Therapeutic potential of albumin nanoparticles encapsulated visnagin in MDA-MB-468 triple-negative breast cancer cells
Breast cancer is among the most recurrent malignancies, and its prevalence is rising. With only a few treatment options available, there is an immediate need to search for better alternatives. In this regard, nanotechnology has been applied to develop potential chemotherapeutic techniques, particula...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Published: |
MDPI
2023
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/109025/ |
| _version_ | 1848865264648585216 |
|---|---|
| author | Alsrhani, Abdullah Elderdery, Abozer Y. Alzahrani, Badr Alzerwi, Nasser A. N. Althobiti, Maryam Musleh Rayzah, Musaed Idrees, Bandar Elkhalifa, Ahmed M. E. Subbiah, Suresh K. Mok, Pooi Ling |
| author_facet | Alsrhani, Abdullah Elderdery, Abozer Y. Alzahrani, Badr Alzerwi, Nasser A. N. Althobiti, Maryam Musleh Rayzah, Musaed Idrees, Bandar Elkhalifa, Ahmed M. E. Subbiah, Suresh K. Mok, Pooi Ling |
| author_sort | Alsrhani, Abdullah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Breast cancer is among the most recurrent malignancies, and its prevalence is rising. With only a few treatment options available, there is an immediate need to search for better alternatives. In this regard, nanotechnology has been applied to develop potential chemotherapeutic techniques, particularly for cancer therapy. Specifically, albumin-based nanoparticles are a developing platform for the administration of diverse chemotherapy drugs owing to their biocompatibility and non-toxicity. Visnagin, a naturally derived furanochromone, treats cancers, epilepsy, angina, coughs, and inflammatory illnesses. In the current study, the synthesis and characterization of albumin visnagin (AV) nanoparticles (NPs) using a variety of techniques such as transmission electron microscopy, UV-visible, Fourier transform infrared, energy dispersive X-ray composition analysis, field emission scanning electron microscopy, photoluminescence, X-Ray diffraction, and dynamic light scattering analyses have been carried out. The MTT test, dual AO/EB, DCFH-DA, Annexin-V-FITC/PI, Propidium iodide staining techniques as well as analysis of apoptotic proteins, antioxidant enzymes, and PI3K/Akt/mTOR signaling analysis was performed to examine the NPs’ efficacy to suppress MDA-MB-468 cell lines. The NPs decreased cell viability increased the amount of ROS in the cells, disrupted membrane integrity, decreased the level of antioxidant enzymes, induced cell cycle arrest, and activated the PI3K/Akt/mTOR signaling cascade, ultimately leading to cell death. Thus, AV NPs possesses huge potential to be employed as a strong anticancer therapy alternative. |
| first_indexed | 2025-11-15T14:01:57Z |
| format | Article |
| id | upm-109025 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T14:01:57Z |
| publishDate | 2023 |
| publisher | MDPI |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1090252024-10-14T04:02:22Z http://psasir.upm.edu.my/id/eprint/109025/ Therapeutic potential of albumin nanoparticles encapsulated visnagin in MDA-MB-468 triple-negative breast cancer cells Alsrhani, Abdullah Elderdery, Abozer Y. Alzahrani, Badr Alzerwi, Nasser A. N. Althobiti, Maryam Musleh Rayzah, Musaed Idrees, Bandar Elkhalifa, Ahmed M. E. Subbiah, Suresh K. Mok, Pooi Ling Breast cancer is among the most recurrent malignancies, and its prevalence is rising. With only a few treatment options available, there is an immediate need to search for better alternatives. In this regard, nanotechnology has been applied to develop potential chemotherapeutic techniques, particularly for cancer therapy. Specifically, albumin-based nanoparticles are a developing platform for the administration of diverse chemotherapy drugs owing to their biocompatibility and non-toxicity. Visnagin, a naturally derived furanochromone, treats cancers, epilepsy, angina, coughs, and inflammatory illnesses. In the current study, the synthesis and characterization of albumin visnagin (AV) nanoparticles (NPs) using a variety of techniques such as transmission electron microscopy, UV-visible, Fourier transform infrared, energy dispersive X-ray composition analysis, field emission scanning electron microscopy, photoluminescence, X-Ray diffraction, and dynamic light scattering analyses have been carried out. The MTT test, dual AO/EB, DCFH-DA, Annexin-V-FITC/PI, Propidium iodide staining techniques as well as analysis of apoptotic proteins, antioxidant enzymes, and PI3K/Akt/mTOR signaling analysis was performed to examine the NPs’ efficacy to suppress MDA-MB-468 cell lines. The NPs decreased cell viability increased the amount of ROS in the cells, disrupted membrane integrity, decreased the level of antioxidant enzymes, induced cell cycle arrest, and activated the PI3K/Akt/mTOR signaling cascade, ultimately leading to cell death. Thus, AV NPs possesses huge potential to be employed as a strong anticancer therapy alternative. MDPI 2023-04-04 Article PeerReviewed Alsrhani, Abdullah and Elderdery, Abozer Y. and Alzahrani, Badr and Alzerwi, Nasser A. N. and Althobiti, Maryam Musleh and Rayzah, Musaed and Idrees, Bandar and Elkhalifa, Ahmed M. E. and Subbiah, Suresh K. and Mok, Pooi Ling (2023) Therapeutic potential of albumin nanoparticles encapsulated visnagin in MDA-MB-468 triple-negative breast cancer cells. Molecules, 28 (7). art. no. 3228. pp. 1-18. ISSN 1420-3049 https://www.mdpi.com/1420-3049/28/7/3228 10.3390/molecules28073228 |
| spellingShingle | Alsrhani, Abdullah Elderdery, Abozer Y. Alzahrani, Badr Alzerwi, Nasser A. N. Althobiti, Maryam Musleh Rayzah, Musaed Idrees, Bandar Elkhalifa, Ahmed M. E. Subbiah, Suresh K. Mok, Pooi Ling Therapeutic potential of albumin nanoparticles encapsulated visnagin in MDA-MB-468 triple-negative breast cancer cells |
| title | Therapeutic potential of albumin nanoparticles encapsulated visnagin in MDA-MB-468 triple-negative breast cancer cells |
| title_full | Therapeutic potential of albumin nanoparticles encapsulated visnagin in MDA-MB-468 triple-negative breast cancer cells |
| title_fullStr | Therapeutic potential of albumin nanoparticles encapsulated visnagin in MDA-MB-468 triple-negative breast cancer cells |
| title_full_unstemmed | Therapeutic potential of albumin nanoparticles encapsulated visnagin in MDA-MB-468 triple-negative breast cancer cells |
| title_short | Therapeutic potential of albumin nanoparticles encapsulated visnagin in MDA-MB-468 triple-negative breast cancer cells |
| title_sort | therapeutic potential of albumin nanoparticles encapsulated visnagin in mda-mb-468 triple-negative breast cancer cells |
| url | http://psasir.upm.edu.my/id/eprint/109025/ http://psasir.upm.edu.my/id/eprint/109025/ http://psasir.upm.edu.my/id/eprint/109025/ |