Probiotics bacteria as quorum sensing degrader control of aeromonas pathogenicity in cultured red hybrid tilapia
Quorum sensing (QS) is the interaction of bacteria cell-to-cell to regulate various bacterial functions, including bacterial virulence. It has been suggested that QS interruption is an anti-infective strategy to combat diseases in aquaculture. This research was conducted to isolate the potential pro...
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| Format: | Article |
| Language: | English |
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Agency for Marine and Fisheries Research and Development
2023
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| Online Access: | http://psasir.upm.edu.my/id/eprint/108713/ http://psasir.upm.edu.my/id/eprint/108713/1/Probiotics%20bacteria%20as%20quorum%20sensing%20degrader%20control.pdf.crdownload |
| _version_ | 1848865197530284032 |
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| author | Omar, Noor Syazwani Emilia, Sharifah Noor Danish-Daniel, Muhd Iehata, Shumpei Mohd Ikhsan, Natrah Fatin |
| author_facet | Omar, Noor Syazwani Emilia, Sharifah Noor Danish-Daniel, Muhd Iehata, Shumpei Mohd Ikhsan, Natrah Fatin |
| author_sort | Omar, Noor Syazwani |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Quorum sensing (QS) is the interaction of bacteria cell-to-cell to regulate various bacterial functions, including bacterial virulence. It has been suggested that QS interruption is an anti-infective strategy to combat diseases in aquaculture. This research was conducted to isolate the potential probiotics bacteria as QS degrader from the fish gut and investigate its efficiency in reducing Aeromonas hydrophila pathogenicity by in vitro and in vivo assay. In this study, three isolates from the fish gut were able to degrade N-acyl homoserine lactone (AHL), one of the QS signals of A. hydrophila. Based on 16S rDNA sequence analysis, isolate CPi12 was identified as Klebsiella sp., CBa5 and CBa7 were identified as Enterobacter tabaci. These isolates were co-cultured with A.hydrophila for five days. Results revealed that all isolates could decrease the AHL production of A. hydrophila but did not affect the growth of the pathogen. In vivo assay results showed that isolate CPi12 reduced the pathogenicity of A.hydrophila against tilapia with a significantly higher survival rate (p<0.05) of fish in the CPi12-fed group than the control group. Meanwhile, isolate CBa7 was significant (p<0.05) for growth performance, including specific growth rate (SGR), weight gain (WG), feed conversion ratio (FCR), mean weight gain (MWG), and average daily growth (ADG) among whole treatment and control group. These results displayed that the probiotics bacteria as QS degrader isolated from the fish gut could control the pathogenicity of Aeromonas hydrophila. It has been proposed that QS degrader bacteria might be an alternative solution for disease control. |
| first_indexed | 2025-11-15T14:00:53Z |
| format | Article |
| id | upm-108713 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:00:53Z |
| publishDate | 2023 |
| publisher | Agency for Marine and Fisheries Research and Development |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1087132024-10-11T08:37:54Z http://psasir.upm.edu.my/id/eprint/108713/ Probiotics bacteria as quorum sensing degrader control of aeromonas pathogenicity in cultured red hybrid tilapia Omar, Noor Syazwani Emilia, Sharifah Noor Danish-Daniel, Muhd Iehata, Shumpei Mohd Ikhsan, Natrah Fatin Quorum sensing (QS) is the interaction of bacteria cell-to-cell to regulate various bacterial functions, including bacterial virulence. It has been suggested that QS interruption is an anti-infective strategy to combat diseases in aquaculture. This research was conducted to isolate the potential probiotics bacteria as QS degrader from the fish gut and investigate its efficiency in reducing Aeromonas hydrophila pathogenicity by in vitro and in vivo assay. In this study, three isolates from the fish gut were able to degrade N-acyl homoserine lactone (AHL), one of the QS signals of A. hydrophila. Based on 16S rDNA sequence analysis, isolate CPi12 was identified as Klebsiella sp., CBa5 and CBa7 were identified as Enterobacter tabaci. These isolates were co-cultured with A.hydrophila for five days. Results revealed that all isolates could decrease the AHL production of A. hydrophila but did not affect the growth of the pathogen. In vivo assay results showed that isolate CPi12 reduced the pathogenicity of A.hydrophila against tilapia with a significantly higher survival rate (p<0.05) of fish in the CPi12-fed group than the control group. Meanwhile, isolate CBa7 was significant (p<0.05) for growth performance, including specific growth rate (SGR), weight gain (WG), feed conversion ratio (FCR), mean weight gain (MWG), and average daily growth (ADG) among whole treatment and control group. These results displayed that the probiotics bacteria as QS degrader isolated from the fish gut could control the pathogenicity of Aeromonas hydrophila. It has been proposed that QS degrader bacteria might be an alternative solution for disease control. Agency for Marine and Fisheries Research and Development 2023-06-30 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/108713/1/Probiotics%20bacteria%20as%20quorum%20sensing%20degrader%20control.pdf.crdownload Omar, Noor Syazwani and Emilia, Sharifah Noor and Danish-Daniel, Muhd and Iehata, Shumpei and Mohd Ikhsan, Natrah Fatin (2023) Probiotics bacteria as quorum sensing degrader control of aeromonas pathogenicity in cultured red hybrid tilapia. Indonesian Aquaculture Journal, 18 (1). pp. 1-15. ISSN 0215-0883; ESSN: 2502-6577 http://ejournal-balitbang.kkp.go.id/index.php/iaj/article/view/11887 10.15578/iaj.18.1.2023.1-15 |
| spellingShingle | Omar, Noor Syazwani Emilia, Sharifah Noor Danish-Daniel, Muhd Iehata, Shumpei Mohd Ikhsan, Natrah Fatin Probiotics bacteria as quorum sensing degrader control of aeromonas pathogenicity in cultured red hybrid tilapia |
| title | Probiotics bacteria as quorum sensing degrader control of aeromonas pathogenicity in cultured red hybrid tilapia |
| title_full | Probiotics bacteria as quorum sensing degrader control of aeromonas pathogenicity in cultured red hybrid tilapia |
| title_fullStr | Probiotics bacteria as quorum sensing degrader control of aeromonas pathogenicity in cultured red hybrid tilapia |
| title_full_unstemmed | Probiotics bacteria as quorum sensing degrader control of aeromonas pathogenicity in cultured red hybrid tilapia |
| title_short | Probiotics bacteria as quorum sensing degrader control of aeromonas pathogenicity in cultured red hybrid tilapia |
| title_sort | probiotics bacteria as quorum sensing degrader control of aeromonas pathogenicity in cultured red hybrid tilapia |
| url | http://psasir.upm.edu.my/id/eprint/108713/ http://psasir.upm.edu.my/id/eprint/108713/ http://psasir.upm.edu.my/id/eprint/108713/ http://psasir.upm.edu.my/id/eprint/108713/1/Probiotics%20bacteria%20as%20quorum%20sensing%20degrader%20control.pdf.crdownload |