Genomic analysis and biological characterization of a novel Schitoviridae phage infecting Vibrio alginolyticus
Abstract: Vibrio alginolyticus is a Gram-negative bacterium commonly associated with mackerel poisoning. A bacteriophage that specifically targets and lyses this bacterium could be employed as a biocontrol agent for treating the bacterial infection or improving the shelf-life of mackerel products. H...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
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Springer
2023
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| Online Access: | http://psasir.upm.edu.my/id/eprint/108099/ |
| _version_ | 1848865072822091776 |
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| author | Tajuddin, Sofiah Khan, Asif M. Li, Chuin Chong Wong, Chuan Loo Jia, Sen Tan Ina-Salwany, Md Yasin Lau, Han Yih Kok, Lian Ho Mariatulqabtiah, Abdul Razak Wen, Siang Tan |
| author_facet | Tajuddin, Sofiah Khan, Asif M. Li, Chuin Chong Wong, Chuan Loo Jia, Sen Tan Ina-Salwany, Md Yasin Lau, Han Yih Kok, Lian Ho Mariatulqabtiah, Abdul Razak Wen, Siang Tan |
| author_sort | Tajuddin, Sofiah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Abstract: Vibrio alginolyticus is a Gram-negative bacterium commonly associated with mackerel poisoning. A bacteriophage that specifically targets and lyses this bacterium could be employed as a biocontrol agent for treating the bacterial infection or improving the shelf-life of mackerel products. However, only a few well-characterized V. alginolyticus phages have been reported in the literature. In this study, a novel lytic phage, named ΦImVa-1, specifically infecting V. alginolyticus strain ATCC 17749, was isolated from Indian mackerel. The phage has a short latent period of 15 min and a burst size of approximately 66 particles per infected bacterium. ΦImVa-1 remained stable for 2 h at a wide temperature (27–75 °C) and within a pH range of 5 to 10. Transmission electron microscopy revealed that ΦImVa-1 has an icosahedral head of approximately 60 nm in diameter with a short tail, resembling those in the Schitoviridae family. High throughput sequencing and bioinformatics analysis elucidated that ΦImVa-1 has a linear dsDNA genome of 77,479 base pairs (bp), with a G + C content of ~ 38.72% and 110 predicted gene coding regions (106 open reading frames and four tRNAs). The genome contains an extremely large virion-associated RNA polymerase gene and two smaller non-virion-associated RNA polymerase genes, which are hallmarks of schitoviruses. No antibiotic genes were found in the ΦImVa-1 genome. This is the first paper describing the biological properties, morphology, and the complete genome of a V. alginolyticus-infecting schitovirus. When raw mackerel fish flesh slices were treated with ΦImVa-1, the pathogen loads reduced significantly, demonstrating the potential of the phage as a biocontrol agent for V. alginolyticus strain ATCC 17749 in the food. Graphical Abstract: [Figure not available: see fulltext.] Key points: • A novel schitovirus infecting Vibrio alginolyticus ATCC 17749 was isolated from Indian mackerel. • The complete genome of the phage was determined, analyzed, and compared with other phages. • The phage is heat stable making it a potential biocontrol agent in extreme environments. |
| first_indexed | 2025-11-15T13:58:54Z |
| format | Article |
| id | upm-108099 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:58:54Z |
