Effect of acrylamide on the degradation of palm-based used cooking oil by single and co-culture isolates of Alcaligenes sp. AQ5-02 and Serratia sp. AQ5-03
Acrylamide is found in the environment, food, and waste products such as palm-based used cooking oil (UCO). The presence of acrylamide is a threat due to its neurotoxic, genotoxic, teratogenic, and carcinogenic characteristics. These negative impacts have sparked an interest in microbe-mediated bior...
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| Format: | Article |
| Language: | English |
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Malaysian Society for Biochemistry and Molecular Biology
2023
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| Online Access: | http://psasir.upm.edu.my/id/eprint/107479/ http://psasir.upm.edu.my/id/eprint/107479/1/Effect%20of%20acrylamide%20on%20the%20degradation%20of%20palm-based%20used%20cooking%20oil%20by%20single%20and%20co-culture%20isolates%20of%20Alcaligenes%20sp.%20AQ5-02%20and%20Serratia%20sp.%20AQ5-03.pdf |
| _version_ | 1848864907207901184 |
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| author | Ahmed, Mariyam Shabeea Ahmad, Siti Aqlima Shukor, Mohd Yunus Yusof, Mohd Termizi |
| author_facet | Ahmed, Mariyam Shabeea Ahmad, Siti Aqlima Shukor, Mohd Yunus Yusof, Mohd Termizi |
| author_sort | Ahmed, Mariyam Shabeea |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Acrylamide is found in the environment, food, and waste products such as palm-based used cooking oil (UCO). The presence of acrylamide is a threat due to its neurotoxic, genotoxic, teratogenic, and carcinogenic characteristics. These negative impacts have sparked an interest in microbe-mediated bioremediation of acrylamide. The aim of this study is to investigate the effect of various concentrations of acrylamide on the degradation of UCO as a sole carbon source by single and co-culture isolates of Alcaligenes sp. AQ5-02 and Serratia sp. AQ5-03. Using gravimetric analysis, different acrylamide concentrations ranging from 10-200 mg/L were shown to significantly reduce palm oil UCO degradation and bacterial growth in single and co-culture formulations. In the co-culture, there were significant differences between all acrylamide concentrations in terms of UCO degradation. The single and co-culture isolates were able to withstand 10 mg/L of acrylamide while degrading more than 50 of UCO. However, beyond 10 mg/L, there was a significant reduction in the degradation of UCO in both single and co-culture methods. Two-way ANOVA reveals a significant difference in the degradation and bacterial growth between the single and co-culture isolates of Alcaligenes sp. AQ5-02 and Serratia sp. AQ5-03 with varying acrylamide concentrations (p < 0.0001). In conclusion, the co-culture has greater degradation and better tolerance at all concentrations of acrylamide compared to single isolate cultures of Alcaligenes sp. AQ5-02 and Serratia sp. AQ5-03. The study provides insightful knowledge on the presence of xenobiotics in the bioremediation of hydrocarbons by single and co-culture. |
| first_indexed | 2025-11-15T13:56:16Z |
| format | Article |
| id | upm-107479 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T13:56:16Z |
| publishDate | 2023 |
| publisher | Malaysian Society for Biochemistry and Molecular Biology |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1074792024-10-17T03:39:44Z http://psasir.upm.edu.my/id/eprint/107479/ Effect of acrylamide on the degradation of palm-based used cooking oil by single and co-culture isolates of Alcaligenes sp. AQ5-02 and Serratia sp. AQ5-03 Ahmed, Mariyam Shabeea Ahmad, Siti Aqlima Shukor, Mohd Yunus Yusof, Mohd Termizi Acrylamide is found in the environment, food, and waste products such as palm-based used cooking oil (UCO). The presence of acrylamide is a threat due to its neurotoxic, genotoxic, teratogenic, and carcinogenic