Molecular epidemiology of piliated pneumococcal isolates at a major tertiary hospital in the Klang Valley, Malaysia
Objective: To characterise a collection of pili-carrying and none pili-carrying pneumococcal isolates of clinical origin for serotypes, antibiotic resistance and genotype. Methods: In total, 42 clinical isolates were collected between October 2017 and December 2019. Those isolates were analysed...
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Published: |
Medknow Publications
2023
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/109008/ |
| _version_ | 1848865260392415232 |
|---|---|
| author | Dzaraly, Nurul Diana Mohd Desa, Mohd Nasir Muthanna, Abdulrahman Masri, Siti Norbaya Mohd Taib, Niazlin Zainal Baharin, Nurul Hana Sulaiman, Nurshahira Abdul Rahman, Nurul Asyikin Palanisamy, Navindra Kumari Suhaili, Zarizal Yusuff, Semiat Opeyemi Rahman, Nor Iza A. Tuan Soh, Tuan Suhaila Abdullah, Fatimah Haslina |
| author_facet | Dzaraly, Nurul Diana Mohd Desa, Mohd Nasir Muthanna, Abdulrahman Masri, Siti Norbaya Mohd Taib, Niazlin Zainal Baharin, Nurul Hana Sulaiman, Nurshahira Abdul Rahman, Nurul Asyikin Palanisamy, Navindra Kumari Suhaili, Zarizal Yusuff, Semiat Opeyemi Rahman, Nor Iza A. Tuan Soh, Tuan Suhaila Abdullah, Fatimah Haslina |
| author_sort | Dzaraly, Nurul Diana |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Objective: To characterise a collection of pili-carrying and none
pili-carrying pneumococcal isolates of clinical origin for serotypes,
antibiotic resistance and genotype.
Methods: In total, 42 clinical isolates were collected between
October 2017 and December 2019. Those isolates were analysed
for antimicrobial susceptibility, serotype distribution, detection of
pneumococcal virulence and pilus genes. Multilocus sequence typing
was performed only for piliated isolates, followed by phylogenetic
analysis.
Results: The common isolation sites among the pneumococcal
isolates were tracheal aspirate (28.6%), blood (26.2%), and sputum
(23.8%). Fifty percent isolates were resistant to erythromycin,
tetracycline (50.0%) and trimethoprim-sulfamethoxazole (43.0%).
The most frequent were serotypes 19F (28.6%), 6A/B (23.8%) and
19A (14.3%). Piliated isolates were detected in a small proportion
(33.3%); 64.3% were multidrug-resistant. ST320 was the prevalent
sequence type among the piliated isolates and genetically related
to the Pneumococcal Molecular Epidemiology Network clones
Taiwan19F-14 (CC271). In the phylogenetic analysis, some piliated
isolates showed a close association having similar ST320, carrying
serotype 19A and both pilus genes indicating their clonal spread.
