Enhanced apoptotic activity of Pluronic F127 polymer-encapsulated chlorogenic acid nanoparticles through the PI3K/Akt/mTOR signaling pathway in liver cancer cells and in vivo toxicity studies in zebrafish
In this study, chlorogenic acid nanoparticles encapsulated in Pluronic F127 polymer were synthesized and characterized to determine if they could treat human liver cancer. The nanoparticles were synthesized using standard procedures and characterized using physical and biological techniques such as...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
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De Gruyter
2023
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| Online Access: | http://psasir.upm.edu.my/id/eprint/107684/ |
| _version_ | 1848864962772992000 |
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| author | Alanazi, Fehaid Elderdery, Abozer Y. Alzahrani, Badr Alzerwi, Nasser A. N. Althobiti, Maryam Musleh Rayzah, Musaed Alanazi, Abdulaziz Suailem Kuriri, Fahd A. Idrees, Bandar Alenazy, Fawaz O. Alsultan, Afnan Rayzah, Fares Baksh, Yaser Subbiah, Suresh Kumar Mok, Pooi Ling |
| author_facet | Alanazi, Fehaid Elderdery, Abozer Y. Alzahrani, Badr Alzerwi, Nasser A. N. Althobiti, Maryam Musleh Rayzah, Musaed Alanazi, Abdulaziz Suailem Kuriri, Fahd A. Idrees, Bandar Alenazy, Fawaz O. Alsultan, Afnan Rayzah, Fares Baksh, Yaser Subbiah, Suresh Kumar Mok, Pooi Ling |
| author_sort | Alanazi, Fehaid |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | In this study, chlorogenic acid nanoparticles encapsulated in Pluronic F127 polymer were synthesized and characterized to determine if they could treat human liver cancer. The nanoparticles were synthesized using standard procedures and characterized using physical and biological techniques such as X-ray diffraction, Fourier transform infrared spectroscopy, UV-Vis, dynamic light scattering, Photoluminescence, scanning electron microscopy, and transmission electron microscopy. The anticancer effects were assessed using MTT analysis, acridine orange/ethidium bromide, reactive oxygen species (ROS), COMET assay, annexinV/FITC, cell cycle analysis, and expression of marker genes against HepG2 cell lines. The results showed significant cytotoxicity, apoptosis induction, and increased ROS production in treated cells compared to control cells. The nanoparticles also activated the apoptotic cascade and regulated the PI3K/AKT/mTOR pathways. The nanocomposites exhibited unique characteristics such as anticancer efficacy in vitro. Further research was conducted using zebrafish to model hematological parameters, liver enzymes, and histopathology to study effectiveness. Green-synthesized Pluronic F127“chlorogenic acid nanoparticles can be considered potential cancer therapy agents. |
| first_indexed | 2025-11-15T13:57:09Z |
| format | Article |
| id | upm-107684 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:57:09Z |
| publishDate | 2023 |
| publisher | De Gruyter |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1076842024-11-05T01:21:38Z http://psasir.upm.edu.my/id/eprint/107684/ Enhanced apoptotic activity of Pluronic F127 polymer-encapsulated chlorogenic acid nanoparticles through the PI3K/Akt/mTOR signaling pathway in liver cancer cells and in vivo toxicity studies in zebrafish Alanazi, Fehaid Elderdery, Abozer Y. Alzahrani, Badr Alzerwi, Nasser A. N. Althobiti, Maryam Musleh Rayzah, Musaed Alanazi, Abdulaziz Suailem Kuriri, Fahd A. Idrees, Bandar Alenazy, Fawaz O. Alsultan, Afnan Rayzah, Fares Baksh, Yaser Subbiah, Suresh Kumar Mok, Pooi Ling In this study, chlorogenic acid nanoparticles encapsulated in Pluronic F127 polymer were synthesized and characterized to determine if they could treat human liver cancer. The nanoparticles were synthesized