characteristics. These negative impacts have sparked an interest in microbe-mediated bioremediation of acrylamide. The aim of this study is to investigate the effect of various concentrations of acrylamide on the degradation of UCO as a sole carbon source by single and co-culture isolates of Alcaligenes sp. AQ5-02 and Serratia sp. AQ5-03. Using gravimetric analysis, different acrylamide concentrations ranging from 10-200 mg/L were shown to significantly reduce palm oil UCO degradation and bacterial growth in single and co-culture formulations. In the co-culture, there were significant differences between all acrylamide concentrations in terms of UCO degradation. The single and co-culture isolates were able to withstand 10 mg/L of acrylamide while degrading more than 50 of UCO. However, beyond 10 mg/L, there was a significant reduction in the degradation of UCO in both single and co-culture methods. Two-way ANOVA reveals a significant difference in the degradation and bacterial growth between the single and co-culture isolates of Alcaligenes sp. AQ5-02 and Serratia sp. AQ5-03 with varying acrylamide concentrations (p < 0.0001). In conclusion, the co-culture has greater degradation and better tolerance at all concentrations of acrylamide compared to single isolate cultures of Alcaligenes sp. AQ5-02 and Serratia sp. AQ5-03. The study provides insightful knowledge on the presence of xenobiotics in the bioremediation of hydrocarbons by single and co-culture. Malaysian Society for Biochemistry and Molecular Biology 2023-04 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/107479/1/Effect%20of%20acrylamide%20on%20the%20degradation%20of%20palm-based%20used%20cooking%20oil%20by%20single%20and%20co-culture%20isolates%20of%20Alcaligenes%20sp.%20AQ5-02%20and%20Serratia%20sp.%20AQ5-03.pdf Ahmed, Mariyam Shabeea and Ahmad, Siti Aqlima and Shukor, Mohd Yunus and Yusof, Mohd Termizi (2023) Effect of acrylamide on the degradation of palm-based used cooking oil by single and co-culture isolates of Alcaligenes sp. AQ5-02 and Serratia sp. AQ5-03. Malaysian Journal of Biochemistry and Molecular Biology, 26 (1). 42 - 50. ISSN 2600-9005 https://1f169227-0328-4af2-99bc-24934f131be8.filesusr.com/ugd/66925b_61bb3c6c5bd64a4d92b1ad1f040b3b79.pdf |
| spellingShingle | Ahmed, Mariyam Shabeea Ahmad, Siti Aqlima Shukor, Mohd Yunus Yusof, Mohd Termizi Effect of acrylamide on the degradation of palm-based used cooking oil by single and co-culture isolates of Alcaligenes sp. AQ5-02 and Serratia sp. AQ5-03 |
| title | Effect of acrylamide on the degradation of palm-based used cooking oil by single and co-culture isolates of Alcaligenes sp. AQ5-02 and Serratia sp. AQ5-03 |
| title_full | Effect of acrylamide on the degradation of palm-based used cooking oil by single and co-culture isolates of Alcaligenes sp. AQ5-02 and Serratia sp. AQ5-03 |
| title_fullStr | Effect of acrylamide on the degradation of palm-based used cooking oil by single and co-culture isolates of Alcaligenes sp. AQ5-02 and Serratia sp. AQ5-03 |
| title_full_unstemmed | Effect of acrylamide on the degradation of palm-based used cooking oil by single and co-culture isolates of Alcaligenes sp. AQ5-02 and Serratia sp. AQ5-03 |
| title_short | Effect of acrylamide on the degradation of palm-based used cooking oil by single and co-culture isolates of Alcaligenes sp. AQ5-02 and Serratia sp. AQ5-03 |
| title_sort | effect of acrylamide on the degradation of palm-based used cooking oil by single and co-culture isolates of alcaligenes sp. aq5-02 and serratia sp. aq5-03 |
| url | http://psasir.upm.edu.my/id/eprint/107479/ http://psasir.upm.edu.my/id/eprint/107479/ http://psasir.upm.edu.my/id/eprint/107479/1/Effect%20of%20acrylamide%20on%20the%20degradation%20of%20palm-based%20used%20cooking%20oil%20by%20single%20and%20co-culture%20isolates%20of%20Alcaligenes%20sp.%20AQ5-02%20and%20Serratia%20sp.%20AQ5-03.pdf |