The potential chemopreventive effect of Andrographis paniculata on 1,2-dimethylhydrazine and high-fat-diet-induced colorectal cancer in Sprague Dawley rats
Colorectal cancer (CRC) is responsible for a notable rise in the overall mortality rate. Obesity is found to be one of the main factors behind CRC development. Andrographis paniculata is a herbaceous plant famous for its medicinal properties, particularly in Southeast Asia for its anti-cancer proper...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
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MDPI
2023
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| Online Access: | http://psasir.upm.edu.my/id/eprint/109056/ |
| _version_ | 1848865271681384448 |
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| author | Subarmaniam, Tharani Mahmad Rusli, Rusydatul Nabila Perumal, Kokila Vani Yong, Yoke Keong Siti Hadizah Othman, Fezah Salem, Khaled Shafie, Nurul Husna Hasham, Rosnani Yin, Khoo Boon Abdul Kadir, Khairul Kamilah Bahari, Hasnah Zakaria, Zainul Amiruddin |
| author_facet | Subarmaniam, Tharani Mahmad Rusli, Rusydatul Nabila Perumal, Kokila Vani Yong, Yoke Keong Siti Hadizah Othman, Fezah Salem, Khaled Shafie, Nurul Husna Hasham, Rosnani Yin, Khoo Boon Abdul Kadir, Khairul Kamilah Bahari, Hasnah Zakaria, Zainul Amiruddin |
| author_sort | Subarmaniam, Tharani |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Colorectal cancer (CRC) is responsible for a notable rise in the overall mortality rate. Obesity is found to be one of the main factors behind CRC development. Andrographis paniculata is a herbaceous plant famous for its medicinal properties, particularly in Southeast Asia for its anti-cancer properties. This study examines the chemopreventive impact of A. paniculata ethanolic extract (APEE) against a high-fat diet and 1,2-dimethylhydrazine-induced colon cancer in Sprague Dawley rats. Sprague Dawley rats were administered 1,2-dimethylhydrazine (40 mg/kg, i.p. once a week for 10 weeks) and a high-fat diet (HFD) for 20 weeks to induce colorectal cancer. APEE was administered at 125 mg/kg, 250 mg/kg, and 500 mg/kg for 20 weeks. At the end of the experiment, blood serum and organs were collected. DMH/HFD-induced rats had abnormal crypts and more aberrant crypt foci (ACF). APEE at a dose of 500 mg/kg improved the dysplastic state of the colon tissue and caused a 32% reduction in the total ACF. HFD increased adipocyte cell size, while 500 mg/kg APEE reduced it. HFD and DMH/HFD rats had elevated serum insulin and leptin levels. Moreover, UHPLC-QTOF-MS analysis revealed that APEE was rich in anti-cancer phytochemicals. This finding suggests that APEE has anti-cancer potential against HFD/DMH-induced CRC and anti-adipogenic and anti-obesity properties. |
| first_indexed | 2025-11-15T14:02:04Z |
| format | Article |
| id | upm-109056 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T14:02:04Z |
| publishDate | 2023 |
| publisher | MDPI |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1090562024-10-15T04:25:11Z http://psasir.upm.edu.my/id/eprint/109056/ The potential chemopreventive effect of Andrographis paniculata on 1,2-dimethylhydrazine and high-fat-diet-induced colorectal cancer in Sprague Dawley rats Subarmaniam, Tharani Mahmad Rusli, Rusydatul Nabila Perumal, Kokila Vani Yong, Yoke Keong Siti Hadizah Othman, Fezah Salem, Khaled Shafie, Nurul Husna Hasham, Rosnani Yin, Khoo Boon Abdul Kadir, Khairul Kamilah Bahari, Hasnah Zakaria, Zainul Amiruddin Colorectal cancer (CRC) is responsible for a notable rise in the overall