using standard procedures and characterized using physical and biological techniques such as X-ray diffraction, Fourier transform infrared spectroscopy, UV-Vis, dynamic light scattering, Photoluminescence, scanning electron microscopy, and transmission electron microscopy. The anticancer effects were assessed using MTT analysis, acridine orange/ethidium bromide, reactive oxygen species (ROS), COMET assay, annexinV/FITC, cell cycle analysis, and expression of marker genes against HepG2 cell lines. The results showed significant cytotoxicity, apoptosis induction, and increased ROS production in treated cells compared to control cells. The nanoparticles also activated the apoptotic cascade and regulated the PI3K/AKT/mTOR pathways. The nanocomposites exhibited unique characteristics such as anticancer efficacy in vitro. Further research was conducted using zebrafish to model hematological parameters, liver enzymes, and histopathology to study effectiveness. Green-synthesized Pluronic F127“chlorogenic acid nanoparticles can be considered potential cancer therapy agents. De Gruyter 2023-11-03 Article PeerReviewed Alanazi, Fehaid and Elderdery, Abozer Y. and Alzahrani, Badr and Alzerwi, Nasser A. N. and Althobiti, Maryam Musleh and Rayzah, Musaed and Alanazi, Abdulaziz Suailem and Kuriri, Fahd A. and Idrees, Bandar and Alenazy, Fawaz O. and Alsultan, Afnan and Rayzah, Fares and Baksh, Yaser and Subbiah, Suresh Kumar and Mok, Pooi Ling (2023) Enhanced apoptotic activity of Pluronic F127 polymer-encapsulated chlorogenic acid nanoparticles through the PI3K/Akt/mTOR signaling pathway in liver cancer cells and in vivo toxicity studies in zebrafish. E-polymers, 23 (1). pp. 1-15. ISSN 1618-7229 https://www.degruyter.com/document/doi/10.1515/epoly-2023-0053/html 10.1515/epoly-2023-0053 |
| spellingShingle | Alanazi, Fehaid Elderdery, Abozer Y. Alzahrani, Badr Alzerwi, Nasser A. N. Althobiti, Maryam Musleh Rayzah, Musaed Alanazi, Abdulaziz Suailem Kuriri, Fahd A. Idrees, Bandar Alenazy, Fawaz O. Alsultan, Afnan Rayzah, Fares Baksh, Yaser Subbiah, Suresh Kumar Mok, Pooi Ling Enhanced apoptotic activity of Pluronic F127 polymer-encapsulated chlorogenic acid nanoparticles through the PI3K/Akt/mTOR signaling pathway in liver cancer cells and in vivo toxicity studies in zebrafish |
| title | Enhanced apoptotic activity of Pluronic F127 polymer-encapsulated chlorogenic acid nanoparticles through the PI3K/Akt/mTOR signaling pathway in liver cancer cells and in vivo toxicity studies in zebrafish |
| title_full | Enhanced apoptotic activity of Pluronic F127 polymer-encapsulated chlorogenic acid nanoparticles through the PI3K/Akt/mTOR signaling pathway in liver cancer cells and in vivo toxicity studies in zebrafish |
| title_fullStr | Enhanced apoptotic activity of Pluronic F127 polymer-encapsulated chlorogenic acid nanoparticles through the PI3K/Akt/mTOR signaling pathway in liver cancer cells and in vivo toxicity studies in zebrafish |
| title_full_unstemmed | Enhanced apoptotic activity of Pluronic F127 polymer-encapsulated chlorogenic acid nanoparticles through the PI3K/Akt/mTOR signaling pathway in liver cancer cells and in vivo toxicity studies in zebrafish |
| title_short | Enhanced apoptotic activity of Pluronic F127 polymer-encapsulated chlorogenic acid nanoparticles through the PI3K/Akt/mTOR signaling pathway in liver cancer cells and in vivo toxicity studies in zebrafish |
| title_sort | enhanced apoptotic activity of pluronic f127 polymer-encapsulated chlorogenic acid nanoparticles through the pi3k/akt/mtor signaling pathway in liver cancer cells and in vivo toxicity studies in zebrafish |
| url | http://psasir.upm.edu.my/id/eprint/107684/ http://psasir.upm.edu.my/id/eprint/107684/ http://psasir.upm.edu.my/id/eprint/107684/ |