Field efficacy of a feed-based inactivated vaccine against vibriosis in Cage-Cultured Asian seabass, Lates calcarifer, in Malaysia
Vibrio spp. are important aquaculture pathogens that cause vibriosis, affecting large numbers of marine fish species. This study determines the field efficacy of a feed-based inactivated vaccine against vibriosis in cage-cultured Asian seabass. A total of 4800 Asian seabass, kept in a field environm...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
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Multidisciplinary Digital Publishing Institute
2022
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| Online Access: | http://psasir.upm.edu.my/id/eprint/108002/ |
| _version_ | 1848865047151902720 |
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| author | Amir-Danial, Zahaludin Saad, Mohd Zamri Amal, Mohammad Noor Azmai Annas, Salleh Mohamad, Aslah Jumria, Sutra Manchanayake, Tilusha Arbania, Ali Ina-Salwany, Md Yasin |
| author_facet | Amir-Danial, Zahaludin Saad, Mohd Zamri Amal, Mohammad Noor Azmai Annas, Salleh Mohamad, Aslah Jumria, Sutra Manchanayake, Tilusha Arbania, Ali Ina-Salwany, Md Yasin |
| author_sort | Amir-Danial, Zahaludin |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Vibrio spp. are important aquaculture pathogens that cause vibriosis, affecting large numbers of marine fish species. This study determines the field efficacy of a feed-based inactivated vaccine against vibriosis in cage-cultured Asian seabass. A total of 4800 Asian seabass, kept in a field environment, were separated equally into two groups (vaccinated and non-vaccinated) in duplicate. Fish of Group 1 were orally administered the feed-based vaccine on weeks 0 (prime vaccination), 2 (booster), and 6 (second booster) at 4% body weight, while the non-vaccinated fish of Group 2 were fed with a commercial formulated pellet without the vaccine. Fish gut, mucus, and serum were collected, the length and weight of the fish were noted, while the mortality was recorded at 2-week intervals for a period of 16 weeks. The non-specific lysozyme activities were significantly (p < 0.05) higher in the fish of Group 1 than the non-vaccinated fish of Group 2. Similarly, the specific IgM antibody levels in serum and mucus were significantly (p < 0.05) higher in Group 1 than in Group 2, as seen in the second week, with the highest level 8 weeks after primary immunization. At week 16, the growth performance was significantly (p < 0.05) better in Group 1 and showed lower bacterial isolation in the gut than Group 2. Despite the statistical insignificance (p > 0.05), the survival rate was slightly higher in Group 1 (71.3%) than Group 2 (67.7%). This study revealed that feed-based vaccination improves growth performance, stimulates innate and adaptive immune responses, and increases protection of cultured Asian seabass, L. calcarifer, against vibriosis. |
| first_indexed | 2025-11-15T13:58:29Z |
| format | Article |
| id | upm-108002 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:58:29Z |
| publishDate | 2022 |
| publisher | Multidisciplinary Digital Publishing Institute |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1080022024-09-26T04:14:49Z http://psasir.upm.edu.my/id/eprint/108002/ Field efficacy of a feed-based inactivated vaccine against vibriosis in Cage-Cultured Asian seabass, Lates calcarifer, in Malaysia Amir-Danial, Zahaludin Saad, Mohd Zamri Amal, Mohammad Noor Azmai Annas, Salleh Mohamad, Aslah Jumria, Sutra Manchanayake, Tilusha Arbania, Ali Ina-Salwany, Md Yasin Vibrio spp. are important aquaculture pathogens that cause vibriosis, affecting large numbers of marine fish species. This study determines the field efficacy of a feed-based inactivated vaccine against vibriosis in cage-cultured Asian seabass. A total of 4800 Asian seabass, kept in a field environment, were separated equally into two groups (vaccinated and non-vaccinated) in duplicate. Fish of Group 1 were orally administered the feed-based vaccine on weeks 0 (prime vaccination), 2 (booster), and 6 (second booster) at 4% body weight, while the non-vaccinated fish of Group 2 were fed with a commercial formulated pellet without the vaccine. Fish gut, mucus, and serum were collected, the length and weight of the fish were noted, while the mortality was recorded at 2-week intervals for a period of 16 weeks. The non-specific lysozyme activities were significantly (p < 0.05) higher in the fish of Group 1 than the non-vaccinated fish of Group 2. Similarly, the specific IgM antibody levels in serum and mucus were significantly (p < 0.05) higher in Group 1 than in Group 2, as seen in the second week, with the highest level 8 weeks after primary immunization. At week 16, the growth performance was significantly (p < 0.05) better in Group 1 and showed lower bacterial isolation in the gut than Group 2. Despite the statistical insignificance (p > 0.05), the survival rate was slightly higher in Group 1 (71.3%) than Group 2 (67.7%). This study revealed that feed-based vaccination improves growth performance, stimulates innate and adaptive immune responses, and increases protection of cultured Asian seabass, L. calcarifer, against vibriosis. Multidisciplinary Digital Publishing Institute 2022 Article PeerReviewed Amir-Danial, Zahaludin and Saad, Mohd Zamri and Amal, Mohammad Noor Azmai and Annas, Salleh and Mohamad, Aslah and Jumria, Sutra and Manchanayake, Tilusha and Arbania, Ali and Ina-Salwany, Md Yasin (2022) Field efficacy of a feed-based inactivated vaccine against vibriosis in Cage-Cultured Asian seabass, Lates calcarifer, in Malaysia. Vaccines, 11 (1). art. no. 9. pp. 1-13. ISSN 2076-393X https://www.mdpi.com/2076-393X/11/1/9 10.3390/vaccines11010009 |
| spellingShingle | Amir-Danial, Zahaludin Saad, Mohd Zamri Amal, Mohammad Noor Azmai Annas, Salleh Mohamad, Aslah Jumria, Sutra Manchanayake, Tilusha Arbania, Ali Ina-Salwany, Md Yasin Field efficacy of a feed-based inactivated vaccine against vibriosis in Cage-Cultured Asian seabass, Lates calcarifer, in Malaysia |
| title | Field efficacy of a feed-based inactivated vaccine against vibriosis in Cage-Cultured Asian seabass, Lates calcarifer, in Malaysia |
| title_full | Field efficacy of a feed-based inactivated vaccine against vibriosis in Cage-Cultured Asian seabass, Lates calcarifer, in Malaysia |
| title_fullStr | Field efficacy of a feed-based inactivated vaccine against vibriosis in Cage-Cultured Asian seabass, Lates calcarifer, in Malaysia |
| title_full_unstemmed | Field efficacy of a feed-based inactivated vaccine against vibriosis in Cage-Cultured Asian seabass, Lates calcarifer, in Malaysia |
| title_short | Field efficacy of a feed-based inactivated vaccine against vibriosis in Cage-Cultured Asian seabass, Lates calcarifer, in Malaysia |
| title_sort | field efficacy of a feed-based inactivated vaccine against vibriosis in cage-cultured asian seabass, lates calcarifer, in malaysia |
| url | http://psasir.upm.edu.my/id/eprint/108002/ http://psasir.upm.edu.my/id/eprint/108002/ http://psasir.upm.edu.my/id/eprint/108002/ |