Conclusions: Pneumococcal lineages of piliated isolates have been
globally disseminated and pili could have played a role in the spread
of antibiotic resistant clones. |
| first_indexed | 2025-11-15T14:01:53Z |
| format | Article |
| id | upm-109008 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T14:01:53Z |
| publishDate | 2023 |
| publisher | Medknow Publications |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1090082024-10-14T07:04:39Z http://psasir.upm.edu.my/id/eprint/109008/ Molecular epidemiology of piliated pneumococcal isolates at a major tertiary hospital in the Klang Valley, Malaysia Dzaraly, Nurul Diana Mohd Desa, Mohd Nasir Muthanna, Abdulrahman Masri, Siti Norbaya Mohd Taib, Niazlin Zainal Baharin, Nurul Hana Sulaiman, Nurshahira Abdul Rahman, Nurul Asyikin Palanisamy, Navindra Kumari Suhaili, Zarizal Yusuff, Semiat Opeyemi Rahman, Nor Iza A. Tuan Soh, Tuan Suhaila Abdullah, Fatimah Haslina Objective: To characterise a collection of pili-carrying and none pili-carrying pneumococcal isolates of clinical origin for serotypes, antibiotic resistance and genotype. Methods: In total, 42 clinical isolates were collected between October 2017 and December 2019. Those isolates were analysed for antimicrobial susceptibility, serotype distribution, detection of pneumococcal virulence and pilus genes. Multilocus sequence typing was performed only for piliated isolates, followed by phylogenetic analysis. Results: The common isolation sites among the pneumococcal isolates were tracheal aspirate (28.6%), blood (26.2%), and sputum (23.8%). Fifty percent isolates were resistant to erythromycin, tetracycline (50.0%) and trimethoprim-sulfamethoxazole (43.0%). The most frequent were serotypes 19F (28.6%), 6A/B (23.8%) and 19A (14.3%). Piliated isolates were detected in a small proportion (33.3%); 64.3% were multidrug-resistant. ST320 was the prevalent sequence type among the piliated isolates and genetically related to the Pneumococcal Molecular Epidemiology Network clones Taiwan19F-14 (CC271). In the phylogenetic analysis, some piliated isolates showed a close association having similar ST320, carrying serotype 19A and both pilus genes indicating their clonal spread. Conclusions: Pneumococcal lineages of piliated isolates have been globally disseminated and pili could have played a role in the spread of antibiotic resistant clones. Medknow Publications 2023-02 Article PeerReviewed Dzaraly, Nurul Diana and Mohd Desa, Mohd Nasir and Muthanna, Abdulrahman and Masri, Siti Norbaya and Mohd Taib, Niazlin and Zainal Baharin, Nurul Hana and Sulaiman, Nurshahira and Abdul Rahman, Nurul Asyikin and Palanisamy, Navindra Kumari and Suhaili, Zarizal and Yusuff, Semiat Opeyemi and Rahman, Nor Iza A. and Tuan Soh, Tuan Suhaila and Abdullah, Fatimah Haslina (2023) Molecular epidemiology of piliated pneumococcal isolates at a major tertiary hospital in the Klang Valley, Malaysia. Asian Pacific Journal of Tropical Medicine, 16 (2). pp. 80-88. ISSN 1995-7645; ESSN: 2352-4146 https://journals.lww.com/aptm/fulltext/2023/16020/molecular_epidemiology_of_piliated_pneumococcal.6.aspx 10.4103/1995-7645.370150 |
| spellingShingle | Dzaraly, Nurul Diana Mohd Desa, Mohd Nasir Muthanna, Abdulrahman Masri, Siti Norbaya Mohd Taib, Niazlin Zainal Baharin, Nurul Hana Sulaiman, Nurshahira Abdul Rahman, Nurul Asyikin Palanisamy, Navindra Kumari Suhaili, Zarizal Yusuff, Semiat Opeyemi Rahman, Nor Iza A. Tuan Soh, Tuan Suhaila Abdullah, Fatimah Haslina Molecular epidemiology of piliated pneumococcal isolates at a major tertiary hospital in the Klang Valley, Malaysia |
| title | Molecular epidemiology of piliated pneumococcal isolates at a major tertiary hospital in the Klang Valley, Malaysia |
| title_full | Molecular epidemiology of piliated pneumococcal isolates at a major tertiary hospital in the Klang Valley, Malaysia |
| title_fullStr | Molecular epidemiology of piliated pneumococcal isolates at a major tertiary hospital in the Klang Valley, Malaysia |
| title_full_unstemmed | Molecular epidemiology of piliated pneumococcal isolates at a major tertiary hospital in the Klang Valley, Malaysia |
| title_short | Molecular epidemiology of piliated pneumococcal isolates at a major tertiary hospital in the Klang Valley, Malaysia |
| title_sort | molecular epidemiology of piliated pneumococcal isolates at a major tertiary hospital in the klang valley, malaysia |
| url | http://psasir.upm.edu.my/id/eprint/109008/ http://psasir.upm.edu.my/id/eprint/109008/ http://psasir.upm.edu.my/id/eprint/109008/ |