mortality rate. Obesity is found to be one of the main factors behind CRC development. Andrographis paniculata is a herbaceous plant famous for its medicinal properties, particularly in Southeast Asia for its anti-cancer properties. This study examines the chemopreventive impact of A. paniculata ethanolic extract (APEE) against a high-fat diet and 1,2-dimethylhydrazine-induced colon cancer in Sprague Dawley rats. Sprague Dawley rats were administered 1,2-dimethylhydrazine (40 mg/kg, i.p. once a week for 10 weeks) and a high-fat diet (HFD) for 20 weeks to induce colorectal cancer. APEE was administered at 125 mg/kg, 250 mg/kg, and 500 mg/kg for 20 weeks. At the end of the experiment, blood serum and organs were collected. DMH/HFD-induced rats had abnormal crypts and more aberrant crypt foci (ACF). APEE at a dose of 500 mg/kg improved the dysplastic state of the colon tissue and caused a 32% reduction in the total ACF. HFD increased adipocyte cell size, while 500 mg/kg APEE reduced it. HFD and DMH/HFD rats had elevated serum insulin and leptin levels. Moreover, UHPLC-QTOF-MS analysis revealed that APEE was rich in anti-cancer phytochemicals. This finding suggests that APEE has anti-cancer potential against HFD/DMH-induced CRC and anti-adipogenic and anti-obesity properties. MDPI 2023-03 Article PeerReviewed Subarmaniam, Tharani and Mahmad Rusli, Rusydatul Nabila and Perumal, Kokila Vani and Yong, Yoke Keong and Siti Hadizah and Othman, Fezah and Salem, Khaled and Shafie, Nurul Husna and Hasham, Rosnani and Yin, Khoo Boon and Abdul Kadir, Khairul Kamilah and Bahari, Hasnah and Zakaria, Zainul Amiruddin (2023) The potential chemopreventive effect of Andrographis paniculata on 1,2-dimethylhydrazine and high-fat-diet-induced colorectal cancer in Sprague Dawley rats. International Journal of Molecular Sciences, 24 (6). art. no. 5224. pp. 1-17. ISSN 1661-6596; ESSN: 1422-0067 https://www.mdpi.com/1422-0067/24/6/5224 10.3390/ijms24065224 |
| spellingShingle | Subarmaniam, Tharani Mahmad Rusli, Rusydatul Nabila Perumal, Kokila Vani Yong, Yoke Keong Siti Hadizah Othman, Fezah Salem, Khaled Shafie, Nurul Husna Hasham, Rosnani Yin, Khoo Boon Abdul Kadir, Khairul Kamilah Bahari, Hasnah Zakaria, Zainul Amiruddin The potential chemopreventive effect of Andrographis paniculata on 1,2-dimethylhydrazine and high-fat-diet-induced colorectal cancer in Sprague Dawley rats |
| title | The potential chemopreventive effect of Andrographis paniculata on 1,2-dimethylhydrazine and high-fat-diet-induced colorectal cancer in Sprague Dawley rats |
| title_full | The potential chemopreventive effect of Andrographis paniculata on 1,2-dimethylhydrazine and high-fat-diet-induced colorectal cancer in Sprague Dawley rats |
| title_fullStr | The potential chemopreventive effect of Andrographis paniculata on 1,2-dimethylhydrazine and high-fat-diet-induced colorectal cancer in Sprague Dawley rats |
| title_full_unstemmed | The potential chemopreventive effect of Andrographis paniculata on 1,2-dimethylhydrazine and high-fat-diet-induced colorectal cancer in Sprague Dawley rats |
| title_short | The potential chemopreventive effect of Andrographis paniculata on 1,2-dimethylhydrazine and high-fat-diet-induced colorectal cancer in Sprague Dawley rats |
| title_sort | potential chemopreventive effect of andrographis paniculata on 1,2-dimethylhydrazine and high-fat-diet-induced colorectal cancer in sprague dawley rats |
| url | http://psasir.upm.edu.my/id/eprint/109056/ http://psasir.upm.edu.my/id/eprint/109056/ http://psasir.upm.edu.my/id/eprint/109056/ |