ITS-PCR-RFLP analysis of Ganoderma sp. infecting industrial crops
Ganoderma is a disastrous pathogen that has been causing tremendous losses to economically important crops in many countries. Vast genetic variations have been observed among several Ganoderma species, even from the same host. In this study, genetic variation was assessed among 44 isolates of Ganode...
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Universiti Putra Malaysia Press
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/10911/ http://psasir.upm.edu.my/id/eprint/10911/1/ITS-PCR-RFLP%20Analysis%20of%20Ganoderma%20sp.%20Infecting%20Industrial%20Crops.pdf |
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| author | Syd Ali, Nusaibah Zakaria, Latiffah A. R., Hassaan |
| author_facet | Syd Ali, Nusaibah Zakaria, Latiffah A. R., Hassaan |
| author_sort | Syd Ali, Nusaibah |
| building | UPM Institutional Repository |
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| description | Ganoderma is a disastrous pathogen that has been causing tremendous losses to economically important crops in many countries. Vast genetic variations have been observed among several Ganoderma species, even from the same host. In this study, genetic variation was assessed among 44 isolates of Ganoderma sp. isolated from the basidiocarps of four different hosts (oil palm, rubber, tea, and forest trees) collected from selected areas of Peninsular Malaysia. Restriction Fragment Length Polymorphism (RFLP) technique, using ITS1 and ITS4 primers, was used to amplify Internal Transcribed Spacer (ITS) regions. Amplified products were further digested using Bsu 151, Hind III and Taq I restriction enzymes. Cluster analysis with UPGMA using genetic distances clustered all the isolates studied into four main groups. Generally, Ganoderma isolates from the same host were clustered together. The isolates from tea and rubber were more closely related compared to oil palm and forest trees. Similarly, the Ganoderma isolates from the same host were also clustered together, and three species were identified, namely, G. boninense (from oil palm and coconut stumps), G. philippii (rubber) and G. australe (forest trees). The results obtained from the analysis showed that host preference was a possible factor in the differentiation of Ganoderma species. |
| first_indexed | 2025-11-15T07:44:36Z |
| format | Article |
| id | upm-10911 |
| institution | Universiti Putra Malaysia |
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| language | English |
| last_indexed | 2025-11-15T07:44:36Z |
| publishDate | 2011 |
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| spelling | upm-109112015-11-03T02:01:00Z http://psasir.upm.edu.my/id/eprint/10911/ ITS-PCR-RFLP analysis of Ganoderma sp. infecting industrial crops Syd Ali, Nusaibah Zakaria, Latiffah A. R., Hassaan Ganoderma is a disastrous pathogen that has been causing tremendous losses to economically important crops in many countries. Vast genetic variations have been observed among several Ganoderma species, even from the same host. In this study, genetic variation was assessed among 44 isolates of Ganoderma sp. isolated from the basidiocarps of four different hosts (oil palm, rubber, tea, and forest trees) collected from selected areas of Peninsular Malaysia. Restriction Fragment Length Polymorphism (RFLP) technique, using ITS1 and ITS4 primers, was used to amplify Internal Transcribed Spacer (ITS) regions. Amplified products were further digested using Bsu 151, Hind III and Taq I restriction enzymes. Cluster analysis with UPGMA using genetic distances clustered all the isolates studied into four main groups. Generally, Ganoderma isolates from the same host were clustered together. The isolates from tea and rubber were more closely related compared to oil palm and forest trees. Similarly, the Ganoderma isolates from the same host were also clustered together, and three species were identified, namely, G. boninense (from oil palm and coconut stumps), G. philippii (rubber) and G. australe (forest trees). The results obtained from the analysis showed that host preference was a possible factor in the differentiation of Ganoderma species. Universiti Putra Malaysia Press 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/10911/1/ITS-PCR-RFLP%20Analysis%20of%20Ganoderma%20sp.%20Infecting%20Industrial%20Crops.pdf Syd Ali, Nusaibah and Zakaria, Latiffah and A. R., Hassaan (2011) ITS-PCR-RFLP analysis of Ganoderma sp. infecting industrial crops. Pertanika Journal of Tropical Agricultural Science, 34 (1). pp. 83-91. ISSN 1511-3701; ESSN: 2231-8542 http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2034%20(1)%20Feb.%202011/12%20Pg%2083-92.pdf |
| spellingShingle | Syd Ali, Nusaibah Zakaria, Latiffah A. R., Hassaan ITS-PCR-RFLP analysis of Ganoderma sp. infecting industrial crops |
| title | ITS-PCR-RFLP analysis of Ganoderma sp. infecting industrial crops |
| title_full | ITS-PCR-RFLP analysis of Ganoderma sp. infecting industrial crops |
| title_fullStr | ITS-PCR-RFLP analysis of Ganoderma sp. infecting industrial crops |
| title_full_unstemmed | ITS-PCR-RFLP analysis of Ganoderma sp. infecting industrial crops |
| title_short | ITS-PCR-RFLP analysis of Ganoderma sp. infecting industrial crops |
| title_sort | its-pcr-rflp analysis of ganoderma sp. infecting industrial crops |
| url | http://psasir.upm.edu.my/id/eprint/10911/ http://psasir.upm.edu.my/id/eprint/10911/ http://psasir.upm.edu.my/id/eprint/10911/1/ITS-PCR-RFLP%20Analysis%20of%20Ganoderma%20sp.%20Infecting%20Industrial%20Crops.pdf |