Allozyme variations of Trichoderma harzianum and its taxonomic implications
Electrophoretic variation of nine allozyme systems encoded by 14 gene loci were studied on 47 isolates from 3 species of Trichoderma namely, T. harzianum, T. aureoviride and T. longibrachiatum. Polyacrylamide gel electrophoresis was used to investigate the taxonomic circumscription of T. harzianum p...
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American-Eurasian Network for Scientific Information
2007
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| Online Access: | http://psasir.upm.edu.my/id/eprint/10822/ http://psasir.upm.edu.my/id/eprint/10822/1/Allozyme%20variations%20of%20Trichoderma%20harzianum%20and%20its%20taxonomic%20implications.pdf |
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| author | Siddiquee, Md. Shafiquzzaman Abdullah, Faridah Tan, Soon Guan Leng, Min See |
| author_facet | Siddiquee, Md. Shafiquzzaman Abdullah, Faridah Tan, Soon Guan Leng, Min See |
| author_sort | Siddiquee, Md. Shafiquzzaman |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Electrophoretic variation of nine allozyme systems encoded by 14 gene loci were studied on 47 isolates from 3 species of Trichoderma namely, T. harzianum, T. aureoviride and T. longibrachiatum. Polyacrylamide gel electrophoresis was used to investigate the taxonomic circumscription of T. harzianum populations and to evaluate the levels of genetic variations and the population structure. The Level of genetic variations in T. harzianum populations were moderately high (P= 57.10%, A= 0.7857, Ap= 0.60714 and H = 0.1542) compared to E T. aureoviride and T. longibrachiatum. The genetic variation attributable to differences among populations was 7.857%. The mean gene flow among populations was Nm = 1.3351. Genetic identities (I) ranged from 0.9397 to 0.9642 with a mean of 0.94846. Outcrossing rates based on fixation indices average (t) was 0.2334. Nevertheless, the alleles for α-EST-b showed a very low frequency of 0.0400. The polymorphic locus of MDH1 was of the fast allele of T. harzianum, MD1-a, was prevalent in T. aureoviride and T. longibrachiatum populations. Using a UPGMA cluster analysis, T. harzianum and T. longibrachiatum populations were totally separated in these cluster except T. aureoviride populations. T. harzianum presents high levels of genetic diversity compared with other Trichoderma species. |
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| format | Article |
| id | upm-10822 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T07:44:24Z |
| publishDate | 2007 |
| publisher | American-Eurasian Network for Scientific Information |
| recordtype | eprints |
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| spelling | upm-108222018-09-21T01:52:00Z http://psasir.upm.edu.my/id/eprint/10822/ Allozyme variations of Trichoderma harzianum and its taxonomic implications Siddiquee, Md. Shafiquzzaman Abdullah, Faridah Tan, Soon Guan Leng, Min See Electrophoretic variation of nine allozyme systems encoded by 14 gene loci were studied on 47 isolates from 3 species of Trichoderma namely, T. harzianum, T. aureoviride and T. longibrachiatum. Polyacrylamide gel electrophoresis was used to investigate the taxonomic circumscription of T. harzianum populations and to evaluate the levels of genetic variations and the population structure. The Level of genetic variations in T. harzianum populations were moderately high (P= 57.10%, A= 0.7857, Ap= 0.60714 and H = 0.1542) compared to E T. aureoviride and T. longibrachiatum. The genetic variation attributable to differences among populations was 7.857%. The mean gene flow among populations was Nm = 1.3351. Genetic identities (I) ranged from 0.9397 to 0.9642 with a mean of 0.94846. Outcrossing rates based on fixation indices average (t) was 0.2334. Nevertheless, the alleles for α-EST-b showed a very low frequency of 0.0400. The polymorphic locus of MDH1 was of the fast allele of T. harzianum, MD1-a, was prevalent in T. aureoviride and T. longibrachiatum populations. Using a UPGMA cluster analysis, T. harzianum and T. longibrachiatum populations were totally separated in these cluster except T. aureoviride populations. T. harzianum presents high levels of genetic diversity compared with other Trichoderma species. American-Eurasian Network for Scientific Information 2007 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/10822/1/Allozyme%20variations%20of%20Trichoderma%20harzianum%20and%20its%20taxonomic%20implications.pdf Siddiquee, Md. Shafiquzzaman and Abdullah, Faridah and Tan, Soon Guan and Leng, Min See (2007) Allozyme variations of Trichoderma harzianum and its taxonomic implications. Australian Journal of Basic and Applied Sciences, 1 (1). pp. 30-37. ISSN 1991-8178 http://ajbasweb.com/old/ajbas_jan-march_2007.html |
| spellingShingle | Siddiquee, Md. Shafiquzzaman Abdullah, Faridah Tan, Soon Guan Leng, Min See Allozyme variations of Trichoderma harzianum and its taxonomic implications |
| title | Allozyme variations of Trichoderma harzianum and its taxonomic implications |
| title_full | Allozyme variations of Trichoderma harzianum and its taxonomic implications |
| title_fullStr | Allozyme variations of Trichoderma harzianum and its taxonomic implications |
| title_full_unstemmed | Allozyme variations of Trichoderma harzianum and its taxonomic implications |
| title_short | Allozyme variations of Trichoderma harzianum and its taxonomic implications |
| title_sort | allozyme variations of trichoderma harzianum and its taxonomic implications |
| url | http://psasir.upm.edu.my/id/eprint/10822/ http://psasir.upm.edu.my/id/eprint/10822/ http://psasir.upm.edu.my/id/eprint/10822/1/Allozyme%20variations%20of%20Trichoderma%20harzianum%20and%20its%20taxonomic%20implications.pdf |