Evaluating the intestinal immunity of Asian Seabass (Lates calcarifer, bloch 1790) following field vaccination using a feed-based oral vaccine
This study describes the levels of gut lysozyme and IgM, the number, size and density of gut-associated lymphoid tissue (GALT) regions, and the lymphocyte population in Asian seabass following field oral administration of a feed-based vaccine. Fish in a grow-out farm were selected and divided into t...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Published: |
Multidisciplinary Digital Publishing Institute
2023
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/107761/ |
| _version_ | 1848864982927671296 |
|---|---|
| author | Raju, Thanusha Manchanayake, Tilusha Danial, Amir Zamri-Saad, Mohd Azmai, Mohammad Noor Amal Md Yasin, Ina Salwany Mohd Nor, Norhariani Salleh, Annas |
| author_facet | Raju, Thanusha Manchanayake, Tilusha Danial, Amir Zamri-Saad, Mohd Azmai, Mohammad Noor Amal Md Yasin, Ina Salwany Mohd Nor, Norhariani Salleh, Annas |
| author_sort | Raju, Thanusha |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | This study describes the levels of gut lysozyme and IgM, the number, size and density of gut-associated lymphoid tissue (GALT) regions, and the lymphocyte population in Asian seabass following field oral administration of a feed-based vaccine. Fish in a grow-out farm were selected and divided into two groups; Group 1 was vaccinated at week 0, 2, and 6, while Group 2 was not vaccinated. Samplings were done at 2-week intervals when the fish were observed for clinical signs, and gross lesions were recorded. The intestinal tissue and gut lavage fluid were collected. GALT regions (numbers, size, density and population of lymphocytes) were analyzed. Clinical signs such as abnormal swimming pattern and death, and gross lesions including scale loss, ocular opacity, and skin ulceration were observed in both groups. At the end of the study, the incidence rate between both groups were significantly different (p < 0.05). The gut IgM level and lysozyme activity, lymphocyte population, number, size and density of GALT regions of Group 1 were significantly (p < 0.05) higher than Group 2. Therefore, this study concludes that the feed-based vaccine reduces the incidence of vibriosis by stimulating the gut immunity of the vaccinated fish with an enhanced GALT region, specific IgM production against Vibrio harveyi, and lysozyme responses. |
| first_indexed | 2025-11-15T13:57:28Z |
| format | Article |
| id | upm-107761 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:57:28Z |
| publishDate | 2023 |
| publisher | Multidisciplinary Digital Publishing Institute |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1077612024-10-28T06:06:16Z http://psasir.upm.edu.my/id/eprint/107761/ Evaluating the intestinal immunity of Asian Seabass (Lates calcarifer, bloch 1790) following field vaccination using a feed-based oral vaccine Raju, Thanusha Manchanayake, Tilusha Danial, Amir Zamri-Saad, Mohd Azmai, Mohammad Noor Amal Md Yasin, Ina Salwany Mohd Nor, Norhariani Salleh, Annas This study describes the levels of gut lysozyme and IgM, the number, size and density of gut-associated lymphoid tissue (GALT) regions, and the lymphocyte population in Asian seabass following field oral administration of a feed-based vaccine. Fish in a grow-out farm were selected and divided into two groups; Group 1 was vaccinated at week 0, 2, and 6, while Group 2 was not vaccinated. Samplings were done at 2-week intervals when the fish were observed for clinical signs, and gross lesions were recorded. The intestinal tissue and gut lavage fluid were collected. GALT regions (numbers, size, density and population of lymphocytes) were analyzed. Clinical signs such as abnormal swimming pattern and death, and gross lesions including scale loss, ocular opacity, and skin ulceration were observed in both groups. At the end of the study, the incidence rate between both groups were significantly different (p < 0.05). The gut IgM level and lysozyme activity, lymphocyte population, number, size and density of GALT regions of Group 1 were significantly (p < 0.05) higher than Group 2. Therefore, this study concludes that the feed-based vaccine reduces the incidence of vibriosis by stimulating the gut immunity of the vaccinated fish with an enhanced GALT region, specific IgM production against Vibrio harveyi, and lysozyme responses. Multidisciplinary Digital Publishing Institute 2023-03-06 Article PeerReviewed Raju, Thanusha and Manchanayake, Tilusha and Danial, Amir and Zamri-Saad, Mohd and Azmai, Mohammad Noor Amal and Md Yasin, Ina Salwany and Mohd Nor, Norhariani and Salleh, Annas (2023) Evaluating the intestinal immunity of Asian Seabass (Lates calcarifer, bloch 1790) following field vaccination using a feed-based oral vaccine. Vaccines, 11 (3). pp. 602-616. ISSN 2076-393X https://www.mdpi.com/2076-393X/11/3/602 10.3390/vaccines11030602 |
| spellingShingle | Raju, Thanusha Manchanayake, Tilusha Danial, Amir Zamri-Saad, Mohd Azmai, Mohammad Noor Amal Md Yasin, Ina Salwany Mohd Nor, Norhariani Salleh, Annas Evaluating the intestinal immunity of Asian Seabass (Lates calcarifer, bloch 1790) following field vaccination using a feed-based oral vaccine |
| title | Evaluating the intestinal immunity of Asian Seabass (Lates calcarifer, bloch 1790) following field vaccination using a feed-based oral vaccine |
| title_full | Evaluating the intestinal immunity of Asian Seabass (Lates calcarifer, bloch 1790) following field vaccination using a feed-based oral vaccine |
| title_fullStr | Evaluating the intestinal immunity of Asian Seabass (Lates calcarifer, bloch 1790) following field vaccination using a feed-based oral vaccine |
| title_full_unstemmed | Evaluating the intestinal immunity of Asian Seabass (Lates calcarifer, bloch 1790) following field vaccination using a feed-based oral vaccine |
| title_short | Evaluating the intestinal immunity of Asian Seabass (Lates calcarifer, bloch 1790) following field vaccination using a feed-based oral vaccine |
| title_sort | evaluating the intestinal immunity of asian seabass (lates calcarifer, bloch 1790) following field vaccination using a feed-based oral vaccine |
| url | http://psasir.upm.edu.my/id/eprint/107761/ http://psasir.upm.edu.my/id/eprint/107761/ http://psasir.upm.edu.my/id/eprint/107761/ |