Detection and antibiotic resistance profile of extended-spectrum betalactamase—producing Escherichia coli in raw vegetables
The increase of antibiotic-resistance bacteria in food and vegetables has heightened the concern related to food safety globally. The current study aimed to determine the prevalence and antibiotic resistance profile of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli in raw vegetab...
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| Format: | Article |
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Rynnye Lyan Resources
2023
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| Online Access: | http://psasir.upm.edu.my/id/eprint/107248/ |
| _version_ | 1848864868726210560 |
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| author | Lee, E. New, C. Y. Thung, T. Y. Tan, C. W. Wendy R. D. J., Nuzul N. Son, R. Abdul-Mutalib, N. A. |
| author_facet | Lee, E. New, C. Y. Thung, T. Y. Tan, C. W. Wendy R. D. J., Nuzul N. Son, R. Abdul-Mutalib, N. A. |
| author_sort | Lee, E. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The increase of antibiotic-resistance bacteria in food and vegetables has heightened the concern related to food safety globally. The current study aimed to determine the prevalence and antibiotic resistance profile of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli in raw vegetables. This study coupled the most probable number (MPN) method with multiplex polymerase chain reaction (mPCR) to determine the presence and enumerate the ESBL-producing E. coli in raw vegetables. The result showed that the prevalence of the isolates in raw vegetables was 62.78 (113/180), consisting of 62.11 (59/95) in green carol lettuce and 63.53 (54/85) in mung bean sprouts, whereas the microbial load ranged from 1100 MPN/g with median 9.2 MPN/g. Overall, the study showed that there was no significant difference (P>0.05) in the microbial density of the isolates in vegetable samples purchased from wet markets and hypermarkets. Furthermore, the antibiotic susceptibility test revealed that all related strains were susceptible to cefepime, piperacillin/tazobactam and meropenem. However, the resistance to ampicillin was shown by 80 of the isolates. The multiple antibiotic resistance (MAR) indices of ESBL-producing E. coli ranged from 0.1 to 0.6. The majority of the isolates (60) showed multidrug resistance. Hence, the current study suggested that raw vegetables could be a vehicle for the transmission of ESBL-producing E. coli to humans. |
| first_indexed | 2025-11-15T13:55:39Z |
| format | Article |
| id | upm-107248 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:55:39Z |
| publishDate | 2023 |
| publisher | Rynnye Lyan Resources |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1072482024-10-17T01:56:56Z http://psasir.upm.edu.my/id/eprint/107248/ Detection and antibiotic resistance profile of extended-spectrum betalactamase—producing Escherichia coli in raw vegetables Lee, E. New, C. Y. Thung, T. Y. Tan, C. W. Wendy R. D. J., Nuzul N. Son, R. Abdul-Mutalib, N. A. The increase of antibiotic-resistance bacteria in food and vegetables has heightened the concern related to food safety globally. The current study aimed to determine the prevalence and antibiotic resistance profile of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli in raw vegetables. This study coupled the most probable number (MPN) method with multiplex polymerase chain reaction (mPCR) to determine the presence and enumerate the ESBL-producing E. coli in raw vegetables. The result showed that the prevalence of the isolates in raw vegetables was 62.78 (113/180), consisting of 62.11 (59/95) in green carol lettuce and 63.53 (54/85) in mung bean sprouts, whereas the microbial load ranged from 1100 MPN/g with median 9.2 MPN/g. Overall, the study showed that there was no significant difference (P>0.05) in the microbial density of the isolates in vegetable samples purchased from wet markets and hypermarkets. Furthermore, the antibiotic susceptibility test revealed that all related strains were susceptible to cefepime, piperacillin/tazobactam and meropenem. However, the resistance to ampicillin was shown by 80 of the isolates. The multiple antibiotic resistance (MAR) indices of ESBL-producing E. coli ranged from 0.1 to 0.6. The majority of the isolates (60) showed multidrug resistance. Hence, the current study suggested that raw vegetables could be a vehicle for the transmission of ESBL-producing E. coli to humans. Rynnye Lyan Resources 2023 Article PeerReviewed Lee, E. and New, C. Y. and Thung, T. Y. and Tan, C. W. and Wendy R. D. and J., Nuzul N. and Son, R. and Abdul-Mutalib, N. A. (2023) Detection and antibiotic resistance profile of extended-spectrum betalactamase—producing Escherichia coli in raw vegetables. Food Research, 7 (4). pp. 45-54. ISSN 2550-2166 https://www.myfoodresearch.com/vol-79474issue-4.html 10.26656/fr.2017.7(4).E1 |
| spellingShingle | Lee, E. New, C. Y. Thung, T. Y. Tan, C. W. Wendy R. D. J., Nuzul N. Son, R. Abdul-Mutalib, N. A. Detection and antibiotic resistance profile of extended-spectrum betalactamase—producing Escherichia coli in raw vegetables |
| title | Detection and antibiotic resistance profile of extended-spectrum betalactamase—producing Escherichia coli in raw vegetables |
| title_full | Detection and antibiotic resistance profile of extended-spectrum betalactamase—producing Escherichia coli in raw vegetables |
| title_fullStr | Detection and antibiotic resistance profile of extended-spectrum betalactamase—producing Escherichia coli in raw vegetables |
| title_full_unstemmed | Detection and antibiotic resistance profile of extended-spectrum betalactamase—producing Escherichia coli in raw vegetables |
| title_short | Detection and antibiotic resistance profile of extended-spectrum betalactamase—producing Escherichia coli in raw vegetables |
| title_sort | detection and antibiotic resistance profile of extended-spectrum betalactamase—producing escherichia coli in raw vegetables |
| url | http://psasir.upm.edu.my/id/eprint/107248/ http://psasir.upm.edu.my/id/eprint/107248/ http://psasir.upm.edu.my/id/eprint/107248/ |