sort | in vivo toxicity assessment of the probiotic bacillus amyloliquefaciens hti-19 isolated from stingless bee (heterotrigona itama) honey |
| url | http://psasir.upm.edu.my/id/eprint/109403/ http://psasir.upm.edu.my/id/eprint/109403/ http://psasir.upm.edu.my/id/eprint/109403/ |