Ultrasound-assisted extraction of antioxidants from Melastoma malabathricum Linn.: modeling and optimization using Box–Behnken design
This study presents modeling and optimization of ultrasound-assisted extraction (UAE) of Melastoma malabathricum with the objective of evaluating its phytochemical properties. This one-factor-at-a-time (OFAT) procedure was conducted to screen for optimization variables whose domains included extract...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI
2023
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| Online Access: | http://psasir.upm.edu.my/id/eprint/108376/ http://psasir.upm.edu.my/id/eprint/108376/1/108376.pdf |
| _version_ | 1848865145951879168 |
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| author | Hosni, Suzziyana Gani, Siti Salwa Abd Orsat, Valérie Hassan, Masriana Abdullah, Sumaiyah |
| author_facet | Hosni, Suzziyana Gani, Siti Salwa Abd Orsat, Valérie Hassan, Masriana Abdullah, Sumaiyah |
| author_sort | Hosni, Suzziyana |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | This study presents modeling and optimization of ultrasound-assisted extraction (UAE) of Melastoma malabathricum with the objective of evaluating its phytochemical properties. This one-factor-at-a-time (OFAT) procedure was conducted to screen for optimization variables whose domains included extraction temperature (XET), ultrasonic time (XUT), solvent concentration (XSC), and sample-to-liquid ratio (XSLR). Response surface methodology (RSM) coupled with Box–Behnken design (BBD) was applied to establish optimum conditions for maximum antioxidant extraction. Modeling and optimization conditions of UAE at 37 kHz, XET 32 °C for XUT 16 min and dissolved in an XSC 70% ethanol concentration at a XSLR 1:10 ratio yielded scavenging effects on 2,2-diphenyl-1-picryl-hydrazyl (DPPH) at 96% ± 1.48 and recorded values of total phenolic content (TPC) and total flavonoid content (TFC) at 803.456 ± 32.48 mg GAE (gallic acid equivalents)/g, and 102.972 ± 2.51 mg QE (quercetin equivalents)/g, respectively. The presence of high flavonoid compounds was verified using TWIMS-QTOFMS. Chromatic evaluation of phytochemicals using gas chromatography–mass spectrometry (GC–MS) revealed the presence of 14 phytocompounds widely documented to play significant roles in human health. This study provides a comparative evaluation with other studies and may be used for validation of the species’ potential for its much-acclaimed medicinal and cosmeceutical uses. |
| first_indexed | 2025-11-15T14:00:03Z |
| format | Article |
| id | upm-108376 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:00:03Z |
| publishDate | 2023 |
| publisher | MDPI |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1083762025-06-25T06:58:27Z http://psasir.upm.edu.my/id/eprint/108376/ Ultrasound-assisted extraction of antioxidants from Melastoma malabathricum Linn.: modeling and optimization using Box–Behnken design Hosni, Suzziyana Gani, Siti Salwa Abd Orsat, Valérie Hassan, Masriana Abdullah, Sumaiyah This study presents modeling and optimization of ultrasound-assisted extraction (UAE) of Melastoma malabathricum with the objective of evaluating its phytochemical properties. This one-factor-at-a-time (OFAT) procedure was conducted to screen for optimization variables whose domains included extraction temperature (XET), ultrasonic time (XUT), solvent concentration (XSC), and sample-to-liquid ratio (XSLR). Response surface methodology (RSM) coupled with Box–Behnken design (BBD) was applied to establish optimum conditions for maximum antioxidant extraction. Modeling and optimization conditions of UAE at 37 kHz, XET 32 °C for XUT 16 min and dissolved in an XSC 70% ethanol concentration at a XSLR 1:10 ratio yielded scavenging effects on 2,2-diphenyl-1-picryl-hydrazyl (DPPH) at 96% ± 1.48 and recorded values of total phenolic content (TPC) and total flavonoid content (TFC) at 803.456 ± 32.48 mg GAE (gallic acid equivalents)/g, and 102.972 ± 2.51 mg QE (quercetin equivalents)/g, respectively. The presence of high flavonoid compounds was verified using TWIMS-QTOFMS. Chromatic evaluation of phytochemicals using gas chromatography–mass spectrometry (GC–MS) revealed the presence of 14 phytocompounds widely documented to play significant roles in human health. This study provides a comparative evaluation with other studies and may be used for validation of the species’ potential for its much-acclaimed medicinal and cosmeceutical uses. MDPI 2023-01 Article PeerReviewed text en cc_by_4 http://psasir.upm.edu.my/id/eprint/108376/1/108376.pdf Hosni, Suzziyana and Gani, Siti Salwa Abd and Orsat, Valérie and Hassan, Masriana and Abdullah, Sumaiyah (2023) Ultrasound-assisted extraction of antioxidants from Melastoma malabathricum Linn.: modeling and optimization using Box–Behnken design. Molecules, 28 (2). art. no. 487. ISSN 1420-3049; eISSN: 1420-3049 https://www.mdpi.com/1420-3049/28/2/487 10.3390/molecules28020487 |
| spellingShingle | Hosni, Suzziyana Gani, Siti Salwa Abd Orsat, Valérie Hassan, Masriana Abdullah, Sumaiyah Ultrasound-assisted extraction of antioxidants from Melastoma malabathricum Linn.: modeling and optimization using Box–Behnken design |
| title | Ultrasound-assisted extraction of antioxidants from Melastoma malabathricum Linn.: modeling and optimization using Box–Behnken design |
| title_full | Ultrasound-assisted extraction of antioxidants from Melastoma malabathricum Linn.: modeling and optimization using Box–Behnken design |
| title_fullStr | Ultrasound-assisted extraction of antioxidants from Melastoma malabathricum Linn.: modeling and optimization using Box–Behnken design |
| title_full_unstemmed | Ultrasound-assisted extraction of antioxidants from Melastoma malabathricum Linn.: modeling and optimization using Box–Behnken design |
| title_short | Ultrasound-assisted extraction of antioxidants from Melastoma malabathricum Linn.: modeling and optimization using Box–Behnken design |
| title_sort | ultrasound-assisted extraction of antioxidants from melastoma malabathricum linn.: modeling and optimization using box–behnken design |
| url | http://psasir.upm.edu.my/id/eprint/108376/ http://psasir.upm.edu.my/id/eprint/108376/ http://psasir.upm.edu.my/id/eprint/108376/ http://psasir.upm.edu.my/id/eprint/108376/1/108376.pdf |