Identification of potential biomarkers and metabolic changes in the serum of breast lump patients among Kelantanese based on 1H NMR metabolomics
This case-control study, conducted at the Hospital USM BestARi unit, aimed to identify the serum metabolic fingerprint among individuals with breast lumps and healthy controls, and to discover potential biomarkers. Serum samples from healthy controls, benign breast lump patients, and malignant breas...
| Main Authors: | , , , , , |
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| Format: | Article |
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HH Publisher
2023
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| Online Access: | http://psasir.upm.edu.my/id/eprint/108248/ |
| _version_ | 1848865112610308096 |
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| author | Zulkefly, Ahmad Abas, Faridah Wan Azman, Wan Norlina Omar, Julia Ab Mutalib, Nurul Syakima Syamsumir, Desy Fitrya |
| author_facet | Zulkefly, Ahmad Abas, Faridah Wan Azman, Wan Norlina Omar, Julia Ab Mutalib, Nurul Syakima Syamsumir, Desy Fitrya |
| author_sort | Zulkefly, Ahmad |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | This case-control study, conducted at the Hospital USM BestARi unit, aimed to identify the serum metabolic fingerprint among individuals with breast lumps and healthy controls, and to discover potential biomarkers. Serum samples from healthy controls, benign breast lump patients, and malignant breast lump patients were analyzed using proton nuclear magnetic resonance spectroscopy (1H NMR). A multivariate data analysis approach was employed, with the OPLS-DA and clustered heat map techniques effectively differentiating between the three groups. The study revealed significant metabolite variations across the groups and proposed D-glucose, glycerol, and glycine as potential biomarkers for breast cancer diagnosis. Metabolic pathways such as alanine, aspartate, glutamate metabolism, and glycine, serine, and threonine metabolism were implicated. The metabolomics approach coupled with multivariate analysis successfully identified key metabolites leading to group separation and suggested altered metabolic pathways. However, further research and integration with other ‘omics’ technologies are necessary for clinical translation. |
| first_indexed | 2025-11-15T13:59:32Z |
| format | Article |
| id | upm-108248 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:59:32Z |
| publishDate | 2023 |
| publisher | HH Publisher |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1082482024-09-11T03:20:22Z http://psasir.upm.edu.my/id/eprint/108248/ Identification of potential biomarkers and metabolic changes in the serum of breast lump patients among Kelantanese based on 1H NMR metabolomics Zulkefly, Ahmad Abas, Faridah Wan Azman, Wan Norlina Omar, Julia Ab Mutalib, Nurul Syakima Syamsumir, Desy Fitrya This case-control study, conducted at the Hospital USM BestARi unit, aimed to identify the serum metabolic fingerprint among individuals with breast lumps and healthy controls, and to discover potential biomarkers. Serum samples from healthy controls, benign breast lump patients, and malignant breast lump patients were analyzed using proton nuclear magnetic resonance spectroscopy (1H NMR). A multivariate data analysis approach was employed, with the OPLS-DA and clustered heat map techniques effectively differentiating between the three groups. The study revealed significant metabolite variations across the groups and proposed D-glucose, glycerol, and glycine as potential biomarkers for breast cancer diagnosis. Metabolic pathways such as alanine, aspartate, glutamate metabolism, and glycine, serine, and threonine metabolism were implicated. The metabolomics approach coupled with multivariate analysis successfully identified key metabolites leading to group separation and suggested altered metabolic pathways. However, further research and integration with other ‘omics’ technologies are necessary for clinical translation. HH Publisher 2023 Article PeerReviewed Zulkefly, Ahmad and Abas, Faridah and Wan Azman, Wan Norlina and Omar, Julia and Ab Mutalib, Nurul Syakima and Syamsumir, Desy Fitrya (2023) Identification of potential biomarkers and metabolic changes in the serum of breast lump patients among Kelantanese based on 1H NMR metabolomics. Progress in Microbes and Molecular Biology, 6 (1). art. no. a0000392. pp. 1-18. ISSN 2637-1049 https://journals.hh-publisher.com/index.php/pmmb/article/view/922 10.36877/pmmb.a0000392 |
| spellingShingle | Zulkefly, Ahmad Abas, Faridah Wan Azman, Wan Norlina Omar, Julia Ab Mutalib, Nurul Syakima Syamsumir, Desy Fitrya Identification of potential biomarkers and metabolic changes in the serum of breast lump patients among Kelantanese based on 1H NMR metabolomics |
| title | Identification of potential biomarkers and metabolic changes in the serum of breast lump patients among Kelantanese based on 1H NMR metabolomics |
| title_full | Identification of potential biomarkers and metabolic changes in the serum of breast lump patients among Kelantanese based on 1H NMR metabolomics |
| title_fullStr | Identification of potential biomarkers and metabolic changes in the serum of breast lump patients among Kelantanese based on 1H NMR metabolomics |
| title_full_unstemmed | Identification of potential biomarkers and metabolic changes in the serum of breast lump patients among Kelantanese based on 1H NMR metabolomics |
| title_short | Identification of potential biomarkers and metabolic changes in the serum of breast lump patients among Kelantanese based on 1H NMR metabolomics |
| title_sort | identification of potential biomarkers and metabolic changes in the serum of breast lump patients among kelantanese based on 1h nmr metabolomics |
| url | http://psasir.upm.edu.my/id/eprint/108248/ http://psasir.upm.edu.my/id/eprint/108248/ http://psasir.upm.edu.my/id/eprint/108248/ |