| publishDate | 2023 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1080992024-09-24T07:45:45Z http://psasir.upm.edu.my/id/eprint/108099/ Genomic analysis and biological characterization of a novel Schitoviridae phage infecting Vibrio alginolyticus Tajuddin, Sofiah Khan, Asif M. Li, Chuin Chong Wong, Chuan Loo Jia, Sen Tan Ina-Salwany, Md Yasin Lau, Han Yih Kok, Lian Ho Mariatulqabtiah, Abdul Razak Wen, Siang Tan Abstract: Vibrio alginolyticus is a Gram-negative bacterium commonly associated with mackerel poisoning. A bacteriophage that specifically targets and lyses this bacterium could be employed as a biocontrol agent for treating the bacterial infection or improving the shelf-life of mackerel products. However, only a few well-characterized V. alginolyticus phages have been reported in the literature. In this study, a novel lytic phage, named ΦImVa-1, specifically infecting V. alginolyticus strain ATCC 17749, was isolated from Indian mackerel. The phage has a short latent period of 15 min and a burst size of approximately 66 particles per infected bacterium. ΦImVa-1 remained stable for 2 h at a wide temperature (27–75 °C) and within a pH range of 5 to 10. Transmission electron microscopy revealed that ΦImVa-1 has an icosahedral head of approximately 60 nm in diameter with a short tail, resembling those in the Schitoviridae family. High throughput sequencing and bioinformatics analysis elucidated that ΦImVa-1 has a linear dsDNA genome of 77,479 base pairs (bp), with a G + C content of ~ 38.72% and 110 predicted gene coding regions (106 open reading frames and four tRNAs). The genome contains an extremely large virion-associated RNA polymerase gene and two smaller non-virion-associated RNA polymerase genes, which are hallmarks of schitoviruses. No antibiotic genes were found in the ΦImVa-1 genome. This is the first paper describing the biological properties, morphology, and the complete genome of a V. alginolyticus-infecting schitovirus. When raw mackerel fish flesh slices were treated with ΦImVa-1, the pathogen loads reduced significantly, demonstrating the potential of the phage as a biocontrol agent for V. alginolyticus strain ATCC 17749 in the food. Graphical Abstract: [Figure not available: see fulltext.] Key points: • A novel schitovirus infecting Vibrio alginolyticus ATCC 17749 was isolated from Indian mackerel. • The complete genome of the phage was determined, analyzed, and compared with other phages. • The phage is heat stable making it a potential biocontrol agent in extreme environments. Springer 2023 Article PeerReviewed Tajuddin, Sofiah and Khan, Asif M. and Li, Chuin Chong and Wong, Chuan Loo and Jia, Sen Tan and Ina-Salwany, Md Yasin and Lau, Han Yih and Kok, Lian Ho and Mariatulqabtiah, Abdul Razak and Wen, Siang Tan (2023) Genomic analysis and biological characterization of a novel Schitoviridae phage infecting Vibrio alginolyticus. Applied Microbiology and Biotechnology, 107. pp. 749-768. ISSN 0175-7598; ESSN: 1432-0614 https://link.springer.com/article/10.1007/s00253-022-12312-3?error=cookies_not_supported&code=556a936c-271c-40c5-9f4e-386001575098 10.1007/s00253-022-12312-3 |
| spellingShingle | Tajuddin, Sofiah Khan, Asif M. Li, Chuin Chong Wong, Chuan Loo Jia, Sen Tan Ina-Salwany, Md Yasin Lau, Han Yih Kok, Lian Ho Mariatulqabtiah, Abdul Razak Wen, Siang Tan Genomic analysis and biological characterization of a novel Schitoviridae phage infecting Vibrio alginolyticus |
| title | Genomic analysis and biological characterization of a novel Schitoviridae phage infecting Vibrio alginolyticus |
| title_full | Genomic analysis and biological characterization of a novel Schitoviridae phage infecting Vibrio alginolyticus |
| title_fullStr | Genomic analysis and biological characterization of a novel Schitoviridae phage infecting Vibrio alginolyticus |
| title_full_unstemmed | Genomic analysis and biological characterization of a novel Schitoviridae phage infecting Vibrio alginolyticus |
| title_short | Genomic analysis and biological characterization of a novel Schitoviridae phage infecting Vibrio alginolyticus |
| title_sort | genomic analysis and biological characterization of a novel schitoviridae phage infecting vibrio alginolyticus |
| url | http://psasir.upm.edu.my/id/eprint/108099/ http://psasir.upm.edu.my/id/eprint/108099/ http://psasir.upm.edu.my/id/eprint/108099/ |