Screening and selection of formulation components of nanostructured lipid carriers system for Mitragyna speciosa (Korth). Havil drug delivery
Nanostructured lipid carriers (NLC) have evolved as effective vehicles for improving the bioavailability of poorly water-soluble drugs as well as protecting them from degradation and metabolism. The present study investigated the preformulation and its impact on different liquid lipids and solid l...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Published: |
Elsevier
2023
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| Online Access: | http://psasir.upm.edu.my/id/eprint/109087/ |
| _version_ | 1848865280284950528 |
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| author | Syed Azhar, Sharifah Nurfadhlin Afifah Ashari, Siti Efliza Tan, Jen Kit Kassim, Nur Kartinee Hassan, Masriana Zainuddin, Norhazlin Mohamad, Rosfarizan Mat Azmi, Intan Diana |
| author_facet | Syed Azhar, Sharifah Nurfadhlin Afifah Ashari, Siti Efliza Tan, Jen Kit Kassim, Nur Kartinee Hassan, Masriana Zainuddin, Norhazlin Mohamad, Rosfarizan Mat Azmi, Intan Diana |
| author_sort | Syed Azhar, Sharifah Nurfadhlin Afifah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Nanostructured lipid carriers (NLC) have evolved as effective vehicles for improving the bioavailability of poorly
water-soluble drugs as well as protecting them from degradation and metabolism. The present study investigated
the preformulation and its impact on different liquid lipids and solid lipids with diverse chemical structures and
hydrophilic-lipophilic balance (HLB) for the critical quality attributes of Mitragyna speciosa leaves extract (MS)-
based nanostructured lipid carriers suitable for drug delivery system. The presence of bioactive alkaloids
(mitragynine, 7-hydroxymitragynine, speciogynine), flavonoids (quercetin, apigenin, kaempferol), saponins
(daucosterol, quinovic acid 3-o-beta-D-quinovopyranoside, 1-o-feruloyl-beta-D-glucose), and other bioactive
phytochemicals (chlorogenic acid, umbelliferone, ursolic acid) in MS leaves extract was detected using LCMS/MS
analysis to support its medicinal properties. The total phenolic content, total flavonoid content, and DPPH assay
of MS were 90.88 ± 0.30 GAE mg/g, 50.43 ± 0.47 QE mg/g, and IC50 at 0.0397 ± 0.0035 mg/mL, respectively.
The screening preparation of NLC requires the selection of liquid lipid (oil), solid lipid, and surfactant. Based on
the solubility test, the maximum solubility of MS leaves extract in oleic acid (liquid lipid) and compritol ATO 888
(solid lipid) were 2.859 ± 0.010% and 53.02 ± 0.39% respectively at ratio 70:30. Tween 80 was selected as the
main surfactant for the preparation of NLC due to its highest emulsification efficiency (51.53%). The antimicrobial study showed that MS leaves extract gives antimicrobial effect towards Streptococcus aureus bacteria. The
preformulation information collected offers a better, efficient, and cost-effective choice for further formulation
development of MS leaves extract in drug delivery systems. |
| first_indexed | 2025-11-15T14:02:12Z |
| format | Article |
| id | upm-109087 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T14:02:12Z |
| publishDate | 2023 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1090872024-10-15T02:22:34Z http://psasir.upm.edu.my/id/eprint/109087/ Screening and selection of formulation components of nanostructured lipid carriers system for Mitragyna speciosa (Korth). Havil drug delivery Syed Azhar, Sharifah Nurfadhlin Afifah Ashari, Siti Efliza Tan, Jen Kit Kassim, Nur Kartinee Hassan, Masriana Zainuddin, Norhazlin Mohamad, Rosfarizan Mat Azmi, Intan Diana Nanostructured lipid carriers (NLC) have evolved as effective vehicles for improving the bioavailability of poorly water-soluble drugs as well as protecting them from degradation and metabolism. The present study investigated the preformulation and its impact on different liquid lipids and solid lipids with diverse chemical structures and hydrophilic-lipophilic balance (HLB) for the critical quality attributes of Mitragyna speciosa leaves extract (MS)- based nanostructured lipid carriers suitable for drug delivery system. The presence of bioactive alkaloids (mitragynine, 7-hydroxymitragynine, speciogynine), flavonoids (quercetin, apigenin, kaempferol), saponins (daucosterol, quinovic acid 3-o-beta-D-quinovopyranoside, 1-o-feruloyl-beta-D-glucose), and other bioactive phytochemicals (chlorogenic acid, umbelliferone, ursolic acid) in MS leaves extract was detected using LCMS/MS analysis to support its medicinal properties. The total phenolic content, total flavonoid content, and DPPH assay of MS were 90.88 ± 0.30 GAE mg/g, 50.43 ± 0.47 QE mg/g, and IC50 at 0.0397 ± 0.0035 mg/mL, respectively. The screening preparation of NLC requires the selection of liquid lipid (oil), solid lipid, and surfactant. Based on the solubility test, the maximum solubility of MS leaves extract in oleic acid (liquid lipid) and compritol ATO 888 (solid lipid) were 2.859 ± 0.010% and 53.02 ± 0.39% respectively at ratio 70:30. Tween 80 was selected as the main surfactant for the preparation of NLC due to its highest emulsification efficiency (51.53%). The antimicrobial study showed that MS leaves extract gives antimicrobial effect towards Streptococcus aureus bacteria. The preformulation information collected offers a better, efficient, and cost-effective choice for further formulation development of MS leaves extract in drug delivery systems. Elsevier 2023-08 Article PeerReviewed Syed Azhar, Sharifah Nurfadhlin Afifah and Ashari, Siti Efliza and Tan, Jen Kit and Kassim, Nur Kartinee and Hassan, Masriana and Zainuddin, Norhazlin and Mohamad, Rosfarizan and Mat Azmi, Intan Diana (2023) Screening and selection of formulation components of nanostructured lipid carriers system for Mitragyna speciosa (Korth). Havil drug delivery. Industrial Crops and Products, 198. art. no. 116668. pp. 1-13. ISSN 0926-6690; ESSN: 1872-633X https://linkinghub.elsevier.com/retrieve/pii/S0926669023004338 10.1016/j.indcrop.2023.116668 |
| spellingShingle | Syed Azhar, Sharifah Nurfadhlin Afifah Ashari, Siti Efliza Tan, Jen Kit Kassim, Nur Kartinee Hassan, Masriana Zainuddin, Norhazlin Mohamad, Rosfarizan Mat Azmi, Intan Diana Screening and selection of formulation components of nanostructured lipid carriers system for Mitragyna speciosa (Korth). Havil drug delivery |
| title | Screening and selection of formulation components of nanostructured lipid carriers system for Mitragyna speciosa (Korth). Havil drug delivery |
| title_full | Screening and selection of formulation components of nanostructured lipid carriers system for Mitragyna speciosa (Korth). Havil drug delivery |
| title_fullStr | Screening and selection of formulation components of nanostructured lipid carriers system for Mitragyna speciosa (Korth). Havil drug delivery |
| title_full_unstemmed | Screening and selection of formulation components of nanostructured lipid carriers system for Mitragyna speciosa (Korth). Havil drug delivery |
| title_short | Screening and selection of formulation components of nanostructured lipid carriers system for Mitragyna speciosa (Korth). Havil drug delivery |
| title_sort | screening and selection of formulation components of nanostructured lipid carriers system for mitragyna speciosa (korth). havil drug delivery |
| url | http://psasir.upm.edu.my/id/eprint/109087/ http://psasir.upm.edu.my/id/eprint/109087/ http://psasir.upm.edu.my/id/eprint/109087/ |