Effect of indomethacin on embryo implantation and histomorphology of uterus, ovary, kidney, and liver of rats
Background This study aimed to determine the effects of Indomethacin (IMC) treatment on embryo implantation and histomorphology of uterus, ovary, and other vital organs and its effective dosage in establishing embryo implantation dysfunction model in Sprague-Dawley (SD) rats. Materials and Metho...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023
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| Online Access: | http://psasir.upm.edu.my/id/eprint/107510/ http://psasir.upm.edu.my/id/eprint/107510/1/Effect%20of%20indomethacin%20on%20embryo%20implantation%20and%20histomorphology%20of%20uterus%2C%20ovary%2C%20kidney%2C%20and%20liver%20of%20rats.pdf |
| _version_ | 1848864914943246336 |
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| author | Amir, Maria Yimer, Nurhusien Hiew, Mark Mohd Yusoff, Sabri Hussen, Bedru Quddus, Abdul |
| author_facet | Amir, Maria Yimer, Nurhusien Hiew, Mark Mohd Yusoff, Sabri Hussen, Bedru Quddus, Abdul |
| author_sort | Amir, Maria |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Background
This study aimed to determine the effects of Indomethacin (IMC) treatment on embryo implantation and histomorphology of uterus, ovary, and other vital organs and its effective dosage in establishing embryo implantation dysfunction model in Sprague-Dawley (SD) rats.
Materials and Methods
The experiments were performed on 24 (6 × 4 groups) adult female SD rats aged 12 weeks old. G1 was the control group and received a normal diet with normal saline. However, on pregnancy days 3 (Pd3) and 4 (Pd4), G2, G3, and G4 were given normal saline and subcutaneously administered IMC twice daily at different doses of 4.33, 4.66 and 5.00 mg/kg body weight, respectively. The rats were euthanized on day 8 of pregnancy (Pd8). The uterus was excised and examined for signs of pregnancy, followed by tissue samples from liver, kidney, and ovary (for histomorphological examination using haematoxylin and eosin stain).
Results
All IMC treatment doses disrupted the implantation process and caused a significant reduction in embryo development. Analysis for histopathological changes revealed that IMC doses above 4.33 mg/kg body weight caused more adverse reproductive health effects in rats. Vasoconstriction and micro vascularization were detected in the liver, while degenerative Bowman's capsules and inflammatory cells were observed in kidney sections from IMC-treated rats.
Conclusion
IMC therapy interfered with implantation and embryo development in rats, resulting in significant uterine vasoconstriction and atrophy, 4.33 mg/kg bwt dose appeared to be optimum to establish embryo implantation dysfunction in SD rats. |
| first_indexed | 2025-11-15T13:56:23Z |
| format | Article |
| id | upm-107510 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T13:56:23Z |
| publishDate | 2023 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1075102024-10-17T07:35:43Z http://psasir.upm.edu.my/id/eprint/107510/ Effect of indomethacin on embryo implantation and histomorphology of uterus, ovary, kidney, and liver of rats Amir, Maria Yimer, Nurhusien Hiew, Mark Mohd Yusoff, Sabri Hussen, Bedru Quddus, Abdul Background This study aimed to determine the effects of Indomethacin (IMC) treatment on embryo implantation and histomorphology of uterus, ovary, and other vital organs and its effective dosage in establishing embryo implantation dysfunction model in Sprague-Dawley (SD) rats. Materials and Methods The experiments were performed on 24 (6 × 4 groups) adult female SD rats aged 12 weeks old. G1 was the control group and received a normal diet with normal saline. However, on pregnancy days 3 (Pd3) and 4 (Pd4), G2, G3, and G4 were given normal saline and subcutaneously administered IMC twice daily at different doses of 4.33, 4.66 and 5.00 mg/kg body weight, respectively. The rats were euthanized on day 8 of pregnancy (Pd8). The uterus was excised and examined for signs of pregnancy, followed by tissue samples from liver, kidney, and ovary (for histomorphological examination using haematoxylin and eosin stain). Results All IMC treatment doses disrupted the implantation process and caused a significant reduction in embryo development. Analysis for histopathological changes revealed that IMC doses above 4.33 mg/kg body weight caused more adverse reproductive health effects in rats. Vasoconstriction and micro vascularization were detected in the liver, while degenerative Bowman's capsules and inflammatory cells were observed in kidney sections from IMC-treated rats. Conclusion IMC therapy interfered with implantation and embryo development in rats, resulting in significant uterine vasoconstriction and atrophy, 4.33 mg/kg bwt dose appeared to be optimum to establish embryo implantation dysfunction in SD rats. Elsevier 2023-12 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/107510/1/Effect%20of%20indomethacin%20on%20embryo%20implantation%20and%20histomorphology%20of%20uterus%2C%20ovary%2C%20kidney%2C%20and%20liver%20of%20rats.pdf Amir, Maria and Yimer, Nurhusien and Hiew, Mark and Mohd Yusoff, Sabri and Hussen, Bedru and Quddus, Abdul (2023) Effect of indomethacin on embryo implantation and histomorphology of uterus, ovary, kidney, and liver of rats. Saudi Journal of Biological Sciences, 30 (12). art. no. 103837. pp. 1-10. ISSN 1319-562X https://www.sciencedirect.com/science/article/pii/S1319562X23002826?via%3Dihub 10.1016/j.sjbs.2023.103837 |
| spellingShingle | Amir, Maria Yimer, Nurhusien Hiew, Mark Mohd Yusoff, Sabri Hussen, Bedru Quddus, Abdul Effect of indomethacin on embryo implantation and histomorphology of uterus, ovary, kidney, and liver of rats |
| title | Effect of indomethacin on embryo implantation and histomorphology of uterus, ovary, kidney, and liver of rats |
| title_full | Effect of indomethacin on embryo implantation and histomorphology of uterus, ovary, kidney, and liver of rats |
| title_fullStr | Effect of indomethacin on embryo implantation and histomorphology of uterus, ovary, kidney, and liver of rats |
| title_full_unstemmed | Effect of indomethacin on embryo implantation and histomorphology of uterus, ovary, kidney, and liver of rats |
| title_short | Effect of indomethacin on embryo implantation and histomorphology of uterus, ovary, kidney, and liver of rats |
| title_sort | effect of indomethacin on embryo implantation and histomorphology of uterus, ovary, kidney, and liver of rats |
| url | http://psasir.upm.edu.my/id/eprint/107510/ http://psasir.upm.edu.my/id/eprint/107510/ http://psasir.upm.edu.my/id/eprint/107510/ http://psasir.upm.edu.my/id/eprint/107510/1/Effect%20of%20indomethacin%20on%20embryo%20implantation%20and%20histomorphology%20of%20uterus%2C%20ovary%2C%20kidney%2C%20and%20liver%20of%20rats.pdf |