Comparative genomics analysis of Ganoderma orthologs involved in plant-pathogenesis

Ganoderma species are producers of bioactive secondary metabolites and lignin degraders. A few Ganoderma species are known to be plant pathogens that attack economically important trees. In this study, comparative genomics analysis was conducted on the proteome of ten Ganoderma species/strains, focu...

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Main Author: Ho, Chai-Ling
Format: Article
Language:English
Published: Multidisciplinary Digital Publishing Institute 2023
Online Access:http://psasir.upm.edu.my/id/eprint/107078/
http://psasir.upm.edu.my/id/eprint/107078/1/Comparative%20genomics%20analysis%20of%20Ganoderma%20orthologs%20involved%20in%20plant-pathogenesis.pdf
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author Ho, Chai-Ling
author_facet Ho, Chai-Ling
author_sort Ho, Chai-Ling
building UPM Institutional Repository
collection Online Access
description Ganoderma species are producers of bioactive secondary metabolites and lignin degraders. A few Ganoderma species are known to be plant pathogens that attack economically important trees. In this study, comparative genomics analysis was conducted on the proteome of ten Ganoderma species/strains, focusing on the proteins that have been reported to be involved in plant-pathogenesis in other fungi. Fungal trophic lifestyle prediction of these Ganoderma species/strains supported that G. boninense (a potent pathogen to oil palm) is a hemibiotrophic fungus while the other Ganoderma species/strains analyzed were predicted to be saprophytes or a symbiont based on their Carbohydrate-Active Enzyme (CAZyme) contents. Although these Ganoderma species/strains were demonstrated to share many CAZymes and secondary metabolite core gene clusters, individual species may produce unique CAZymes and secondary metabolite core genes that determine their lifestyles, host-specificity, and potential as a producer of bioactive secondary metabolites. Ortholog groups that are related to fungal virulence from seven Ganoderma species/strains including those involved in lignin degradation, mycotoxin, siderophore and ergosterol biosynthesis, and virulence were summarized. Potential effectors were predicted from the proteome of these Ganoderma species/strains, and putative effectors that were being expressed in G. boninense in oil palm roots but not found in other species were identified. The findings provide a useful resource to further analyze plant-pathogenesis and wood degradation activities of these Ganoderma species.
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spelling upm-1070782024-10-17T07:06:11Z http://psasir.upm.edu.my/id/eprint/107078/ Comparative genomics analysis of Ganoderma orthologs involved in plant-pathogenesis Ho, Chai-Ling Ganoderma species are producers of bioactive secondary metabolites and lignin degraders. A few Ganoderma species are known to be plant pathogens that attack economically important trees. In this study, comparative genomics analysis was conducted on the proteome of ten Ganoderma species/strains, focusing on the proteins that have been reported to be involved in plant-pathogenesis in other fungi. Fungal trophic lifestyle prediction of these Ganoderma species/strains supported that G. boninense (a potent pathogen to oil palm) is a hemibiotrophic fungus while the other Ganoderma species/strains analyzed were predicted to be saprophytes or a symbiont based on their Carbohydrate-Active Enzyme (CAZyme) contents. Although these Ganoderma species/strains were demonstrated to share many CAZymes and secondary metabolite core gene clusters, individual species may produce unique CAZymes and secondary metabolite core genes that determine their lifestyles, host-specificity, and potential as a producer of bioactive secondary metabolites. Ortholog groups that are related to fungal virulence from seven Ganoderma species/strains including those involved in lignin degradation, mycotoxin, siderophore and ergosterol biosynthesis, and virulence were summarized. Potential effectors were predicted from the proteome of these Ganoderma species/strains, and putative effectors that were being expressed in G. boninense in oil palm roots but not found in other species were identified. The findings provide a useful resource to further analyze plant-pathogenesis and wood degradation activities of these Ganoderma species. Multidisciplinary Digital Publishing Institute 2023-03-22 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/107078/1/Comparative%20genomics%20analysis%20of%20Ganoderma%20orthologs%20involved%20in%20plant-pathogenesis.pdf Ho, Chai-Ling (2023) Comparative genomics analysis of Ganoderma orthologs involved in plant-pathogenesis. Forests, 14 (3). art. no. 653. pp. 1-20. ISSN 1999-4907 https://www.mdpi.com/1999-4907/14/3/653 10.3390/f14030653
spellingShingle Ho, Chai-Ling
Comparative genomics analysis of Ganoderma orthologs involved in plant-pathogenesis
title Comparative genomics analysis of Ganoderma orthologs involved in plant-pathogenesis
title_full Comparative genomics analysis of Ganoderma orthologs involved in plant-pathogenesis
title_fullStr Comparative genomics analysis of Ganoderma orthologs involved in plant-pathogenesis
title_full_unstemmed Comparative genomics analysis of Ganoderma orthologs involved in plant-pathogenesis
title_short Comparative genomics analysis of Ganoderma orthologs involved in plant-pathogenesis
title_sort comparative genomics analysis of ganoderma orthologs involved in plant-pathogenesis
url http://psasir.upm.edu.my/id/eprint/107078/
http://psasir.upm.edu.my/id/eprint/107078/
http://psasir.upm.edu.my/id/eprint/107078/
http://psasir.upm.edu.my/id/eprint/107078/1/Comparative%20genomics%20analysis%20of%20Ganoderma%20orthologs%20involved%20in%20plant-pathogenesis.pdf