In vitro applicability of mixed soy lecithin-based liposomes with added several lipophilic agents as novel delivery systems for delivery of quercetin
The study used highly lipophilic compounds to evaluate membrane physical and constitutional changes and vesicle stabilization of mixed soy lecithin (ML)-based liposomes. The in vitro bio-accessibility of novel quercetin (QU)-loaded ML-based liposomes as a function of particle size, polydispersity in...
| Main Authors: | , , , , |
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| Format: | Article |
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Taylor and Francis
2023
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| Online Access: | http://psasir.upm.edu.my/id/eprint/108730/ |
| _version_ | 1848865199698739200 |
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| author | Toopkanloo, Sahar Pakbaten Tan, Tai Boon Cheong, Ling-Zhi Liu, Yuanfa Tan, Chin Ping |
| author_facet | Toopkanloo, Sahar Pakbaten Tan, Tai Boon Cheong, Ling-Zhi Liu, Yuanfa Tan, Chin Ping |
| author_sort | Toopkanloo, Sahar Pakbaten |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The study used highly lipophilic compounds to evaluate membrane physical and constitutional changes and vesicle stabilization of mixed soy lecithin (ML)-based liposomes. The in vitro bio-accessibility of novel quercetin (QU)-loaded ML-based liposomes as a function of particle size, polydispersity index (PDI), zeta potential (ZP), different membrane stabilizers, membrane fluidity and encapsulation efficacy (EE) was examined. Three different membrane systems, empty ML-based liposomes (E-LPs), squalene (SQ) loaded LPs (SQ-LPs), and SQ and a combination of β-sitosterol (βS) and stigmasterol (ST) loaded LPs (SQ-βS-ST-LPs), were developed based on optimized formulations using an extrusion method and used to encapsulate QU. The applicability of the ML-based liposomal carriers was tested by means of an in-vitro digestion procedure by applying a 2-phase static in- vitro model of the stomach and small intestine allowing for measurement of the bio-accessibility of ingested QU. Stability comparison of the empty liposomal particles, SQ-LPs and SQ-βS-ST-LPs particles was carried out to determine the most appropriate form of QU-loaded ML-based liposomal formulation. Different formulations were observed to possess different amounts of bio-accessible QU, indicating that membrane additives had profound effect on micellization of QU during Gastro-Intestinal Tract (GIT). Bivariate correlation analysis indicated that the bio-accessibility and EE of QU were strongly linearly correlated (R2 = 0.989), suggesting that QU bio-accessibility depended strongly on the retaining of QU in the ML bilayer, and entrapment capacity of the system. Our results paint a consistent picture in which the vesicle integrity, membrane fluidity and membrane-stabilizing function varied upon changing the membrane lipid composition in the ML-based-liposomes. |
| first_indexed | 2025-11-15T14:00:55Z |
| format | Article |
| id | upm-108730 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T14:00:55Z |
| publishDate | 2023 |
| publisher | Taylor and Francis |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1087302024-10-02T02:48:18Z http://psasir.upm.edu.my/id/eprint/108730/ In vitro applicability of mixed soy lecithin-based liposomes with added several lipophilic agents as novel delivery systems for delivery of quercetin Toopkanloo, Sahar Pakbaten Tan, Tai Boon Cheong, Ling-Zhi Liu, Yuanfa Tan, Chin Ping The study used highly lipophilic compounds to evaluate membrane physical and constitutional changes and vesicle stabilization of mixed soy lecithin (ML)-based liposomes. The in vitro bio-accessibility of novel quercetin (QU)-loaded ML-based liposomes as a function of particle size, polydispersity index (PDI), zeta potential (ZP), different membrane stabilizers, membrane fluidity and encapsulation efficacy (EE) was examined. Three different membrane systems, empty ML-based liposomes (E-LPs), squalene (SQ) loaded LPs (SQ-LPs), and SQ and a combination of β-sitosterol (βS) and stigmasterol (ST) loaded LPs (SQ-βS-ST-LPs), were developed based on optimized formulations using an extrusion method and used to encapsulate QU. The applicability of the ML-based liposomal carriers was tested by means of an in-vitro digestion procedure by applying a 2-phase static in- vitro model of the stomach and small intestine allowing for measurement of the bio-accessibility of ingested QU. Stability comparison of the empty liposomal particles, SQ-LPs and SQ-βS-ST-LPs particles was carried out to determine the most appropriate form of QU-loaded ML-based liposomal formulation. Different formulations were observed to possess different amounts of bio-accessible QU, indicating that membrane additives had profound effect on micellization of QU during Gastro-Intestinal Tract (GIT). Bivariate correlation analysis indicated that the bio-accessibility and EE of QU were strongly linearly correlated (R2 = 0.989), suggesting that QU bio-accessibility depended strongly on the retaining of QU in the ML bilayer, and entrapment capacity of the system. Our results paint a consistent picture in which the vesicle integrity, membrane fluidity and membrane-stabilizing function varied upon changing the membrane lipid composition in the ML-based-liposomes. Taylor and Francis 2023-12-16 Article PeerReviewed Toopkanloo, Sahar Pakbaten and Tan, Tai Boon and Cheong, Ling-Zhi and Liu, Yuanfa and Tan, Chin Ping (2023) In vitro applicability of mixed soy lecithin-based liposomes with added several lipophilic agents as novel delivery systems for delivery of quercetin. Journal of Dispersion Science And Technology, 44 (7). 1269 - 1277. ISSN 0193-2691; ESSN: 1532-2351 https://www.tandfonline.com/doi/abs/10.1080/01932691.2021.2013865 10.1080/01932691.2021.2013865 |
| spellingShingle | Toopkanloo, Sahar Pakbaten Tan, Tai Boon Cheong, Ling-Zhi Liu, Yuanfa Tan, Chin Ping In vitro applicability of mixed soy lecithin-based liposomes with added several lipophilic agents as novel delivery systems for delivery of quercetin |
| title | In vitro applicability of mixed soy lecithin-based liposomes with added several lipophilic agents as novel delivery systems for delivery of quercetin |
| title_full | In vitro applicability of mixed soy lecithin-based liposomes with added several lipophilic agents as novel delivery systems for delivery of quercetin |
| title_fullStr | In vitro applicability of mixed soy lecithin-based liposomes with added several lipophilic agents as novel delivery systems for delivery of quercetin |
| title_full_unstemmed | In vitro applicability of mixed soy lecithin-based liposomes with added several lipophilic agents as novel delivery systems for delivery of quercetin |
| title_short | In vitro applicability of mixed soy lecithin-based liposomes with added several lipophilic agents as novel delivery systems for delivery of quercetin |
| title_sort | in vitro applicability of mixed soy lecithin-based liposomes with added several lipophilic agents as novel delivery systems for delivery of quercetin |
| url | http://psasir.upm.edu.my/id/eprint/108730/ http://psasir.upm.edu.my/id/eprint/108730/ http://psasir.upm.edu.my/id/eprint/108730/ |